RESUMO
In recent years, researchers have turned to transient gene expression (TGE) as an alternative to CHO stable cell line generation for early-stage antibody development. Despite advances in transfection methods and culture optimization, the majority of CHO-based TGE systems produce insufficient antibody titers for extensive use within biotherapeutic development pipelines. Flow electroporation using the MaxCyte STX Scalable Transfection System is a highly efficient, scalable means of CHO-based TGE for gram-level production of antibodies without the need for specialized expression vectors or genetically engineered CHO cell lines. CHO cell flow electroporation is easily scaled from milligram to multigram quantities without protocol reoptimization while maintaining transfection performance and antibody productivity. In this article, data are presented that demonstrate the reproducibility, scalability, and antibody production capabilities of CHO-based TGE using the MaxCyte STX. Data show optimization of posttransfection parameters such as cell density, media composition, and feed strategy that result in secreted antibody titers >1 g/L and production of multiple grams of antibody within 2 weeks of a single CHO-S cell transfection. In addition, data are presented to demonstrate the application of scalable electroporation for the rapid generation of high-yield stable CHO cell lines to bridge the gap between early- and late-stage antibody development activities.
Assuntos
Eletroporação , Expressão Gênica , Animais , Células CHO , Cricetinae , CricetulusAssuntos
American Heart Association/organização & administração , Cardiologia/organização & administração , Terapia Cognitivo-Comportamental/métodos , Doença da Artéria Coronariana/prevenção & controle , Obesidade/terapia , Sobrepeso/terapia , Adulto , Pressão Sanguínea , Índice de Massa Corporal , Humanos , Estilo de Vida , Prevalência , Prognóstico , Sociedades Médicas , Estados Unidos/epidemiologiaAssuntos
Pesquisa Biomédica/métodos , National Heart, Lung, and Blood Institute (U.S.)/tendências , Obesidade , Avaliação de Programas e Projetos de Saúde , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/etiologia , Doenças Cardiovasculares/prevenção & controle , Humanos , Morbidade/tendências , Obesidade/complicações , Obesidade/epidemiologia , Obesidade/terapia , Estados Unidos/epidemiologiaRESUMO
A cluster of risk factors for cardiovascular disease and type 2 diabetes mellitus, which occur together more often than by chance alone, have become known as the metabolic syndrome. The risk factors include raised blood pressure, dyslipidemia (raised triglycerides and lowered high-density lipoprotein cholesterol), raised fasting glucose, and central obesity. Various diagnostic criteria have been proposed by different organizations over the past decade. Most recently, these have come from the International Diabetes Federation and the American Heart Association/National Heart, Lung, and Blood Institute. The main difference concerns the measure for central obesity, with this being an obligatory component in the International Diabetes Federation definition, lower than in the American Heart Association/National Heart, Lung, and Blood Institute criteria, and ethnic specific. The present article represents the outcome of a meeting between several major organizations in an attempt to unify criteria. It was agreed that there should not be an obligatory component, but that waist measurement would continue to be a useful preliminary screening tool. Three abnormal findings out of 5 would qualify a person for the metabolic syndrome. A single set of cut points would be used for all components except waist circumference, for which further work is required. In the interim, national or regional cut points for waist circumference can be used.
Assuntos
Gordura Abdominal , Síndrome Metabólica/diagnóstico , Obesidade/diagnóstico , Circunferência da Cintura , Glicemia/metabolismo , Doenças Cardiovasculares/etiologia , HDL-Colesterol/sangue , Diabetes Mellitus/etiologia , Humanos , Hipertensão/complicações , Resistência à Insulina , Síndrome Metabólica/sangue , Síndrome Metabólica/etiologia , Obesidade/sangue , Obesidade/complicações , Fatores de Risco , Terminologia como Assunto , Triglicerídeos/sangueRESUMO
The national education programs and campaigns described here are examples of the many unique kinds of federal efforts under way to promote the pillars of healthy eating and increased physical activity included in the "Healthier US Initiative." They are similar in that: 1) they are based on the best available science that a health problem exists, and 2) that healthy eating and physical active behaviors will improve health status. They are unique in their implementation, for example, in private/public partnerships, coordinating committees of professional associations, and congressionally mandated interventions. Most importantly, they provide the impetus to get a particular health issue on the public agenda.
Assuntos
Dieta/normas , Exercício Físico/fisiologia , Educação em Saúde , Promoção da Saúde , Ingestão de Alimentos , HumanosAssuntos
Síndrome Metabólica/terapia , Diabetes Mellitus Tipo 2/epidemiologia , Diabetes Mellitus Tipo 2/prevenção & controle , Humanos , Lipoproteínas/sangue , Síndrome Metabólica/complicações , Síndrome Metabólica/diagnóstico , Síndrome Metabólica/epidemiologia , National Institutes of Health (U.S.) , Fatores de Risco , Sociedades Médicas , Estados Unidos/epidemiologiaAssuntos
Estilo de Vida , Síndrome Metabólica/diagnóstico , Síndrome Metabólica/terapia , American Heart Association , Aterosclerose/complicações , Aterosclerose/terapia , Coagulação Sanguínea/efeitos dos fármacos , Glicemia/efeitos dos fármacos , Glicemia/metabolismo , Pressão Sanguínea/efeitos dos fármacos , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/terapia , Diagnóstico Diferencial , Dislipidemias/complicações , Dislipidemias/terapia , Humanos , Hipertensão/complicações , Hipertensão/terapia , Síndrome Metabólica/tratamento farmacológico , Síndrome Metabólica/etiologia , Síndrome Metabólica/prevenção & controle , National Institutes of Health (U.S.) , Obesidade/complicações , Obesidade/terapia , Fatores de Risco , Estados UnidosAssuntos
Obesidade/epidemiologia , Obesidade/prevenção & controle , Adolescente , Adulto , Idoso , Índice de Massa Corporal , Criança , Pré-Escolar , Serviços de Saúde Comunitária , Etnicidade , Saúde da Família , Feminino , Saúde Global , Educação em Saúde , Política de Saúde , Promoção da Saúde , Cardiopatias/epidemiologia , Cardiopatias/etiologia , Cardiopatias/prevenção & controle , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Obesidade/complicações , Serviços de Saúde do Trabalhador , Guias de Prática Clínica como Assunto , Prevalência , Fatores de Risco , Serviços de Saúde Escolar , Acidente Vascular Cerebral/epidemiologia , Acidente Vascular Cerebral/etiologia , Acidente Vascular Cerebral/prevenção & controleRESUMO
OBJECTIVE: To provide insight into discussions at the Surgeon General's Listening Session, "Toward a National Action Plan on Overweight and Obesity," and to complement The Surgeon General's Call to Action to Prevent and Decrease Overweight and Obesity. RESEARCH METHODS AND PROCEDURES: On December 7 and 8, 2000, representatives from federal, state, academic, and private sectors attended the Surgeon General's Listening Session and were given an opportunity to recommend what to include in a national plan to address overweight and obesity. The public was invited to comment during a corresponding public comment period. The Surgeon General's Listening Session was also broadcast on the Internet, allowing others to view the deliberations live or access the archived files. Significant discussion points from the Listening Session have been reviewed by representatives of the federal agencies and are the basis of this complementary document. RESULTS: Examples of issues, strategies, and barriers to change are discussed within five thematic areas: schools, health care, family and community, worksite, and media. Suggested cooperative or collaborative actions for preventing and decreasing overweight and obesity are described. An annotated list of some programmatic partnerships is included. DISCUSSION: The Surgeon General's Listening Session provided an opportunity for representatives from family and community groups, schools, the media, the health-care environment, and worksites to become partners and to unite around the common goal of preventing and decreasing overweight and obesity. The combination of approaches from these perspectives offers a rich resource of opportunity to combat the public health epidemic of overweight and obesity.