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1.
Anaesth Intensive Care ; 42(4): 480-6, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24967763

RESUMO

Spinal ultrasonography is a promising aid for epidural insertion. We aimed to determine the learning curve of spinal ultrasonography tasks and the number of training scans required to reach competency after undergoing standardised step-wise teaching. Trainees were required to complete a minimum of 60 assessed scans on selected non-pregnant models following attendance at two training sessions, with feedback from an expert after each scan. Learning curves were plotted using the non-risk cumulative summation technique and an acceptable failure rate of 20%. Five trainees completed between 65 and 75 scans each. All trainees were competent at identifying a randomly assigned intervertebral space after a median of five scans (range one to nine) and at measuring the depth from skin to the posterior complex after a median of 10 scans (range 1 to 42). Two trainees were competent at marking an ideal needle insertion point after 55 scans, while three trainees did not attain competency. All trainees were competent after 60 scans if the tolerance was changed from five to eight millimetre for marking the needle insertion point. The average time taken to complete a scan was 163 seconds. Our study showed that after a standardised educational intervention, anaesthetic trainees are able to identify a lumbar interlaminar space easily and can measure the depth to the posterior complex after a reasonable number of additional practice scans, but experienced difficulty accurately marking the needle insertion point whilst using spinal ultrasonography. We confirmed that it was hard to achieve competency in all aspects of spinal ultrasonography, based on assessment using our predefined competency criteria.


Assuntos
Raquianestesia/métodos , Anestesiologia/educação , Competência Clínica/estatística & dados numéricos , Avaliação Educacional/métodos , Curva de Aprendizado , Ultrassonografia de Intervenção/métodos , Adulto , Raquianestesia/estatística & dados numéricos , Anestesiologia/métodos , Anestesiologia/estatística & dados numéricos , Austrália , Avaliação Educacional/estatística & dados numéricos , Feminino , Humanos , Estudos Prospectivos , Ultrassonografia de Intervenção/estatística & dados numéricos , Adulto Jovem
2.
Histopathology ; 31(3): 277-83, 1997 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-9354900

RESUMO

AIMS: Intestinal metaplasia (IM) has been implicated in the pathogenesis of gastro-oesophageal carcinoma, but because of its common occurrence, its specificity for use in cancer surveillance is low. IM subtypes characterized by mucin phenotype have been studied to try and improve specificity. METHODS AND RESULTS: On balance, type III IM seems the most promising for use in gastric cancer surveillance. The situation is problematic at the gastro-oesophageal junction where the normal occurrence of acidic mucins raises doubt on the value of subtyping. High iron diamine-Alcian blue combination (HID-AB) is commonly used for IM subtyping, but its potential toxicity and long staining period (up to 24 hours) precludes widespread clinical use. This study has compared the sulphomucin staining ability of Gomori's aldehyde fuchsin-Alcian blue combination (GAF-AB) against HID-AB for identifying and subtyping IM in gastric and oesophageal biopsies. CONCLUSIONS: Compared to HID-AB, a sensitivity of 85%, a specificity of 100% and a staining time of less than 30 minutes, shows this stain to be a simple and effective technique for identifying and subtyping IM in routine laboratories.


Assuntos
Esôfago/patologia , Intestinos/patologia , Metaplasia/classificação , Coloração e Rotulagem/métodos , Estômago/patologia , Biópsia , Estudos de Avaliação como Assunto , Histocitoquímica/métodos , Humanos , Metaplasia/diagnóstico , Corantes de Rosanilina , Sensibilidade e Especificidade
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