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1.
Front Oncol ; 14: 1320714, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38487727

RESUMO

Objective: We aimed to investigate the value of contrast-enhanced ultrasound (CEUS) in the preoperative prediction of the histological grades and molecular subtypes of breast cancer. Methods: A total of 183 patients with pathologically confirmed breast cancer were included. Contrast enhancement patterns and quantitative parameters were compared in different groups. The receiver operating characteristic (ROC) curve was used to analyze the efficacy of CEUS in the preoperative prediction of pathological characteristics, including histologic grade and molecular subtypes. Results: Heterogeneous enhancement, perfusion defects, and peripheral radial vessels were mostly observed in higher histologic grade (grade III) breast cancer. Heterogeneous enhancement and perfusion defect were the most effective indicators for grade III breast cancer, with the areas under the ROC curve of 0.768 and 0.756, respectively. There were significant differences in the enhancement intensity, post-enhanced margin, perfusion defects, and peripheral radial vessel among the different molecular subtypes of breast cancer (all P < 0.01). Perfusion defects and clear edge after enhancement were the best qualitative criteria for the diagnosis of HER-2 overexpressed and triple-negative breast cancers, and the corresponding areas under the ROC curves were 0.804 and 0.905, respectively. There were significant differences in PE, WiR, WiPI, and WiWoAUC between grade III vs grade I and II breast cancer (P < 0.05). PE, WiR, WiPI, and WiWoAUC had good efficiency in the diagnosis of high-histologic-grade breast cancer. PE had the highest diagnostic efficiency in Luminal A, while WiPI had the highest diagnostic efficiency in Luminal B subtype breast cancer, and the areas under the ROC curve were 0.825 and 0.838, respectively. WiWoAUC and WiR were the most accurate parameters for assessing triple-negative subtype breast cancers, and the areas under the curve were 0.932 and 0.922, respectively. Conclusion: Qualitative and quantitative perfusion analysis of contrast-enhanced ultrasound may be useful in the non-invasive prediction of the histological grade and molecular subtypes of breast cancers.

2.
Anatol J Cardiol ; 2024 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-38445622

RESUMO

BACKGROUND: Empagliflozin (EMPA) demonstrates cardioprotective effects on the patients with heart failure, but its effects in cardiorenal syndrome (CRS) remain unspecified. The purpose of the exploratory study was to investigate the effect of EMPA on patients with type 2 CRS and type 2 diabetes mellitus (DM). METHODS: This study was a randomized trial of patients with type 2 CRS and DM done between December 2020 and January 2022. Patients were randomly allocated to the control group and the EMPA group using EMPA as an add-on treatment. Serum interleukin 6 (IL-6), janus kinase 2 (JAK-2), and signal transducer and activator of transcription 3 (STAT-3) concentrations were measured in 102 patients with CRS and healthy individuals without any disease using enzyme-linked immunosorbent assay before and after treatment. The evaluation of renal function was measured by immunoturbidimetry, and cardiac function was estimated by doppler echocardiography. Rates of adverse events and major adverse cardiac events (MACE) were documented. RESULTS: The results showed that EMPA decreased the level of IL-6 but increased the level of JAK-2 and STAT-3 in patients. Additionally, the results suggest EMPA significantly reduced the incidence of MACE compared to the control group, while the rate of adverse events did not significantly differed. CONCLUSIONS: Our study suggested that the cardiorenal benefits conferred by EMPA might be driven by anti-inflammatory effects, cooperated with the activation of JAK2/STAT3 signaling pathways, leading to modest short-term improvements in patients with type 2 CRS. The overall safety and low complication make EMPA a significant choice for clinical application.

3.
J Org Chem ; 87(22): 15661-15669, 2022 11 18.
Artigo em Inglês | MEDLINE | ID: mdl-36317696

RESUMO

By employing Cu(CH3CN)4PF6 as the catalyst and tert-butyl hydroperoxide as the oxidant, we realized a three-component radical selenosulfonation of substituted maleimides, sulfonyl hydrazides, and diphenyl diselenides, providing a series of 3,4-selenosulfonylated succinimides in moderate to good yields. This reaction features broad substrate scopes, high functional-group tolerability, and feasibility of gram-scale synthesis, enabling one-step construction of C-SO2 and C-Se bonds under mild reaction conditions. Preliminary mechanistic studies support the free-radical-induced pathway.


Assuntos
Cobre , Iodo , Cobre/química , Maleimidas , Estrutura Molecular , Hidrazinas/química , Catálise
4.
Cell Microbiol ; 23(12): e13391, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34482605

RESUMO

Biofilm plays an important role in fungal multidrug resistance (MDR). Our previous studies showed that BSC2 is involved in resistance to amphotericin B (AMB) through antioxidation in Saccharomyces cerevisiae. In this study, the overexpression of BSC2 and IRC23 induced strong MDR in S. cerevisiae. BSC2-overexpression affected cellular flocculation, cell surface hydrophobicity, biofilm formation and invasive growth. However, it failed to induce caspofungin (CAS) resistance and affect the invasive growth in FLO mutant strains (FLO11Δ, FLO1Δ, FLO8Δ and TUP1Δ). Furthermore, the overexpression of BSC2 compensated for chitin synthesis defects to maintain the cell wall integrity and significantly reduced the cell morphology abnormality induced by CAS. However, it could not repair the cell wall damage caused by CAS in the FLO mutant strains. Although BSC2 overexpression increased the level of mannose in the cell wall, DPM1 overexpression in both BY4741 and bsc2∆ could confer resistance to CAS and AMB. In addition, BSC2 overexpression significantly increased the mRNA expression of FLO11, FLO1, FLO8 and TUP1. BSC2 may function as a regulator of FLO genes and be involved in cell wall integrity in yeast. Taken together, our data demonstrate that BSC2 induces MDR in a FLO pathway-dependent manner via contributing to the formation of biofilms in S. cerevisiae. TAKE AWAYS: Overexpression of BSC2 induced strong MDR in S. cerevisiae. BSC2 affected cellular flocculation, CSH, biofilm formation and invasive growth. BSC2 could not repair the cell wall damage caused by CAS in the FLO mutants. BSC2 may function as a regulator of FLO genes to maintain cell wall integrity. BSC2 promotes biofilm formation in a FLO pathway-dependent manner to induce MDR.


Assuntos
Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae , Biofilmes , Resistência a Múltiplos Medicamentos , Floculação , Glicoproteínas de Membrana , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética
5.
J Org Chem ; 84(17): 11032-11041, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31333030

RESUMO

A regioselective synthesis of polysubstituted dihydropyrazoles and pyrazoles through an iodine-catalyzed oxidative cyclization strategy of aldehyde hydrazones with electron-deficient olefins is described. The protocol adopts very mild reaction conditions and provides desirable yields. The reaction is supposed to proceed via a cascade C-H functionalization, C-N bond formation, and oxidation sequential processes. The overall simplicity and regioselectivity of the catalytic system make this approach a valuable and step-economical tool to construct a C-C bond for the synthesis of Mefenpyr-Diethyl.

6.
IUBMB Life ; 70(8): 753-762, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-30092116

RESUMO

As a lipin family founding member, lipin1 exerts dual functions as a phosphatidate phosphatase enzyme and/or a co-transcriptional regulator in lipid metabolism. In fact, it is also involved in many other cell processes. In this study, we utilized pull down assay coupled with mass spectrometry (MS) to unravel protein-protein interaction networks of lipin1 in 293T human embryonic kidney cells. Pull-down assay on the Ni2+ -chelating column was used to isolate lipin1 complexes from 293T cells transfected with 6-His tagged lipin1. The lipin1 complexes were analyzed on Q Exactive mass spectrometer. A total of 30 proteins were identified from label free quantitation of the MS data by Proteome Discoverer platform. The physical interaction between lipin1 and eEF1A1 was further affirmed in 293T cells transfected with 6-His tagged lipin1 and hepatocyte SMMC7721 cells by protein immunoprecipitation and immunofluorescence microscopy. Lipin1 also interacted with HIST1H2BK, which was confirmed in SMMC7721 cells by protein immunoprecipitation. Our proteomic analysis implicated lipin1 in novel roles in various cellular processes. © 2018 IUBMB Life, 70(8):753-762, 2018.


Assuntos
Histonas/genética , Fator 1 de Elongação de Peptídeos/genética , Fosfatidato Fosfatase/genética , Proteômica/métodos , Células HEK293 , Hepatócitos/metabolismo , Humanos , Imunoprecipitação , Metabolismo dos Lipídeos/genética , Fígado/metabolismo , Espectrometria de Massas , Mapas de Interação de Proteínas/genética
7.
FEMS Microbiol Lett ; 365(18)2018 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-30085078

RESUMO

Cadmium has been shown to be an important environmental pollutant. Our previous studies have shown that the regulation of OLE1 in the synthesis of unsaturated fatty acids may act as a positive feedback mechanism to help yeast cells counter the lipid peroxidation and cytoplasmic membrane damage induced by cadmium. However, the involvement of OLE1 in cadmium-induced oxidative stress is still unclear. In this study, we explored the effects of OLE1 on cadmium-induced oxidative stress in yeast. Different from ascorbic acid, the overexpression of OLE1 did not affect the activities of superoxide dismutase, catalase and glutathione peroxidase. Although OLE1 overexpression induced an increase in GSH levels, the anti-oxidative mechanism of OLE1 was GSH1 independent. On the other hand, similar to ascorbic acid, OLE1 overexpression significantly reduced the levels of superoxide radical, carbonyl protein and lipid peroxidation. Additionally, overexpression of OLE1 effectively prevented cell membrane damage induced by cadmium. OLE1 could also reduce the cytotoxicities of other heavy metals stress, including copper, tri- and hexavalent chromium. Thus, our results indicate that overexpression of OLE1 reduces oxidative stress induced by cadmium possibly through the inhibition of lipid peroxidation and protection of the cytoplasmic membrane from damage in yeast cells. Furthermore, we determined that the anti-oxidative effect of OLE1 is independent of antioxidant enzymes and GSH1.


Assuntos
Cádmio/toxicidade , Peroxidação de Lipídeos , Estresse Oxidativo , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/enzimologia , Estearoil-CoA Dessaturase/metabolismo , Poluentes Ambientais/toxicidade , Expressão Gênica , Proteínas de Membrana/metabolismo , Estearoil-CoA Dessaturase/genética , Superóxidos/metabolismo
8.
Vet Parasitol ; 204(3-4): 323-9, 2014 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-24974121

RESUMO

Octadecanoic acid-3,4-tetrahydrofuran diester, isolated from neem (Azadirachta indica) oil, exhibited potent acaricidal activity against Sarcoptes scabiei var. cuniculi. In this paper, the acaricidal mechanism of octadecanoic acid-3,4-tetrahydrofuran diester against Sarcoptes scabiei var. cuniculi was evaluated based on pathologic histology and enzyme activities. The results showed that after compound treatment for 24h at a concentration of 20mg/mL, the lesions of mites were prominent under transmission electron microscopy. The lesions consisted of the lysis of dermis cell membranes and cell nuclear membranes, mitochondrial morphological abnormalities, the drop of spinal disorders, and mitochondrial vacuolization. The activity of superoxide dismutase (SOD), peroxidase (POD), glutathione-s-transferases (GSTs), and Ca(2+)-ATPase of mites significantly changed after treatment with octadecanoic acid-3,4-tetrahydrofuran diester compared with the control group. The activities of SOD, POD, and Ca(2+)-ATPase were significantly suppressed, whereas that of GSTs was activated. These results indicated that the mechanism of the acaricidal activity of octadecanoic acid-3,4-tetrahydrofuran diester was mainly achieved through interference with the energy metabolism of mites, thus resulting in insect death.


Assuntos
Acaricidas/farmacologia , Azadirachta/química , Sarcoptes scabiei/efeitos dos fármacos , Escabiose/tratamento farmacológico , Ácidos Esteáricos/farmacologia , Acaricidas/isolamento & purificação , Animais , ATPases Transportadoras de Cálcio/metabolismo , Glutationa Transferase/metabolismo , Microscopia Eletrônica de Transmissão , Peroxidase/metabolismo , Sarcoptes scabiei/enzimologia , Sarcoptes scabiei/ultraestrutura , Ácidos Esteáricos/isolamento & purificação , Superóxido Dismutase/metabolismo
9.
Planta Med ; 79(9): 792-6, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23670620

RESUMO

Three new xanthones, 22,23-dihydroxydihydrogambogenic acid (1), 12-hydroxygambogefic acid A (2), and hanburixanthone (3), along with thirteen known compounds were isolated from the resin of Garcinia hanburyi. The structures of the new compounds were determined by detailed analysis of 1D and 2D NMR spectra and by comparison with related model compounds. All compounds were tested for their cytotoxicities against A549, HCT116, and MDA-MB-231, and most of them showed significant effects on the cell lines.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Garcinia/química , Resinas Vegetais/química , Xantonas/química , Xantonas/farmacologia , Antineoplásicos Fitogênicos/química , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Células HCT116/efeitos dos fármacos , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Xantonas/análise , Xantonas/isolamento & purificação
10.
Environ Toxicol Pharmacol ; 35(2): 240-6, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23353547

RESUMO

Neem (Azadirachta indica), popularly known as traditional medicine is a native plant in India. Neem oil is a vegetable oil derived from seeds or fruits of the neem tree through pressing or solvent extraction, and largely used in popular medicine to have antifungal, antibacterial, antimalarial, antiparasitic, anti-inflammatory, as well as immunemodulatory properties in different animal species. In the present study, acute and 28-day subacute toxicity tests were carried out. In the acute toxicity test, the LD50 values of neem oil were found to be 31.95g/kg. The subacute treatment with neem oil failed to change body weight gain, food and water consumption. Serum biochemistry analysis showed no significant differences in any of the parameters examined under the dose of 1600mg/kg/day. Histopathological exams showed that the target organs of neem oil were testicle, liver and kidneys up to the dose of 1600mg/kg/day.


Assuntos
Azadirachta/toxicidade , Glicerídeos/toxicidade , Terpenos/toxicidade , Animais , Azadirachta/química , Peso Corporal/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Ingestão de Alimentos/efeitos dos fármacos , Feminino , Glicerídeos/farmacocinética , Índia , Rim/efeitos dos fármacos , Dose Letal Mediana , Fígado/efeitos dos fármacos , Masculino , Camundongos , Plantas Medicinais/química , Sementes/química , Terpenos/farmacocinética , Testículo/efeitos dos fármacos , Distribuição Tecidual , Testes de Toxicidade Aguda , Testes de Toxicidade Subaguda
11.
Fitoterapia ; 83(8): 1548-52, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22981505

RESUMO

Three new xanthones, garcinolic acid (1), 10α-ethoxy-9,10-dihydromorellic acid (2), and 10α-ethoxy-9,10-dihydrogambogenic acid (3), along with six known compounds were isolated from the resin of Garcinia hanburyi. These compounds were tested for their cytotoxicities against A549, HCT116, SK-BR-3 and HepG2, and showed high inhibitory effects on the cell lines.


Assuntos
Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Garcinia/química , Resinas Vegetais/química , Xantonas/química , Linhagem Celular Tumoral , Humanos , Estrutura Molecular
12.
Acta Crystallogr Sect E Struct Rep Online ; 68(Pt 5): o1420, 2012 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-22590305

RESUMO

The title compound, C(12)H(14)O(6), a substituted isophthalic acid monoester which was isolated from the lichen Thamnolia vermicularis var. subuliformis, displays intra-molecular carbox-yl-meth-oxy O-H⋯O and hy-droxy-carboxyl O-H⋯O hydrogen-bonding inter-actions. The terminal methyl group of the ethyl ester is disordered over two sets of sites with occupancies of 0.599 (19) and 0.401 (19).

13.
Exp Parasitol ; 130(4): 475-7, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22349080

RESUMO

The petroleum ether extract of neem oil and its four fractions separated by column chromatography was diluted at different concentrations with liquid paraffin. The acaricidal bioassay was conducted using a dipping method. The results indicated that the median lethal concentration (LC50) of the petroleum ether extract (at the concentration of 500.0ml/l) was 70.9ml/l, 24h after treatment. At concentrations of 500.0, 250.0, 125.0, 62.5 and 31.2ml/l, the median lethal times (LT50) of the petroleum ether extract were 8.7, 8.8, 10.8, 11.5 and 13.1h, respectively. Thin-layer chromatography (TLC) showed that the petroleum ether extract of neem oil separated into four fractions (F1-F4). Acaricidal activity of 68.3% and 100.0% in the F2 and F4 was confirmed. These results suggest that petroleum ether extracts of neem oil and its four fractions possess useful acaricidal activity in vitro.


Assuntos
Acaricidas , Azadirachta/química , Glicerídeos/química , Sarcoptes scabiei , Terpenos/química , Alcanos , Animais , Cromatografia em Gel/métodos , Larva , Probabilidade , Coelhos , Análise de Regressão , Escabiose/parasitologia , Escabiose/veterinária
14.
Nat Prod Res ; 26(9): 859-64, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22007685

RESUMO

A new lignan (1), named armatumin, along with 25 known compounds, was isolated from Zanthoxylum armatum. The structure of compound 1 was elucidated using spectroscopic methods. Compounds 7, 8, 11, 13, 16, 20 and 21 were first isolated from Z. armatum and compounds 14 and 19 were isolated for the first time from Rutaceae.


Assuntos
Lignanas/isolamento & purificação , Zanthoxylum/química , Espectroscopia de Ressonância Magnética , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria Infravermelho , Espectrofotometria Ultravioleta
15.
Fitoterapia ; 82(3): 347-51, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21059381

RESUMO

Zanthoxylum armatum DC. is a traditional Chinese medicine that is prescribed to alleviate pain and treat inflammatory disorders. This species is distributed mainly in the southeast and southwest regions of China. In the present study, we found that ethyl acetate fraction of ethanolic extract of Z. armatum could significantly decrease acetic acid-induced writhing numbers, and suppress formalin induced licking times in the first phase at the highest dose and in the second phase at all tested doses. This observation revealed that Z. armatum extract possessed powerful antinociceptive activity. The mechanisms of the antinociceptive effect might be mainly involved in the periphery inflammatory analgesic. In addition, the ethyl acetate fraction also inhibited xylene-induced ear swelling in a dose-dependent manner in mice. Eight lignans [eudesmin, horsfieldin, fargesin, kobusin, sesamin, asarinin, planispine A, and pinoresinol-di-3,3-dimethylallyl] were identified as major components of the ethyl acetate fraction. Considering related studies reporting the anti-inflammatory activity for the identified lignans, lignan might be responsible for its anti-inflammatory activity. Our results confirm that the traditional use of Z. armatum in the treatment of inflammation and pain is warranted.


Assuntos
Analgésicos/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Inflamação/tratamento farmacológico , Lignanas/uso terapêutico , Dor/tratamento farmacológico , Fitoterapia , Zanthoxylum/química , Ácido Acético , Analgésicos/farmacologia , Animais , Anti-Inflamatórios/farmacologia , Comportamento Animal/efeitos dos fármacos , Relação Dose-Resposta a Droga , Orelha , Edema/tratamento farmacológico , Formaldeído , Lignanas/análise , Lignanas/farmacologia , Camundongos , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Raízes de Plantas , Caules de Planta
16.
Environ Sci Pollut Res Int ; 19(7): 2690-6, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22293907

RESUMO

PURPOSE: Feathers are one of the most abundant bioresources. They are discarded as waste in most cases and could cause environmental pollution. On the other hand, keratin constituted by amino acids is the main component of feathers. In this article, we reported on biorefined feathers and integrants and application of degraded products. MATERIALS AND METHODS: The fermentation of whole chicken feathers with Stenotrophomonas maltophilia DHHJ in a scale-up of a 5-L bioreactor was investigated in this article. The fermentation process was controlled at 0.08 MPa pressure, 2.5 L/min airflow, and 300 rpm as 100% oxygen saturation level, 40°C, and pH 7.8. RESULTS: Feathers were almost completely degraded in the tested fermentation reaction with the following conditions: 80 g of whole feathers in 3 L fermentation broth for 72 h, seed age of 16 h, 100 mL inoculation amount, and 50% oxygen saturation level. The degraded products contain 397.1 mg/L soluble protein that has mass weight ranging from 10 to 160 kD, 336.9 mg/L amino acids, and many kinds of metal ions. The fermentation broth was evaluated as leaf fertilizer and found to increase plant growth to 82% or 66% for two- or fourfold dilutions, respectively. In addition, in a hair care assay, the broth showed a hair protective function by increasing weight, flexibility, and strength of the treated hair. CONCLUSIONS: The whole feathers were degraded completely by S. maltophilia DHHJ. The degraded product includes many factors to life, such as peptides, amino acids, and mineral elements. It could be applied as leaf fertilizer and hair care product.


Assuntos
Plumas , Fermentação , Eliminação de Resíduos , Stenotrophomonas maltophilia/metabolismo , Matadouros , Animais , Conservação de Recursos Energéticos , Poluentes Ambientais , Preparações para Cabelo , Humanos , Resíduos Industriais , Desenvolvimento Vegetal , Reciclagem/métodos , Fatores de Tempo
17.
Zhongguo Zhong Yao Za Zhi ; 29(3): 225-8, 2004 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-15706845

RESUMO

OBJECTIVE: To study the conditions and parameters of purifying total flavonoids from seed residue of H. rhamnoides. METHOD: Absorption capacity of three resins for total flavonoids was compared. With the yield and purity as indexes, the process of absorbing and purifying total flavonoids from seed residue with D101 macroporous resin absorbent was selected by orthogonal design. RESULT: The D101 resin was the best of the three resins and its absorption capacity was determined to be 63.00 mg x g(-1) wet resin. The optimum process condition was 30% ethanol as eluting solvent, wet resin vs crude herbs: 2:1, diameter vs height: 1:10, eluting solvent vs crude herbs: 10:1, absorbing time for 3 h. The yield of total flavonoids from seeds residue of H. rhamnoides by this process was 2.39%, and the purity reached 64.81%. CONCLUSION: The process is simple and convenient and the regeneration of resin is easy. So this method of purification is advisable.


Assuntos
Flavonoides/isolamento & purificação , Hippophae/química , Plantas Medicinais/química , Resinas Sintéticas , Tecnologia Farmacêutica/métodos , Etanol , Sementes/química
18.
Zhong Yao Cai ; 26(10): 735-7, 2003 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-14768393

RESUMO

OBJECTIVE: To investigate the effect of flavonoids from the seed residue and fruit residue of Hippophae rhamnoides L. (FSH and FFH) on glycometabolism in mice. METHOD: The healthy male mice were randomly divided into control group, three FSH treatment groups and three FFH treatment groups. FSH(50, 100, 150 mg/kg) and FFH (50, 100, 150 mg/kg) were given intragastorically(i.g.). At the same time, the mice of control group were given physiological saline. The levels of serum glucose, serum cholesterol were determined when it lasted 7 and 14 days. After 16 days glyconeogenesis test was made and liver glycogen was analyzed. RESULT: The levels of serum glucose, serum cholesterol and serum triglyceride were significantly reduced by high dose FSH and FFH. The glyconegenesis was also obviously inhibited by FSH and FFH. CONCLUSION: FSH and FFH can decrease the levels of blood glucose and lipid in normal mice, and the effect of FSH and FFH on glycometabolism may be related to the control of glyconeogenesis.


Assuntos
Glicemia/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Flavonoides/farmacologia , Hippophae/química , Animais , Glicemia/metabolismo , Colesterol/sangue , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/isolamento & purificação , Flavonoides/administração & dosagem , Flavonoides/isolamento & purificação , Frutas/química , Gluconeogênese/efeitos dos fármacos , Fígado/metabolismo , Masculino , Camundongos , Plantas Medicinais/química , Sementes/química , Triglicerídeos/sangue
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