RESUMO
The occurrence of Nyssomyia intermedia s.lat. in the state of Paraná, Brazil, has been registered by several authors; however, studies have identified this taxon as belonging, in Paraná, to Nyssomyia neivai (Pinto). During captures with traps in a hen-house and forested areas, from November 2005 to October 2006, in Adrianópolis, Morretes and Pontal do Paraná, situated in the Atlantic forest domain, Paraná state, seven females of Nyssomyia intermedia s.str. (Lutz & Neiva) were collected together with other 14 sand floy species. Thus the occurrence of N. intermedia on the coast and in areas of Atlantic forest in Paraná is confirmed.
Assuntos
Psychodidae , Animais , Brasil , Demografia , FemininoRESUMO
Classic and molecular (polymerase chain reaction--PCR) techniques were used to diagnose American cutaneous leishmaniasis in 149 dogs from an area in the northwest of Paraná State, Brazil, where an American cutaneous leishmaniasis outbreak occurred in 2002. The results were compared to a set of previously obtained results. Twenty-five dogs had positive indirect immunofluorescence (IIF) (titers > or = 40), including two animals with suggestive lesions. The percentage of dogs with positive IIF was similar to that found in a previous study. The cultures of the lesion, blood and bone marrow were negative for Leishmania. A direct search for the parasite in the lesions proved negative, although PCR tests were positive. The PCR did not detect the DNA of Leishmania (Viannia) in the blood, even for those that had positive PCR in a previous study. The follow up of the 27 dogs showed that the majority of them had maintained the same levels of antibodies that had been detected previously. There was a reduction in the number of dogs with lesions, probably due to the transmission control measures that were adopted after the outbreak.
Assuntos
Reservatórios de Doenças/veterinária , Doenças do Cão/diagnóstico , Leishmania braziliensis , Leishmaniose Cutânea/veterinária , Animais , Anticorpos Antiprotozoários/sangue , Anticorpos Antiprotozoários/genética , Medula Óssea/parasitologia , Medula Óssea/patologia , Brasil/epidemiologia , Meios de Cultura , DNA de Protozoário/sangue , DNA de Protozoário/genética , Surtos de Doenças/veterinária , Reservatórios de Doenças/parasitologia , Reservatórios de Doenças/estatística & dados numéricos , Doenças do Cão/epidemiologia , Doenças do Cão/parasitologia , Cães , Feminino , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Leishmania braziliensis/genética , Leishmania braziliensis/imunologia , Leishmania braziliensis/isolamento & purificação , Leishmaniose Cutânea/sangue , Leishmaniose Cutânea/epidemiologia , Leishmaniose Cutânea/genética , Masculino , Reação em Cadeia da Polimerase/veterinária , População Rural , Úlcera Cutânea/genética , Úlcera Cutânea/patologia , Úlcera Cutânea/veterinária , Fatores de TempoRESUMO
The results from sandfly collections in 10 municipalities in Paraná State, Brazil are reported. The captures were done using Falcão traps in homes, domestic animal shelters and forested areas, from 1999 to 2002. A total of 13,653 sandflies were collected from 10 species of the genera Brumptomyia, Expapillata, Evandromyia, Migonemyia, Pintomyia, Nyssomyia and Psathyromyia. The species Nyssomyia neivai was predominant in five municipalities. N. whitmani predominated in the other five, in greater numbers than for N. neivai in the first five municipalities. High frequencies of sand flies were found in forests, homes, pigpens and henhouses. Investigations on the participation of domestic animals and phlebotomine fauna in the epidemiology of tegumentary leishmaniasis should be routine in health surveillance, especially where this disease is endemic.
Assuntos
Insetos Vetores/classificação , Leishmaniose Cutânea/epidemiologia , Phlebotomus/classificação , Animais , Animais Domésticos , Brasil/epidemiologia , Feminino , Humanos , Leishmaniose Cutânea/transmissão , Masculino , Densidade DemográficaRESUMO
American cutaneous leishmaniasis (ACL) is an endemic disease in northern Paraná State, where it affects humans and dogs. This study aimed to verify the occurrence of the canine disease in ACL endemic areas. From September 1999 to July 2002, dogs were investigated in 7 rural areas of 5 municipalities where autochthonous human cases of ACL had been reported. Parasitological and serological methods were used. Fourteen of 67 dogs studied (20.9%) had lesions suggestive of ACL, of which 3 (21.4%) were infected with Leishmania sp. Indirect immunofluorescence for Leishmania (Viannia) braziliensis was positive in 37 (55.2%) of 67 dogs. The results showed that human and canine ACL occur simultaneously and indicate the need for additional studies to elucidate the role of dogs in the ACL transmission cycle in the area studied.
Assuntos
Anticorpos Antiprotozoários/sangue , Doenças do Cão/epidemiologia , Doenças Endêmicas , Leishmania/imunologia , Leishmaniose Cutânea/veterinária , Animais , Brasil/epidemiologia , Doenças do Cão/diagnóstico , Doenças do Cão/parasitologia , Cães , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Leishmaniose Cutânea/diagnóstico , Leishmaniose Cutânea/epidemiologia , Masculino , População RuralRESUMO
Phlebotomine sandfly captures were performed in the domicile, domestic animal shelters, and peridomicile before and after rearrangement, cleaning, and disinsectization. Phlebotomine sandflies were captured with Falcão traps from October 1998 to April 2000 in the Recanto Marista district, Doutor Camargo municipality, Paraná State. The results were compared with those from October 1996 to September 1997. In this survey, 122,616 phlebotomine sandflies were captured using 10 traps, as compared to 157,983 in the previous period, with 6 traps. Lutzomyia neivai predominated in both periods. During the former period an hourly average of 1,641.9 phlebotomine sandflies were captured as compared to 806.7 in the latter. The results with four traps installed in the same locations shows that the hourly average was 1,318.8 in the former period and 156.0 in the latter. The large reduction in the number of phlebotomine sandflies captured in the second period may be due to the impact of alterations in the environment and the disinsectization of the buildings after the first period.