Synthesis, delineation and technological advancements of algae biochar for sustainable remediation of the emerging pollutants from wastewater-a review.

The use of algae for value-added product and biorefining applications is enchanting attention among researchers in recent years due to its remarkable photosynthetic ability, adaptability, and capacity to accumulate lipids and carbohydrates. Algae biomass, based on its low manufacturing costs, is relatively renewable, sustainable, environmentally friendly and economical in comparison with other species. High production rate of algae provides a unique opportunity for its conversion to biochar with excellent physicochemical properties, viz. high surface area and pore volume, high adsorption capacity, abundant functional groups over surface, etc. Despite several potential algal-biochar, a detailed study on its application for removal of emerging contaminants from wastewater is limited. Therefore, this technical review is being carried out to evaluate the specific elimination of inorganic and organic pollutants from wastewater, with a view to assessing adsorption performances of biochar obtained from various algae species. Species-specific adsorption of emerging pollutants from wastewater have been discussed in the present review. The promising methods like pyrolysis, gasification, dry and wet torrefaction for the production of algae biochar are highlighted. The strategies include chemical and structural modifications of algae biochar for the removal of toxic contaminants have also been considered in the current work. The overall aim of this review is to confer about the synthesis, technological advancements, delineation and application of algae biochar for the treatment of wastewater.

An Evidence of Carbonic Anhydrase Activity in Native Microalgae for CO2 Capture Application.

A promising alternative for effective carbon capture has been found in microalgae because of their high photosynthetic capacity and quick growth. The carbon concentration mechanism of many microalgae is heavily reliant on the enzyme carbonic anhydrase (CA), which catalyze the production of bicarbonate from carbon dioxide. In this study, microalgal samples were collected, characterized, and cultured under controlled conditions for their optimal growth of cultures I-IX. The CA activity was investigated using a standard method; the Wilbur-Anderson assay was used to calculate CA activity in microalgal cultures. The comparative study was then used to measure the activity rate of the collected microalgae. Among the tested, culture I, VI, and IX showed a high enzyme activity rate of 4.15, 4.0, and 4.2 µg·mL-1, respectively. To determine the rate of carbon dioxide hydration, the method involved tracking the pH change in a reaction mixture. In addition, genetic analysis facilitates the identification of key genes involved in CA activity and other metabolic processes, which enhance the knowledge of microalgal physiology, and enables genetic engineering efforts in the future studies. Overall, this investigation emphasizes the significance of studying unknown microalgal culture and their potential CA activity for industrial and bio-energy applications.

Synthesis of biocomposites from microalgal peptide incorporated polycaprolactone/ κ- carrageenan nanofibers and their antibacterial and wound healing property.

Antimicrobial peptides (AMPs) are promising novel agents for targeting a wide range of pathogens. In this study, microalgal peptides derived from native microalgae were incorporated into polycaprolactone (PCL) with ƙ-Carrageenan (ƙ-C) forming nanofibers using the electrospinning method. The peptides incorporated in the nanofibers were characterized by fourier infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy (SEM), and contact angle measurement. The results showed that peptides with molecular weights < 10 kDa, when loaded into nanofibers, exhibited lower wettability. The SEM analysis revealed a thin, smooth, interconnected bead-like structures. The antimicrobial activity of the electrospun nanofibers was evaluated through disc diffusion, and minimum inhibitory activity against Escherichia coli (MTTC 443), and Staphylococcus aureus (MTTC 96), resulting in zones of inhibition of 24 ± 0.5 mm and 14 ± 0.5 mm, respectively. The in vitro biocompatibility of the synthesized nanofibers was confirmed using in HEK 293 cell lines with an increased cell viability. Interestingly, the fibers also exhibited a significant wound-healing properties when used in vitro scratch assays. In conclusion, algal peptides incorporated with PCL/ ƙ-C were found to exhibit antimicrobial and biocompatible biomaterials for wound healing applications.

Microalgae mediated bioremediation of polycyclic aromatic hydrocarbons: Strategies, advancement and regulations.

Polycyclic aromatic hydrocarbons (PAHs) are pervasive in the atmosphere and are one of the emerging pollutants that cause harmful effects in living systems. There are some natural and anthropogenic sources that can produce PAHs in an uncontrolled way. Several health hazards associated with PAHs like abnormality in the reproductive system, endocrine system as well as immune system have been explained. The mutagenic or carcinogenic effects of hydrocarbons in living systems including algae, vertebrates and invertebrates have been discussed. For controlling PAHs, biodegradation has been suggested as an effective and eco-friendly process. Microalgae-based biosorption and biodegradation resulted in the removal of toxic contaminants. Microalgae both in unialgal form and in consortium (with bacteria or fungi) performed good results in bioaccumulation and biodegradation. In the present review, we highlighted the general information about the PAHs, conventional versus advanced technology for removal. In addition microalgae based removal and toxicity is discussed. Furthermore this work provides an idea on modern scientific applications like genetic and metabolic engineering, nanomaterials-based technologies, artificial neural network (ANN), machine learning (ML) etc. As rapid and effective methods for bioremediation of PAHs. With several pros and cons, biological treatments using microalgae are found to be better for PAH removal than any other conventional technologies.

Extraction and characterization of Chitosan from Shell of Borassus flabellifer and their antibacterial and antioxidant applications.

Chitosan is a bio-polymer made up of repeating units of N-acetyl glucosamine and glucosamine joined together by (1-4)-glycosidic linkages. Various bioresources have been used to develop bioactive materials that have a wide range of applications in different fields, including industry and medicine. Borassus flabellifer is a well-known source of chitin in the sub-Indian continent and is used in digestion, pharmaceuticals, and other applications. Chitin can be extracted from B. flabellifer fruit shells through demineralization and deproteinization and then converted into chitosan through deacetylation. This study aimed to investigate the biological activity of chitosan extracted from B. flabellifer fruit shells and to analyze its molecular structure using FT-IR analysis. Results showed the presence of NH, OH, and CO stretching, indicating the presence of various functional groups in chitosan. Scanning electron microscopic study revealed the topography of the chitosan. Well-diffusion and MIC tests showed that chitosan exhibited activity against E. coli and S. aureus. The chitosan extract also exhibited potential antioxidant polymer by scavenging free radicals.

Isolation and Characterization of Biosurfactant-Producing Soil Fungus Penicillium sp.

In this study, a fungal species was isolated from rhizospheric soil and identified as Penicillium sp. by ITS sequencing. The Penicillium sp. has been screened for the biosurfactant production, viz., haemolytic activity, oil spreading assay and emulsification index. The biosurfactant from cell-free supernatant was extracted using acid precipitation followed by solvent-solvent extraction. The physiochemical properties of the extracted biosurfactant were analysed using FTIR; the major peaks that show at 1720 cm-1, 1531 cm-1, 1419 cm-1, 1251 cm-1 and 1010 cm-1 correspond to aliphatic chains, sugars and ester carbonyl groups. The fatty acids present in the extracted biosurfactant were analysed using GCMS, in which a molecular mass of 256 and 284 m/z showed the presence of n-hexadecenoic acid and octadecanoic acid respectively which indicate the presence of rhamnolipid, which is a major biosurfactant. The biosurfactant extracted from Penicllium sp. demonstrated antibacterial activity against Escherichia coli and Staphylococcus aureus. In future perspectives, the biosurfactant extracted from the isolated species holds great potential as a broad-spectrum antibacterial agent and could be utilized in various healthcare applications.

State-of-the-art review on the ecotoxicology, health hazards, and economic loss of the impact of microcystins and their ultrastructural cellular changes.

Cyanobacteria are ubiquitously globally present in both freshwater and marine environments. Ample reports have been documented by researchers worldwide for pros and cons of cyanobacterial toxins. The implications of cyanobacterial toxin on health have received much attention in recent decades. Microcystins (MCs) represent the unique class of toxic metabolites produced by cyanobacteria. Although the beneficial aspects of cyanobacterial are numerous, the deleterious effect of MCs overlooked. Several studies on MCs evidently reported that MCs exhibit a plethora of harmful effect on animals, plants, and cell lines. Accordingly, numerous histopathological studies have also found that MCs cause detrimental effects to cells by damaging cellular organelles, including nuclear envelope, Golgi apparatus, endoplasmic reticulum, mitochondria, plastids, flagellum, pilus membrane structures and integrity, vesicle structures, and autolysosomes and autophagosomes. Such ultrastructural cellular damages holistically influence the morphological, biochemical, physiological, and genetic status of the host. Indeed, MCs have also been found to cause the deleterious effect to different animals and plants. Such deleterious effects of MCs have greater impact on agriculture, public health which in turn influences ecotoxicology and economic consequences. The impairments correspond to oxidative stress, organ failure, carcinogenesis, aquaculture loss, with an emphasis for blooms and respective bioaccumulation prospects. The preservation of mortality among life forms is addressed in a critical cellular perspective for multitude benefits. The comprehensive cellular assessment could provide opportunity to develop strategy for therapeutic implications.

A state of the art review on the co-cultivation of microalgae-fungi in wastewater for biofuel production.

The microalgae have a great potential as the fourth generation biofuel feedstock to deal with energy crisis, but the cost of production and biomass harvest are the major hurdles in terms of large scale production and applications. Using filamentous fungi to culture targeted alga for biomass accumulation and eventually harvesting is a sustainable way to mitigate environmental impacts. Microalgal co-culture method could be an alternative to overcome limitations and increase biomass yield and lipid accumulation. It was found to be the high feasibility for the production of biofuels from fungi and microalgae using wastewater. This article aimed to state the synergistic approaches, their culture protocols, harvesting procedure and their potential biotechnological applications. Additionally, algal-fungal consortia could digest cellulosic biomass, potentially reducing operating costs as part of industrial need. As a result of co-cultivation, biofuel production could be economically feasible owing to its excellent ability to treat wastewater and be eco-friendly. The implications of the innovative co-cultivation technology have demonstrated the potential for further development based on the policies that have been supported and implemented.

Functional characterization of maltodextrin glucosidase for maltodextrin and glycogen metabolism in Vibrio vulnificus MO6-24/O.

Glycogen is important for transmission of V. vulnificus undergoing disparate environments of nutrient-rich host and nutrient-limited marine environment. The malZ gene of V. vulnificus encoding a maltodextrin glucosidase was cloned and over-expressed in E. coli to investigate its roles in glycogen/maltodextrin metabolism in the pathogen. The malZ gene encoded a protein with a predicted molecular mass of 70 kDa. The optimal pH and temperature of MalZ was 7.0 and 37 °C, respectively. MalZ hydrolyzed maltodextrin to glucose and maltose most efficiently, while hydrolyzed other substrates such as starch, maltose, ß-cyclomaltodextrin, and glycogen less efficiently. The activity was enhanced greatly by Mn2+. It also exhibited transglycosylation activity toward excessive maltotriose. The malZ knock-out mutant accumulated 2.3-5.6-fold less glycogen than the wild type when excessive maltodextrin or glucose was added to LB medium, while it accumulated more glycogen than the wild type (3.5-fold) in the presence of excessive maltose. Growth and glycogen accumulation of the mutant were retarded most significantly in the M63 minimal medium supplemented with 0.5% maltodextrin. Side chain length distributions of glycogen molecules were varied by the malZ mutation and types of the excessive carbon source. Based on the results, MalZ of V. vulnificus was likely to be involved in maltose/maltodextrin metabolism, thereby balancing synthesis of glycogen and energy generation in the cell. The bacterium seemed to have multiple and unique pathways for glycogen metabolism according to carbon sources.

Current strategies on algae-based biopolymer production and scale-up.

The craving for an alternative to the existing plastic products gives rise to the concept of algae-based bioplastic production, which appears to be excellently biodegradable and cost-effective. The significant assortment of algal biopolymers draws great attention to stop the surge of plastic waste and to mitigate the burning problems of environmental pollution. The polyhydroxyalkanoates (PHA) are naturally-occurring biopolymers found in the form of esters accumulated within a number of microbes, which provides the pillar for several biomolecules. This review summarizes the global scenario as well as the precise technique of algae-based PHA extraction and bioplastic production. In addition, different techniques for valorisation of PHA production, its biodegradability and its commercial applications are also taken into consideration.

Fungal-mediated synthesis of pharmaceutically active silver nanoparticles and anticancer property against A549 cells through apoptosis.

Generally, fungi have the ability to secrete large amounts of secondary metabolites which have the ability to reduce metal ions to metallic nanoparticles. In this report, silver nanoparticles (AgNPs) were synthesized by using an endophytic fungus isolated from the medicinal plant, Catharanthus roseus (Linn.). The endophytic fungus was identified as Botryosphaeria rhodina based on the ITS sequencing. The synthesized AgNPs were characterized by adopting various high-throughput techniques, scanning electron microscopy (SEM) equipped with energy dispersive X-ray analysis (EDAX), high-resolution transmission electron microscopy (HR-TEM) and UV-Visible spectrophotometer. In vitro anticancer efficacy of AgNPs was tested on A-549 cells. The synthesized AgNPs were effective in scavenging free radicals and induced hallmarks of apoptosis including nuclear and DNA fragmentation in lung (A549) cancer cell lines under in vitro conditions. The results suggested that the natural biomolecules in the endophytic fungi incorporated into the nanoparticles could be responsible for the synergetic cytotoxic activity against cancer cells. The AgNPs were found to have cytotoxicity IC50 of 40 µg/mL against A549 cells. To the best our knowledge, this is the first report demonstrating that AgNPs from Botryosphaeria rhodina could be able to induce apoptosis in various types of cancer cells as a novel strategy for cancer treatment.

Solution plasma mediated formation of low molecular weight chitosan and its application as a biomaterial.

Low molecular weight (LMW) chitosan has been a great attention in bio-molecular chemistry, medicine, and drug delivery system in particular. Depolymerization of high molecular weight (HMW) chitosan to LMW chitosan was achieved by solution plasma process (SPP) without affecting its chemical structures. Chitosan in solution was depolymerized by discharging plasma at 800 V with 35 kHz for various times (15-120 min). Gel permeation chromatography analysis revealed that molecular weight of chitosan decreased from 3.0 × 105 Da to 7.8 × 103 Da in 30 min plasma treatment, and further to 4.6 × 103 Da in 90 min. Dynamic light scattering and zeta potential studies confirmed formation of chitosan nano-aggregates. Interestingly, the LMW chitosan samples showed antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans with minimal inhibitory concentration of 80-1200 µg·mL-1. They also exhibited an excellent antioxidant activity (58-75%) and swelling ratio of 0.2-2.0 mg·mg-1. LMW chitosan was likely to have potential for sustainable usage as carrier molecules, biomaterials, and biomedical applications.

Antioxidant potentials of nanoceria synthesized by solution plasma process and its biocompatibility study.

Nanoceria were synthesized by discharging plasma at 800 V with a frequency of 30 kHz for 0-25 min using a pulsed unipolar power supply into solutions containing 1 or 2 mM of Ce(NO3)2. UV-Vis spectroscopy showed a characteristic absorbance maxima at 304-320 nm for the nanoceria with increase in the intensity of the peaks as the concentration of Ce(NO3)2 increased. The peaks exhibited transition red shift due to nanoceria formation. High resolution transmission electron microscopy revealed that spherical nanoparticles with an average size of 7.0 ±â€¯0.2 nm were formed by discharging plasma for 15 min. The nanoceria showed excellent pH dependent antioxidant properties in hydroxyl and superoxide anion radical scavenging assays. Effect of the nanoceria on cell viability in vitro and inhibition of reactive oxygen species (ROS) by the nanoceria were examined using HeLa cell lines. As the results, no toxic effect was found up to 1600 µg mL-1 of nanoceria and they had an effective antioxidant property. Therefore, the nanoceria synthesized by one-step solution plasma process without employing hazardous chemicals have potential for utilization as antioxidant biomaterials and sustained release in the stream to scavenge ROS in the modern medicine.

An evidence of C16 fatty acid methyl esters extracted from microalga for effective antimicrobial and antioxidant property.

Fatty acid methyl esters (FAME) derived from lipids of microalgae is known to have wide bio-functional materials including antimicrobials. FAME is an ideal super-curator and superior anti-pathogenic. The present study evaluated the efficiency of FAME extracted from microalgae Scenedesmus intermedius as an antimicrobial agent against Gram positive (Staphylococcus aureus, Streptococcus mutans, and Bacillus cereus) Gram negative (Escherichia coli and Pseudomonas aeruginosa) bacteria and Fungi (Aspergillus parasiticus and Candida albicans). The minimal inhibitory concentration (MIC) for the gram negative bacteria was determined as 12-24 µg mL-1, whereas MIC for gram positive bacteria was 24-48 µg mL-1. MIC for the fungi was as high as 60-192 µg mL-1. The FAME profiles determined by gas chromatography showed 18 methyl esters. Among them, pharmacologically active FAME such as palmitic acid methyl ester (C16:0) was detected at high percentage (23.08%), which accounted for the bioactivity. FAME obtained in this study exhibited a strong antimicrobial activity at the lowest MIC than those of recent reports. This result clearly indicated that FAME of S. intermedius has a strong antimicrobial and antioxidant property and that could be used as an effective resource against microbial diseases.

The facile synthesis of chitosan-based silver nano-biocomposites via a solution plasma process and their potential antimicrobial efficacy.

Silver nanoparticles (AgNPs) were synthesized in a chitosan matrix with varying AgNO3 (1, 3, 5 mM) and chitosan (1, 3%) concentrations via the one-step solution plasma process (SPP). Plasma was discharged for 3 min in the AgNO3 and chitosan solutions using unipolar power at 800 V with a frequency of 30 kHz. Fibrous 3D scaffolds were prepared by lyophilizing the nano-biocomposite solutions, and they were stabilized via cross-linking with UV irradiation. UV-Vis spectroscopy showed strong peaks with maximal absorbance at 415-440 nm, indicating the formation of AgNPs in the chitosan with an increase in peak height as the concentration of the precursor, AgNO3, increased. The chemical association between AgNPs and chitosan was confirmed using Fourier transform infrared spectroscopy (FTIR). The scaffolds had a micro-porous structure with pore diameters in the range of 5.8-157.0 µm, and a transmission electron microscopy (TEM) analysis revealed that spherical shaped AgNPs with diameters in the range of 2.5-27.6 nm were well-dispersed in the biocomposites. The nano-biocomposites had a broad spectrum of antimicrobial activity against various pathogens with minimal inhibition concentrations of 0.68-2.71 and 2.71-10.80 µg mL(-1) for bacteria and fungi, respectively. These are the lowest concentrations achieved by nano-biocomposites reported thus far. The SPP was shown to be a facile, effective, and eco-friendly method of synthesizing nano-biocomposites for biomedical applications.