Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 22
Filtrar
1.
J Mycol Med ; 31(2): 101048, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33127320

RESUMO

INTRODUCTION: In Mauritania, only an investigation carried out 26 years ago allows to report epidemiological findings about tinea capitis in Nouakchott. The objective of this study was to reactualize the tinea capitis epidemiological, clinical and mycological profile in school children and to compare this profile in urban and rural area. PATIENTS AND METHODS: All subscribed students in targeted schools and present during the investigation were examined clinically before samples collection. Mycological diagnosis was made by direct examination and culture in Sabouraud Dextrose Agar with chloramphenicol and supplemented with cycloheximide. The results were considered positive when culture with the presence of dermatophytes was obtained. RESULTS: Prevalence of tinea capitis among school children was 10.50% and it was more important in rural area (12.21%) than in urban area (9.52%). The age group ranged between 6 and 8 years was the most affected (13.58%). According to the gender, male (11.46%) were more affected than female (9.62%). Trichophytic tinea were predominant and Trichophyton soudanense was more isolated followed by Trichophyton rubrum, Trichophyton schoenleinii and Microsporum audouinii.


Assuntos
População Rural/estatística & dados numéricos , Tinha do Couro Cabeludo/epidemiologia , População Urbana/estatística & dados numéricos , Adolescente , Criança , Feminino , Humanos , Masculino , Mauritânia/epidemiologia , Microsporum/classificação , Microsporum/genética , Prevalência , Estudantes , Tinha do Couro Cabeludo/microbiologia , Trichophyton/classificação , Trichophyton/genética
2.
Ann Pharm Fr ; 79(4): 440-445, 2021 Jul.
Artigo em Francês | MEDLINE | ID: mdl-33232707

RESUMO

In the context of increasing incidence of fungal infections worldwide, the availability of antifungal drugs was studied in Senegal, a country with limited resources where diagnosis of invasive fungal infections is not often established by lack of specialists and/or diagnostic tools. After a background on the country's situation with regard to fungal infections and the drug distribution network in Senegal, a compilation of the various available antifungals was presented before concluding with recommendations to prepare for a situation that could allow to treat appropriately invasive mycoses.


Assuntos
Antifúngicos , Micoses , Antifúngicos/uso terapêutico , Humanos , Micoses/tratamento farmacológico , Micoses/epidemiologia , Senegal/epidemiologia
3.
J Mycol Med ; 30(3): 100974, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32417179

RESUMO

To determine fungal species distribution of interdigital intertrigo among seafarers in Dakar, Senegal, a cross-sectional study was carried out from May to August 2017 among seafarers clinically diagnosed with interdigital intertrigo. A questionnaire was filled to each patient before sampling the affected folds and transporting to Aristide Le Dantec University Hospital where mycological analyses were realized. Species identification by MALDI-TOF MS was performed in Marseille, France. In total, 169 men (21-66 years) were included. Few of them (3%) had a high level of education and the duration of the mycosis exceed 10 years for 88% of patients. Direct microscopic examination (ME) was positive in 34.3%. Among samples with positive ME, 58.6% had positive culture. An overall incidence of 30.2% was found. Patients with confirmed cases aged between 28 and 66 years. Among them, those between 36-50 years were predominant (52.9%). Those with a high level of education were less representative (2%). For 52.1% of patients, the duration of the mycosis was superior to 10 years. Furthermore, 57% of cases were significantly associated with other types of tinea pedis and/or onychomycosis (P=0.03). Culture was positive in 23.7% isolating 43 strains successfully identified at the species level by MALDI-TOF MS for 31 isolates: 20 Candida and 11 dermatophytes. The rest was identified only at the genus level belonged to Fusarium. In definitive, MALDI-TOF MS could be a useful tool for routine and fast identification of dermatophytes, yeasts and NDFF in clinical mycology laboratories.


Assuntos
Dermatoses do Pé/microbiologia , Intertrigo/microbiologia , Militares , Técnicas de Tipagem Micológica/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Adulto , Idoso , Candida/isolamento & purificação , Estudos Transversais , Dermatoses do Pé/epidemiologia , Humanos , Intertrigo/epidemiologia , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Militares/estatística & dados numéricos , Onicomicose/epidemiologia , Onicomicose/microbiologia , Senegal/epidemiologia , Tinha dos Pés/epidemiologia , Tinha dos Pés/microbiologia , Viagem , Trichophyton/classificação , Trichophyton/isolamento & purificação , Adulto Jovem
4.
Bull Soc Pathol Exot ; 112(3): 129-132, 2019.
Artigo em Francês | MEDLINE | ID: mdl-31825187

RESUMO

Mycetoma is transmitted by thorns infected. The commonest site for mycetoma is the foot. The primary pulmonary are rare and usually secondary to other primary site. We report a case of pulmonary fungal mycetoma secondary to primary site in the knee. We do a review of the literature and we discuss the way of dissemination.


Le mycétome se transmet principalement par piqures d'épines d'arbustes infectés. Les localisations primitives au niveau du pied sont les plus fréquentes. Les localisations pulmonaires sont exceptionnelles et secondaires à des localisations périphériques primitives. Nous rapportons un cas de localisation pulmonaire d'un mycétome fongique secondaire à une localisation au niveau du genou, puis nous faisons une revue de la littérature et nous discutons de la voie de dissémination.


Assuntos
Infecções Fúngicas Invasivas/diagnóstico , Traumatismos do Joelho/microbiologia , Pneumopatias Fúngicas/diagnóstico , Micetoma/etiologia , Ferimentos Penetrantes/complicações , Humanos , Infecções Fúngicas Invasivas/etiologia , Infecções Fúngicas Invasivas/microbiologia , Infecções Fúngicas Invasivas/patologia , Traumatismos do Joelho/complicações , Traumatismos do Joelho/diagnóstico , Pneumopatias Fúngicas/etiologia , Pneumopatias Fúngicas/microbiologia , Pneumopatias Fúngicas/patologia , Micetoma/diagnóstico , Senegal , Ferimentos Penetrantes/microbiologia
5.
J Mycol Med ; 29(2): 185-188, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30956063

RESUMO

Dermatophytic mycetoma of the scalp is a rarity, especially when not associated with tinea capitis. Here, we report a case of dermatophytic mycetoma of the scalp, that was not associated with tinea capitis, caused by an atypical Microsporum audouinii strain. To our knowledge, this is the second reported case, thirty-eight years after the first that was published in 1980.


Assuntos
Dermatomicoses/diagnóstico , Microsporum/isolamento & purificação , Micetoma/diagnóstico , Micetoma/microbiologia , Couro Cabeludo/microbiologia , Adulto , Antifúngicos/uso terapêutico , Biópsia , DNA Intergênico/genética , Dermatomicoses/tratamento farmacológico , Feminino , Humanos , Microsporum/efeitos dos fármacos , Microsporum/genética , Micetoma/tratamento farmacológico , Couro Cabeludo/patologia , Senegal , Análise de Sequência de DNA , Pele/patologia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
6.
J Mycol Med ; 28(4): 590-593, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30340859

RESUMO

Pityriasis versicolor (PV) is a superficial mycosis caused by yeast of the genus Malassezia. The most common isolated Malassezia species in PV lesions differ among M. furfur, M. globosa and M. sympodialis. We purpose to determine the distribution of Malassezia species in PV patients at the seafarers' medical service in Dakar, Senegal and to examine whether any association between identified Malassezia species and patients' profile. From May 2017 to August 2017, first a questionnaire was filled to get informative data before collection of skin scrapings taken from most scaly site using sterile scalpel blade and application of scotch® for direct examination (DE). At the laboratory, DE, culture and identification by MALDI-TOF MS were done. One hundred patients with PV - all men - were included with a mean age of 34 years. Among seafarers, 81% were sailors. Clinical prevalence of PV was highest in aged adults patients with ages of 31 to 60 years (56%). Seafarers with high level of education were less representative with only 2%. The mean duration of the PV was 26.83 months. 20% of subjects suffered lesions in more than one location. The chest was the most affected anatomical site. Furthermore, possible predisposing factors associated with PV were also detected. DE was positive in 95% but culture growth only in 46%. MALDI-TOF MS analysis of the positive cultures could be performed in 84.8% (39/46). Only M. furfur was identified in 100% (39/39). In definitive, M. furfur is the only causative agent of PV in Dakar.


Assuntos
Malassezia/classificação , Malassezia/isolamento & purificação , Tinha Versicolor/microbiologia , Adolescente , Adulto , Humanos , Malassezia/genética , Masculino , Pessoa de Meia-Idade , Militares/estatística & dados numéricos , Prevalência , Fatores de Risco , Senegal/epidemiologia , Pele/microbiologia , Pele/patologia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Tinha Versicolor/epidemiologia , Tinha Versicolor/patologia , Adulto Jovem
7.
J Mycol Med ; 28(2): 345-348, 2018 Jun.
Artigo em Francês | MEDLINE | ID: mdl-29548876

RESUMO

AIM: To determine the prevalence and the associated risk factors of vulvovaginal candidiasis in pregnant women in Mauritania. PATIENTS AND METHOD: The study was carried out at the laboratory of the Mère et Enfant Center in Nouakchott, from November 1, 2016 to February 5, 2017. It concerned all pregnant women in the third trimester that came to the natal consultation at the gynecology service. These women must have given their informed consent in written form. After completing the survey to collect epidemiological and clinical data, a sample was collected to perform fresh direct examination and culture on Sabouraud-Chloramphenicol media. RESULTS: Two hundred pregnant women were included in the study. Culture on Sabouraud-Chloramphenicol media was positive in 52 patients corresponding to a prevalence of 26%. The germ tube test was positive for 61.5% of isolated Candida. Clinical signs are dominated by leucorrhea (56%), pelvic pain (25%) and vulvar pruritus (11%). Among women with positive culture, 55.76% had received local antifungal treatment in the form of an ovula during pregnancy. CONCLUSION: This study shows that vulvovaginal candidiasis is frequent in pregnant women attending gynecology at the Mère et Enfant Hospital Center in Nouakchott.


Assuntos
Candidíase Vulvovaginal/epidemiologia , Complicações Infecciosas na Gravidez/epidemiologia , Feminino , Humanos , Mauritânia/epidemiologia , Gravidez , Prevalência , Fatores de Risco
8.
Med Sante Trop ; 28(4): 390-394, 2018 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-30799825

RESUMO

INTRODUCTION: Candida infections are most frequently encountered on the skin and nails. Their proportions vary according to geoclimatic and sociocultural conditions. The objective of this study was to determine the prevalence of skin and/or nail candidiasis diagnosed in the parasitology and mycology laboratory of Le Dantec University Hospital in Dakar, Senegal. MATERIALS AND METHODS: This study included 994 patients with suspected skin and/or nail mycosis tested at the parasitology and mycology laboratory during the 8-year study period of 2008-2015. Each patient had both a direct examination and a mycological culture. RESULTS: The patients' median age was 34 with an interquartile range of 25 years. Of these 994 patients, 613 had confirmed skins and/or nail fungal infections. In descending order, the fungi isolated and identified from these mycoses were Candida (n= 387, 63.1%), dermatophytes (28.4%), and mold (8.5%). Thus Candida accounted for nearly two thirds of the skin and/or nail mycoses and had a prevalence rate among the patients with suspected skin or nail candidiasis of 38.9%. These skin/nail sites was more common among women (72%) than men (28%), and slightly more than half of them (50,1%) were adults aged 31 to 60 years. The duration of candidiasis exceeded a full year for 76.3%. Onychomycoses were found more frequently (65.24%) than epidermomycoses, and both sites were infected in 5.17%. Candida albicans was the species isolated most frequently, accounting for 80% of Candida specimens. CONCLUSION: Our results reinforce the importance of mycological confirmation of the diagnosis. The mycology laboratory has a crucial role in making the differential diagnosis with other dermatoses of similar clinical appearance and thus in guiding the choice of treatment.


Assuntos
Candidíase/epidemiologia , Dermatomicoses/epidemiologia , Dermatoses da Mão/epidemiologia , Onicomicose/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Candidíase/diagnóstico , Criança , Pré-Escolar , Dermatomicoses/diagnóstico , Feminino , Dermatoses da Mão/diagnóstico , Hospitais Universitários , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Onicomicose/diagnóstico , Estudos Retrospectivos , Senegal/epidemiologia , Adulto Jovem
9.
J Mycol Med ; 28(1): 227-231, 2018 Mar.
Artigo em Francês | MEDLINE | ID: mdl-29132795

RESUMO

INTRODUCTION: Fungal interdigital tinea pedis (ITP) is a common pathology mainly due to dermatophytes and yeasts. Fusarium sp. is rarely incriminated in the genesis of intertrigo. In Dakar, a recent study conducted in 2016 on fungal ITP showed that Fusarium were more involved in the etiology of ITP than dermatophytes, coming just after yeasts dominated by Candida. Following this, we wanted to draw attention to the increasing incidence of ITP resulting from Fusarium spp., in Dakar, Senegal, and to analyze the epidemiological and mycological particularities of these ITP due to Fusarium spp. PATIENTS AND METHODS: A retrospective study including all patients received at the laboratory for suspicion of ITP between January 1st, 2014 and June 30th, 2017 was conducted. Diagnosis was based on mycological examination, including direct examination and culture. Mycological analysis was considered positive when direct examination and culture were positive after at least one repeat. RESULTS: Twenty-nine cases of Fusarium ITP accounting for 44.6% of all ITP in the study period were diagnosed in 15 men and 14 women. The mean age of the patients was 48.4 years. Fusarium ITP were diagnosed in immunocompetent patients except in two diabetics. The mean duration of the lesions was 6.83 years. The most frequent species isolated belonged to the Fusarium solani complex with 19 cases. CONCLUSION: Fusarium ITP in a healthy subject requires regular monitoring because any subsequent decrease in immune defenses could lead to fatal hematogenous spread.

10.
Bull Soc Pathol Exot ; 110(5): 286-290, 2017 Dec.
Artigo em Francês | MEDLINE | ID: mdl-29127650

RESUMO

Recently in Senegal, a case of Plasmodium ovale malaria had led to a diagnostic difficulty due to the ignorance of this parasite and the neglect of it. The objective of this study was to actively investigate cases of P. ovale malaria that would be misdiagnosed in the health centre structures of Senegal. The study was conducted in three areas that reflect different epidemiological strata of malaria. Microscopy was performed by microscopy experts on suspected malaria patients. The results were validated by Rougemont real-time PCR. Positive P. ovale cases were genotyped by nested PCR targeting the potra gene. A total of 406 samples were taken. Microscopy of Giemsa stained thick and thin smears recorded 228 cases of Plasmodium falciparum (97%), 3 cases of Plasmodium malariae (1.3%), and 4 cases of P. ovale (1.7%). The cases of P. ovale observed at microscopy were confirmed by real-time PCR. Genotyping of P. ovale revealed 3 cases of P. ovale wallikeri and 1 case of P. ovale curtisi. The prevalence of P. ovale malaria remains low in Senegal. However, malaria microscopists should be trained to recognize non-falciparum species in order to avoid the diagnostic delays and unnecessary investigations. National malaria control program should consider those species for the better management of malaria control in the country. Simplified molecular methods like, loop-mediated isothermal amplification (LAMP) may be useful to better characterize the epidemiology of non-falciparum malaria.


Assuntos
Malária/diagnóstico , Malária/epidemiologia , Malária/parasitologia , Plasmodium ovale , Adolescente , Estudos de Coortes , DNA de Protozoário/análise , Humanos , Malária/genética , Masculino , Tipagem Molecular/métodos , Plasmodium ovale/classificação , Plasmodium ovale/genética , Plasmodium ovale/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Senegal/epidemiologia
11.
Med Sante Trop ; 27(4): 431-434, 2017 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-29313513

RESUMO

To identify the protozoa responsible for intestinal parasitosis diagnosed at Le Dantec University Hospital in Dakar, Senegal. The retrospective study included 2578 patients for whom stool samples were analyzed from 2011 to 2015. The sex ratio was 1.1. Patients' mean age was 28.5 years, with a range of 11 days to 91 years. Direct examination and the Ritchie technique were performed to search for parasites. In total, 317 patients had intestinal protozoa, for a prevalence of 12.3 %. Infections involved one (91%) or two parasites (9%). The most common species found alone were Entamoeba coli (51.5%), E. histolytica/E. dispar (17 %), and Giardia intestinalis (10.5%). The most common associations were E. coli with G. intestinalis (3.6 %) and with E. histolytica (2.4%). These infections affected significantly more outpatients than inpatients (65 %, p = 0.0049) and slightly more women (51.1%, p = 0.22) than men. Adults accounted for 67% of the confirmed intestinal protozoa: 39% aged 31-60 years, 28% aged 15-30, and only 7% older than 60 (p = 0.5). Most of the protozoa isolated were non-pathogenic. However, health education should be promoted in addition to the mass treatment program already begun to limit and even halt the spread of these neglected diseases.


Assuntos
Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Hospitais Universitários , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Senegal/epidemiologia , Distribuição por Sexo , Adulto Jovem
12.
J Mycol Med ; 26(4): 398-402, 2016 Dec.
Artigo em Francês | MEDLINE | ID: mdl-27592053

RESUMO

A Senegalese lady of 17 years old presented right hand tinea unguium on all fingernails except the thumb lasting for 10 years. Mycological analysis showed with the direct examination 20% KOH mount numerous septate hyphae. Culture revealed Microsporum langeronii at a first time. The mycological analysis resumption with sampling scalp revealed a tinea capitis with M. langeronii then culture of nail pieces confirmed in a second time M. langeronii also associated with Trichophyton soudanense in the tinea unguium.


Assuntos
Microsporum/isolamento & purificação , Onicomicose/microbiologia , Tinha do Couro Cabeludo/diagnóstico , Trichophyton/isolamento & purificação , Adolescente , Diagnóstico Diferencial , Feminino , Humanos , Onicomicose/diagnóstico , Senegal , Tinha do Couro Cabeludo/microbiologia
13.
J Mycol Med ; 26(4): 368-376, 2016 Dec.
Artigo em Francês | MEDLINE | ID: mdl-27592054

RESUMO

INTRODUCTION: Superficial fungal infections, particularly of the skin, scalp and nails are very common and have been reported worldwide. The most common causative agents of these mycoses are dermatophytes, yeasts and molds. However, these agents vary with time and depend on many factors including the geography. The objective of this study was to identify the causative agents of superficial mycoses diagnosed at the Le Dantec University Hospital in Dakar (Senegal). MATERIAL AND METHODS: The study concerned 1851 outpatients received in the parasitological and mycological laboratory of Le Dantec hospital during the period from January 2011 to December 2015. Every patient benefited from direct examination and mycological culture. RESULTS: Among the 1851 patients, 633 were confirmed with superficial mycoses and the prevalence was 34.2 %. The age of patients ranged from two months to 81 years with a mean age of 31 years. Superficial mycoses were found more in women (70.3 %) than men (29.7 %) and a little more than thirty-nine percent (39.3 %) were adults. The causative agents identified were: dermatophytes (58 %), yeast (36.7 %) and non-dermatophytic filamentous fungi (NDFF, 5.3 %). The most isolated species were: Candida albicans (26.9 %), Trichophyton soudanense (24.9 %) and T. rubrum (13.7 %). These fungi were responsible for different clinical aspects, isolated and other associated. Among the isolated clinical aspects, those affecting hair (tinea capitis) were by far the largest with 44.8 %, followed by tinea unguium (34.5 %). Associations were especially type of tinea capitis and tinea manuum (2.4 %) and toenails onychomycosis associated with interdigital tinea pedis (2.7 %). Tinea capitis agents were exclusively dermatophytes and the predominant agent was T. soudanense with 47,8 %. Tinea unguium agents were yeast (79 %), dermatophytes (18 %) or NDFF (3 %) and the most found species was C. albicans (63.9 %). Dermatophytes, yeasts and molds were respectively found more in children and young adults, older adults and young adults and older adults and the elderly. CONCLUSION: In definitive, these epidemiological data should enable better diagnostic and therapeutic management of superficial mycoses.


Assuntos
Arthrodermataceae/classificação , Dermatomicoses/epidemiologia , Dermatomicoses/microbiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Arthrodermataceae/isolamento & purificação , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Senegal/epidemiologia , Adulto Jovem
14.
J Mycol Med ; 26(4): 312-316, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27184614

RESUMO

Fungal interdigital tinea pedis (FITP) is the most frequent dermatomycosis in industrial countries. In African tropics, it's a rare motive of consultation and is discovered while complicated. The aims of this article were: to determine the frequency of interdigital tinea pedis among overall mycological analysis in our laboratory; to study epidemiological, clinical and mycological aspects of FITP in outpatients attending the Le Dantec mycology laboratory in Dakar. A total of 62 males (60%) and 42 females (40%), mean age: 43.15 years (range: 11-81 years), were received from January 2011 to December 2015 for suspicion of FITP. Skin specimens were taken from all patients for microscopy and fungal culture. The frequency of ITP represents 5.6% (104/1851) among our overall mycological analysis. FITP was confirmed in 68 patients (SPI=65.38%), mainly located between the 4th and 5th toes and 71 fungal species were isolated (CPI=68.27%). Among patients with confirmed FITP, there were 38 males (56%) and 30 females (44%). The prevalence was highest in patients between 44 and 54 years (26%). Candida albicans, Fusarium solani and Trichophyton interdigitale were shown to be the most common pathogens respectively for yeasts (39%), non-dermatophytic filamentous fungi (NDFF; 21%) and dermatophytes (11%). So FITP isn't a common reason for consultation in Dakar but its simple parasitic index (SPI) is still very high and dermatophytes formerly the main causative agents are being relegated to third place behind yeasts and NDFF.


Assuntos
Tinha dos Pés/epidemiologia , Dedos do Pé/microbiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Senegal/epidemiologia , Tinha dos Pés/microbiologia , Adulto Jovem
15.
J Mycol Med ; 26(4): 317-322, 2016 Dec.
Artigo em Francês | MEDLINE | ID: mdl-27198757

RESUMO

INTRODUCTION: Endodontic flora is dominated in the apical part of the channels by strict anaerobic and some facultative anaerobic bacteria but also by Candida yeasts, especially Candida albicans species that are involved in the maintenance and persistence of endodontic infections. Their elimination of the canal system in practice by chemo-mechanical methods of disinfection is not always guaranteed. Thus, this in vitro study was performed to determine the sensitivity of C. albicans with sodium hypochlorite (NaOCl) dosed at 2.5 %, the chlorhexidine digluconate 0.5 % and calcium hydroxide used in inter-session medication. METHODS: The diffusion method was used initially to test the sensitivity of C. albicans strains with the above products. Then a dilution technique has allowed us to determine the minimum inhibitory concentration of these active products on C. albicans. RESULTS: Strains from infected pulp teeth of patients showed a sensitivity of C. albicans to sodium hypochlorite to a minimum inhibitory concentration less than 70µg/mL and 30µg/mL for chlorhexidine. CONCLUSION: This study demonstrated a sensitivity of C. albicans to sodium hypochlorite and chlorhexidine.


Assuntos
Hidróxido de Cálcio/farmacologia , Candida albicans/efeitos dos fármacos , Clorexidina/análogos & derivados , Desinfetantes/farmacologia , Hidróxido de Sódio/farmacologia , Anti-Infecciosos Locais/farmacologia , Candida albicans/crescimento & desenvolvimento , Candida albicans/isolamento & purificação , Clorexidina/farmacologia , Combinação de Medicamentos , Humanos , Testes de Sensibilidade Microbiana , Mucosa Bucal/microbiologia
16.
J Mycol Med ; 26(2): 111-115, 2016 Jun.
Artigo em Francês | MEDLINE | ID: mdl-26924766

RESUMO

INTRODUCTION: Endodontic infections are characterized by their microbial polymorphism with the presence of pathogenic agents such as bacteria. The aim of this study was to investigate the presence of yeast in the root canals of teeth with pulp necrotic with or without apical periodontitis and to study the sensibility of these at disinfection at sodium hypochlorite to 2.5 %. METHODS: Root canal samples, taken with paper points before and after disinfection with sodium hypochlorite dosed at 2.5 % on 50 single rooted teeth in 38 patients, were seeded in petri dishes containing Sabouraud Chloramphenicol (SC) (Conda Laboratories, Madrid). These stains were incubated in an incubator at 37°C for 48hours. The presence of yeast was confirmed by white colonies on the entire circumference of the tip paper. The identification of species was done by macroscopic and microscopic examinations associated with Blastese test. RESULTS: The yeast colonies were isolated from 7 teeth (14 %) out of 50. All positive samples were from teeth with an open necrotic pulp (P<0.001). The only species found was Candida albicans. Immediate disinfection with sodium hypochlorite at 2.5 % did not show a complete elimination of yeasts. CONCLUSION: The results of this study show that the canal of necrotic teeth with or without apical periodontitis may shelter yeasts even after immediate disinfection with sodium hypochlorite 2.5 %.


Assuntos
Necrose da Polpa Dentária/microbiologia , Periodontite Periapical/microbiologia , Hipoclorito de Sódio/farmacologia , Leveduras/efeitos dos fármacos , Leveduras/isolamento & purificação , Adolescente , Adulto , Idoso , Candida albicans/efeitos dos fármacos , Candida albicans/isolamento & purificação , Criança , Desinfecção/métodos , Feminino , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Senegal , Irrigação Terapêutica , Adulto Jovem
17.
J Mycol Med ; 25(4): e134-7, 2015 Dec.
Artigo em Francês | MEDLINE | ID: mdl-26597147

RESUMO

In developing countries where systemic antifungal are often unavailable, treatment of filamentous fungi infection as Fusarium is sometimes very difficult to treat. We report the case of a keratomycosis due to Fusarium oxysporum treated by povidone iodine eye drops and oral fluconazole. The diagnosis of abscess in the cornea was retained after ophthalmological examination for a 28-year-old man with no previous ophthalmological disease, addressed to the Ophthalmological clinic at the University Hospital Le Dantec in Dakar for a left painful red eye with decreased visual acuity lasting for 15 days. The patient did not receive any foreign body into the eye. Samples by corneal scraping were made for microbiological analysis and the patient was hospitalized and treated with a reinforced eye drops based treatment (ceftriaxone+gentamicin). The mycological diagnosis revealed the presence of a mold: F. oxysporum, which motivated the replacement of the initial treatment by eye drops containing iodized povidone solution at 1% because of the amphotericin B unavailability. Due to the threat of visual loss, oral fluconazole was added to the local treatment with eye drops povidone iodine. The outcome was favorable with a healing abscess and visual acuity amounted to 1/200th. Furthermore, we noted sequels such as pannus and pillowcase. The vulgarization of efficient topical antifungal in developing countries would be necessary to optimize fungal infection treatment.


Assuntos
Úlcera da Córnea/tratamento farmacológico , Infecções Oculares Fúngicas/tratamento farmacológico , Fluconazol/administração & dosagem , Fusarium/isolamento & purificação , Povidona-Iodo/administração & dosagem , Administração Oral , Adulto , Úlcera da Córnea/microbiologia , Quimioterapia Combinada , Infecções Oculares Fúngicas/microbiologia , Fusarium/fisiologia , Humanos , Masculino , Soluções Oftálmicas
18.
J Mycol Med ; 25(3): 181-90, 2015 Sep.
Artigo em Francês | MEDLINE | ID: mdl-26138533

RESUMO

In recent years, the incidence of superficial fungal infections involving nondermatophytic and noncandidal fungi increased considerably. The objective of this work was to analyze the epidemiological, clinical and mycological fungal infections due to nondermatophytic and noncandidal fungi diagnosed in the laboratory of parasitology-mycology of Le Dantec hospital in Dakar. With a retrospective study of the various cases of nondermatophytic and noncandidal fungi isolated in the laboratory of parasitology-mycology during the period of November 2013 to December 2014, we collected 22 cases of infections in 11 men and 11 women; age ranging from 17 to 75 years with a mean of 45.3 years (sex ratio=1): eight cases of intertrigo, seven cases of onychomycosis, four cases of palmoplantar keratoderma (KPP), a case of onychomycosis associated with interdigital intertrigo, a case of infectious myositis and one case of African histoplasmosis. We have isolated and identified a total of 22 nondermatophytic and noncandidal fungi: ten Fusarium, five Trichosporon, two Chrysosporium, two Geotrichum, one Rhodotorula, one Neoscytalidium dimidiatum and one Histoplasma capsulatum var. duboisii. So we are seeing the emergence of nondermatophytic and noncandidal increasingly isolated from superficial and local lesions. These fungi, generally contaminants or commensal, cause a problem regarding their direct involvement in pathological processes in which they are isolated. So we should respect the recommendations proposed for their involvement in pathological processes and, by a collaboration between clinician and biologist, demonstrate their real involvement through effective, targeted treatment.


Assuntos
Dermatomicoses/epidemiologia , Dermatomicoses/microbiologia , Adolescente , Adulto , Idoso , Feminino , Dermatoses do Pé/epidemiologia , Dermatoses do Pé/microbiologia , Fungos/isolamento & purificação , Histoplasmose/epidemiologia , Histoplasmose/microbiologia , Hospitais Universitários/estatística & dados numéricos , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Onicomicose/epidemiologia , Onicomicose/microbiologia , Estudos Retrospectivos , Senegal/epidemiologia , Adulto Jovem
19.
J Mycol Med ; 25(2): 169-76, 2015 Jun.
Artigo em Francês | MEDLINE | ID: mdl-25921492

RESUMO

INTRODUCTION: Tinea capitis is considered as a public health problem in Senegal. The aim of this study was to investigate trends in the incidence, the mycological and epidemiological aspects of tinea capitis diagnosed at Le Dantec Hospital in Dakar. METHOD: Our work is a retrospective study concerning all scalp samples taken by the parasitology laboratory, over a 6-year period (2008-2013). RESULTS: A total of 1640 specimens were examined. Of these, 566 were positive with direct examination and after culture. We noted the reduction of patients and of the incidence of tineas during 6 years with variations of 147 (46.82%) to 37 (22.02%). The average incidence of the tineas during six years was 34.51%. Patients' age varied between 1 to 83 years with a mean of 27.33 years. Prevalence varied between age groups, with 10.61 % in adults between 20 to 29 years, 7.19% in children between 0 to 9 years, 6.04% between 10 to 19 years, and 5.91% in adults between 30 to 39 years. Women were more infected 469 (82.9%) than men 97 (17.1%). The main dermatophytes isolated were: T. soudanense in 318 cases (56.18%), T. rubrum in 104 cases (18.37%), M. langeronii in 72 cases (12.72%), M. canis in 36 cases (6.36%), and T. mentagrophytes in 26 cases (4.60%). CONCLUSION: Our study showed a decrease in the annual incidence of tinea capitis over the study period with an evident increase in trichophytic tinea. This study showed that tinea is endemic in Senegal mainly among women between 20 and 29 years.


Assuntos
Tinha do Couro Cabeludo/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Senegal/epidemiologia , Tinha do Couro Cabeludo/microbiologia , Adulto Jovem
20.
J Mycol Med ; 25(2): 155-8, 2015 Jun.
Artigo em Francês | MEDLINE | ID: mdl-25835156

RESUMO

A patient presented with intertrigo at the second, third and fourth interdigitals spaces lasting for four years in which Fusarium solani was highlighted. The search for contributing factors revealed a concept of foot washing with water at least five times a day for ablutions, associated with wearing closed shoes all day and the absence of immunosuppression and diabetes. The diagnosis of Fusarium was made on the basis of direct examination and culture. Combined treatment with griseofulvin oral and topical ciclopirox was introduced and allowed healing after 45 days at which an antifungal powder was prescribed for relay. This case adds to the rare cases of intertrigo Fusarium sp. and confirms the frequent practice of ablutions as favoring factor.


Assuntos
Fusarium/isolamento & purificação , Tinha dos Pés/microbiologia , Adulto , Antifúngicos/administração & dosagem , Ciclopirox , Griseofulvina/administração & dosagem , Humanos , Masculino , Piridonas/administração & dosagem , Senegal , Tinha dos Pés/tratamento farmacológico
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA