Colloidal Nanoparticles of a ß-Cyclodextrin/L-Tryptophan Inclusion Complex for Use as Pickering Emulsion Stabilizers.

Inclusion complexes of ß-cyclodextrin (ß-CD) and tryptophan (Trp) were synthesized using an antisolvent approach, and fully characterized. Scanning electron microscope images proved the formation of the ß-CD/Trp NPs and the powder X-ray diffraction pattern indicated the formation of a crystalline channel-like structure for the ß-CD/Trp nanoparticles (NPs). The NPs of a ß-CD/Trp inclusion complex were used as a natural stabilizer at the oil/water interface of a Pickering emulsion. Pickering emulsions with an oil to water ratio of 1:1 (v:v) were obtained under high-speed homogenization and different mass ratios of the ß-CD to Trp (1:0, 1:0.1, 1:0.25, 1:0.5, 1:1), and at different pH levels (3, 5, 7, 9). At pH 9, when the ß-CD:Trp mass ratio was 1:0.1, the ß-CD/Trp NPs were hydrophilic, and the oil-in-water Pickering emulsions stabilized by those nanoparticles showed the highest storage stability: 180 days at room temperature. In contrast, when the emulsions were prepared at pH 5 with the weight ratios of either 1:0.1 or 1:1, ß-CD:Trp, the nanoparticles were hydrophobic and could be used to stabilize water-in-oil Pickering emulsions.

Dual mechanism of membrane covering on GHG and NH3 mitigation during industrial-scale experiment on dairy manure composting: Inhibiting formation and blocking emissions.

An industrial-scale experiment on dairy manure composting with the control group (Ctrl) and the membrane covering group (CM) was conducted to explore the effects of functional membrane covering on gas emissions, the conversion of carbon and nitrogen, and revealing the underlying mechanisms. Results indicated that CM achieved the synergistic effects on gas mitigation and improved compost product quality. CO2, CH4, N2O, and NH3 emissions were reduced by 81.8%, 87.0%, 82.6%, and 82.2%, respectively. The micro-aerobic condition formed in membrane covering compost pile together with the covering inhibiting effect dominated the mitigation effect. CM significantly downregulated the mcrA gene copies and the value of mcrA/pmoA (p < 0.01), which reduced CH4 emission. CM decreased the nirS and nirK gene copies and increased the nosZ gene copies to reduce N2O emission. Functional Annotation of Prokaryotic Taxa showed that membrane covering effectively amended part of carbon and nitrogen cycles, which stimulated the degradation of organic matter, accelerated compost maturity and reduced the gaseous emissions.

Using transglutaminase to cross-link complexes of lactoferrin and α-lactalbumin to increase thermal stability.

The poor thermal stability of lactoferrin (LF) hinders its bioavailability and use in commercial food products. To preserve LF from thermal denaturation, complexation with other biopolymers has been studied. Here we present the complex formation conditions, structural stability, and functional protection of LF by α-lactalbumin (α-LA). The formation of the LF-α-LA complexes was dependent on pH, mass ratio, and ionic strength. Changing the formation conditions and cross-linking by transglutaminase impacted the turbidity, particle size, and zeta-potential of the resulting complexes. Electrophoresis, Fourier-transform infrared spectroscopy, and circular dichroism measurements suggest that the secondary structure of LF in the LF-α-LA complex was maintained after complexation and subsequent thermal treatments. At pH 7, the LF-α-LA complex protected LF from thermal aggregation and denaturation, and the LF retained its functional and structural properties, including antibacterial capacity of LF after thermal treatments. The improved thermal stability and functional properties of LF in the LF-α-LA complex are of interest to the food industry.

Mitigation potential of methane emissions in China's livestock sector can reach one-third by 2030 at low cost.

The mitigation of methane (CH4) emissions from livestock production is crucial to China's carbon neutrality. Here we established a high-spatiotemporal-resolution dataset of the country's livestock CH4 emissions from 1990 to 2020 using four large-scale national livestock greenhouse gas inventory surveys. We estimate CH4 emissions to be 14.1 ± 2.0 Mt in 2020 and to increase by 13% until 2030 despite CH4 intensity per kg animal protein having decreased by 55% in the past 30 years. Approximately half of the emissions come from 13% of all Chinese counties. The technical CH4 mitigation potential is projected to be 36 ± 8% (4.4-6.9 Mt CH4) in 2030, and reducing food loss and waste could mitigate an additional 1.6 Mt of CH4. Overall, most CH4 mitigation could be achieved by increasing animal productivity and coverage of lagoon storage at carbon prices below US$100 tCO2e-1, being more cost-effective than livestock nitrous oxide mitigation in China.

Enhanced lactic acid production through enzymatic hydrolysis: Assessing impact of varied enzyme loadings on co-fermentation of swine manure and apple waste.

Anaerobic co-fermentation of swine manure (SM) and apple waste (AW) restricts by the slow hydrolysis of substrates with complex structures, which subsequently leads to low lactic acid (LA) production. Therefore, a novel strategy based on enzymatic pretreatment for improving LA production from anaerobic co-fermentation of SM and AW was proposed in this study. The results indicated that the maximal LA concentration increased from 35.89 ± 1.84 to 42.70 ± 2.18 g/L with the increase of enzyme loading from 0 to 300 U/g VSsubstrate. Mechanism exploration indicated that enzymatic pretreatment significantly promoted the release and hydrolysis of insoluble organic matter from fermentation substrate, thus providing an abundance of reaction intermediates that were directly available for LA production. Additionally, bacteria analysis revealed that the high concentration of LA was associated with the prevalence of Lactobacillus. This study offered an environmental-friendly strategy for promoting SM and AW hydrolysis and provided a viable approach for recovering valuable products.

Lactic acid production with two types of feedstocks from food waste: Effect of inoculum, temperature, micro-oxygen, and initial pH.

Lactic acid (LA) is an important chemical with broad market applications. To optimize LA production, food waste has been explored as feedstock. Due to the wide variety of food waste types, most current research studies have obtained different conclusions. This study focuses on carbohydrate-rich fruit and vegetable waste (FVW) and lipid-rich kitchen waste (KW), and the effect of inoculum, temperature, micro-oxygen, and initial pH were compared. FVW has a greater potential for LA production than KW. As an inoculum, lactic acid bacteria (LAB) significantly increased the maximum LA concentration (27.6 g/L) by 50.8 % compared with anaerobic sludge (AS). FVW exhibited optimal LA production at 37 °C with micro-oxygen. Adjustment of initial pH from 4 to 8 alleviated the inhibitory effect of accumulated LA, resulting in a 46.2 % increase in maximum LA production in FVW. The expression of functional genes associated with metabolism, genetic information processing, and environmental information processing was higher at 37 °C compared to 50 °C.

Enzyme enhanced lactic acid fermentation of swine manure and apple waste: Insights from organic matter transformation and functional bacteria.

Anaerobic co-fermentation is a favorable way to convert agricultural waste, such as swine manure (SM) and apple waste (AW), into lactic acid (LA) through microbial action. However, the limited hydrolysis of organic matter remains a main challenge in the anaerobic co-fermentation process. Therefore, this work aims to deeply understand the impact of cellulase (C) and protease (P) ratios on LA production during the anaerobic co-fermentation of SM with AW. Results showed that the combined use of cellulase and protease significantly improved the hydrolysis during the enzymatic pretreatment, thus enhancing the LA production in anaerobic acidification. The highest LA reached 41.02 ± 2.09 g/L within 12 days at the ratio of C/P = 1:3, which was approximately 1.26-fold of that in the control. After a C/P = 1:3 pretreatment, a significant SCOD release of 45.34 ± 2.87 g/L was achieved, which was 1.13 times the amount in the control. Moreover, improved LA production was also attributed to the release of large amounts of soluble carbohydrates and proteins with enzymatic pretreated SM and AW. The bacterial community analysis revealed that the hydrolytic bacteria Romboutsia and Clostridium_sensu_stricto_1 were enriched after enzyme pretreatment, and Lactobacillus was the dominant bacteria for LA production. This study provides an eco-friendly technology to enhance hydrolysis by enzymatic pretreatment and improve LA production during anaerobic fermentation.

The influence of using different types of modified vermiculite cover on ammonia mitigation from animal slurry storage: The role of sulfuric acid.

Animal slurry storage is an important ammonia (NH3) emission source. Sulfuric acid (H2SO4)-modified vermiculite coverage is a new promising technology for controlling NH3 emission from slurry storage. However, the underlying mechanisms in controlling the mitigation effect remain unclear. Here, a series of experiments to determine the effect of H2SO4 on the modified vermiculite properties, floating persistence, and NH3 mitigation effect was conducted. Results showed that abundant H2SO4 and sulfate remained on the outer surface and in the extended inner pores of the vermiculite with acidifying H+ concentrations higher than 5 M. An initial strong instantaneous acidification of surface slurry released rich carbon dioxide bubbles, strengthening cover floating performance. An acidification in the vermiculite cover layer and a good coverage inhibition interacted, being the two leading mechanisms for mitigating NH3 during initial 40-50 days of storage. The bacterial-amoA gene dominated the conversion of NH3 to nitrous oxide after 50 days of storage. Vermiculite with 5 M H+ modification reduced the NH3 emissions by 90 % within the first month of slurry storage and achieved a 64 % mitigation efficiency throughout the 84 days period. With the development of the aerial spraying equipment such as agricultural drones, acidifying vermiculite coverage hold promise as an effective method for reducing NH3 emission while absorbing nutrients from liquid slurry storage tank or lagoon. This design should now be tested under field conditions.

Effect of wheat crops on the persistence and attenuation of antibiotic resistance genes in soil after swine wastewater application.

Swine wastewater (SW) application introduces antibiotic resistance genes (ARGs) into farmland soils. However, ARG attenuation in SW-fertigated soils, especially those influenced by staple crops and soil type, remains unclear. This study investigated twelve soil ARGs and one mobile genetic element (MGE) in sandy loam, loam, and silt loam soils before and after SW application in wheat-planted and unplanted soils. The results revealed an immediate increase in the abundance of ARGs in soil by two orders of magnitude above background levels following SW application. After SW application, the soil total ARG abundance was attenuated, reaching background levels at 54 days; However, more individual ARGs were detected above the detection limit than pre-application. Among the 13 genes, acc(6')-lb, tetM, and tetO tended to persist in the soil during wheat harvest. ARG half-lives were up to four times longer in wheat-planted soils than in bare soils. Wheat planting decreased the persistence of acc(6')-lb, ermB, ermF, and intI2 but increased the persistence of others such as sul1 and sul2. Soil type had no significant impact on ARG and MGE fates. Our findings emphasize the need for strategic SW application and the consideration of crop cultivation effects to mitigate ARG accumulation in farmland soils.

Improving the thermal stability of phytase using core-shell hydrogel beads.

A core-shell hydrogel bead system was designed to maintain the catalytic activity of phytase and protect its enzymatic functionality from heat treatment. The designed structure consists of a chitosan-phytase complex core and an alginate-carrageenan hydrogel shell. The core-shell hydrogel was optimized to improve phytase encapsulation efficiency and increase the thermal stability of the encapsulated phytase. After heat treatment, encapsulated phytase retained âˆ¼ 70 % of its catalytic activity and the same secondary structure of free phytase. Fourier transform infrared spectroscopy indicated strong intermolecular interactions between chitosan and phytase in the core, but little interaction between the core and the alginate and κ-carrageenan shell, this supports the structural and functional stability of the phytase. Differential scanning calorimetry confirmed that the designed core-shell structure had a higher melting point. Encapsulating phytase in a core-shell hydrogel bead can enhance the thermal stability of phytase, which broadens the potential applications for phytase delivery.

Responses of lettuce (Lactuca sativa L.) growth and soil properties to conventional non-biodegradable and new biodegradable microplastics.

Residual plastic films in soils are posing a potential threat to agricultural ecosystem. However, little is known about the impacts of microplastics (MPs) derived from biodegradable and non-biodegradable plastic films on plant-soil systems. Here, we carried out a pot experiment using soil-cultivated lettuce treated by two types of MPs, degradable poly(butylene adipate-co-terephthalate) (PBAT-MPs) and non-biodegradable polyethylene (PE-MPs). MPs resulted in different degrees of reduction in shoot biomass, chlorophyll content, photosynthetic parameters, and leaf contents of nitrogen (N), phosphorus (P), and potassium (K), accelerated accumulation of hydrogen peroxide and superoxide, and increased malondialdehyde content in lettuce leaves. Moreover, MPs obviously decreased contents of total N, nitrate, ammonium, and available K in soils, and increased available P, thus altering soil nutrient availability. MPs also significantly decreased proportions of macroaggregates, and decreased soil electrical conductivity and microbial activity. PBAT-MPs had significantly greater impacts on oxidative damage, photosynthetic rate, soil aggregation, microbial activity, and soil ammonium than those of PE-MPs. Our results suggested that MPs caused oxidative damages, nutrient uptake inhibition, soil properties alteration, ultimately leading to growth reduction, and PBAT-MPs exhibited stronger impacts. Therefore, it is urgent to further study the ecological effects of MPs, especially biodegradable MPs, on soil-plant systems.

Variation of PM2.5 and PM10 in emissions and chemical compositions in different seasons from a manure-belt laying hen house.

Particulate matter (PM) emissions from animal houses and the corresponding hazard have raised increasing attention during recent years. In this study, a large-scale manure-belt laying hen house located in Beijing, China was selected as the experimental site for the study of the emission rates (ER) and chemical compositions of PM2.5 and PM10 in 3 seasons, namely, summer, autumn, and winter, to investigate their possible influences on ambient air quality and human health. The results showed that the mean ER from the hen house in summer, autumn, and winter were 9.0 ± 1.7, 2.4 ± 0.7, and 1.9 ± 0.7 mg hen-1 d-1 for PM2.5 (P < 0.05), and 30.7 ± 1.1, 12.8 ± 1.5, and 10.9 ± 0.9 mg hen-1 d-1 for PM10 (P < 0.05), respectively. Moreover, large amounts of secondary inorganic aerosols (SIA) were observed inside the house in summer, accounting for 11.4 and 9.6% of indoor PM2.5 and PM10 mass, respectively, compared with the value of <1.4% in autumn and winter. Among the 31 detected elements in indoor PM, arsenic concentration exceeded the threshold set in legislation. Zn had a notably high concentration of 3,403 to 4,432 ng m-3 in indoor PM10, which was 28 to 71 times higher than that in ambient PM10. The findings suggest that the poultry-raising house emit PM2.5 and PM10 containing SIA and toxic heavy-metal elements such as As and Zn to the ambient with much more emissions in summer than in autumn and winter. Considering the increasing development of poultry-raising farming in China, the potential hazard derived from the exhaust of PM2.5 and PM10 should be focused on, especially during summer.

Responses of CH4, N2O, and NH3 emissions to different slurry pH values of 5.5-10.0: Characteristics and mechanisms.

Animal slurry storage is a significant source of greenhouse gas (GHG) and ammonia (NH3) emissions. pH is a basic but key factor that could pose great influence on gas emissions, but the simultaneous evaluation of its influence on GHG and NH3 emissions and the understanding of its underlying mechanism are not enough. In this work, pH was adjusted between 5.5 and 10.0 by a step of 0.5 unit by adding lactic acid and sodium hydroxide (NaOH) properly and frequently to the stored slurry during a 43-day storage period. The cumulative NH3 emissions were linearly correlated with the slurry pH, with R2 being 0.982. Maintaining the slurry pH at 5.5-6.0 could reduce NH3 emissions by 69.4%-85.1% compared with the non-treated group (CK). The pH ranges for maximum methane (CH4) and nitrous oxide (N2O) emissions were 7.5-8.5 and 6.5-8.5, respectively, and the slurry under pH 7.5-8.5 showed the highest GHG emissions. Acidification to pH 5.5 helped reduce the CH4, N2O, and total GHG emissions by 98.0%, 29.3%, and 81.7%, respectively; while alkalinization to pH 10.0 helped achieve the mitigation effects of 74.1%, 24.9%, and 30.6%, respectively. The Pearson's correlation factor between CH4 and the gene copy of mcrA under different pH values was 0.744 (p < 0.05). Meanwhile, the correlation factors between N2O and the gene copies of amoA, narG, and nirS were 0.644 (p < 0.05), 0.719 (p < 0.05), and 0.576 (p = 0.081), respectively. The gene copies of mcrA, amoA, narG, and nirS were maintained at the lowest level under pH 5.5. These results recommended keeping slurry pH lower than 5.5 with lactic acid can help control GHG and NH3 emissions simultaneously and effectively.

Effects of self-produced lactic fermentation (SPLF) on GHG and VSC emissions during swine slurry storage.

Self-produced lactic fermentation (SPLF) is a new valued utilization technology, but its impact on gas emission remains unclear. The objective of this study is to investigate the effect of replacing the H2SO4 additive with SPLF on greenhouse gas (GHG), and volatile sulfur compound (VSC) emissions from swine slurry storage in a laboratory-scale study. In this study, SPLF is directed toward producing lactic acid (LA) through the anaerobic fermentation of slurry and apple waste under the most suitable conditions, with the LA concentration kept at 10,000-52000 mg COD/L and the pH remaining within 4.5 during the following 90 days of slurry storage. Compared with that in the slurry storage treatment (CK), the GHG emissions decreased by 86% and 87% in the SPLF and H2SO4 groups, respectively. The low pH (i.e., less than 4.5) inhibited the growth of Methanocorpusculum and Methanosarcina and resulted in very low mcrA gene copies in the SPLF group, leading to a reduction in CH4 emissions. The methanethiol, dimethyl sulfide, dimethyl disulfide, and H2S emissions in the SPLF group were reduced by 57%, 42%, 22%, and 87% and increased by 2206%, 61%, 173%, and 1856% in the H2SO4 group, respectively. Therefore, SPLF can be an innovative bioacidification technology for effectively reducing GHG and VSC emissions from animal slurry storage.

Control of odor emissions from livestock farms: A review.

Odor emission seriously affects human and animal health, and the ecological environment. Nevertheless, a systematic summary regarding the control technology for odor emissions in livestock breeding is currently lacking. This paper summarizes odor control technology, highlighting its applicability, advantages, and limitations, which can be used to evaluate and identify the most appropriate methods in livestock production management. Odor control technologies are divided into four categories: dietary manipulation (low-crude protein diet and enzyme additives in feed), in-housing management (separation of urine from feces, adsorbents used as litter additive, and indoor environment/manure surface spraying agent), manure management (semi-permeable membrane-covered, reactor composting, slurry cover, and slurry acidification), and end-of-pipe measures for air treatment (wet scrubbing of the exhaust air from animal houses and biofiltration of the exhaust air from animal houses or composting). Findings of this paper provide a theoretical basis for the application of odor control technology in livestock farms.

A clinical study on the nutritional status of patients with locally advanced rectal cancer during chemo-radiotherapy.

BACKGROUND: Rectal cancer is one of the most common gastrointestinal malignancies, and most cases include locally advanced cancers at the time of diagnosis (stage II/III). OBJECTIVES: The purpose of this study is to observe the dynamic changes in the nutritional status of patients with locally advanced rectal cancer during concurrent radiation therapy and chemotherapy and to evaluate the nutritional risk and incidence of malnutrition in these patients. METHODS: A total of 60 patients with locally advanced rectal cancer were enrolled in this study. The 2002 Nutritional Risk Screening and Patient-Generated Subjective Global Assessment Scales (PG-SGA) were used to assess nutritional risk and status. The European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire (QLQ) - C30 and QLQ-CR38 scales were used for the quality of life evaluation. Toxicity was evaluated using the CTC 3.0 standard. RESULTS: The incidence of nutritional risk among these 60 patients was 38.33% (23 of 60) before and 53% (32 of 60) after concurrent chemo-radiotherapy. There were 28 patients in the well-nourished group, with a PG-SGA score of <2 points, and 17 patients in the nutrition-changed group, with a PG-SGA score of <2 points before and 2 points during and after chemo-radiotherapy. In the well-nourished group, the incidence of nausea, vomiting and diarrhea mentioned in the summary was lower and the expectations for the future (according to the QLQ-CR30 and QLQ-CR28 scales) were higher than in the undernourished group. The undernourished group required delayed treatment more often and experienced nausea, vomiting and diarrhea earlier and for longer than the well-nourished group. These results show that the quality of life of the well-nourished group was better. CONCLUSIONS: There is a degree of nutritional risk and deficiency in patients with locally advanced rectal cancer. Chemoradiotherapy increases the incidence of nutritional risk and deficiencies. KEY WORDS: Enteral nutrition, Colorectal neoplasms, Quality of life, Chemo-radiotherapy, EORTC.

Seasonal variation in antibiotic resistance genes and bacterial phenotypes in swine wastewater during three-chamber anaerobic pond treatment.

Antibiotic resistance is a global public health concern. Antibiotic usage in pigs makes swine wastewater (SW) a reservoir for antibiotic resistance genes (ARGs). SW is usually stored and treated in a three-chamber anaerobic pond (3-CAP) in medium and small pig farms in northern China. However, the yet unexplored presence of ARGs in SW during 3-CAP treatment may result in ARGs spreading into the environment if farmers apply SW to farmland as a liquid organic fertilizer. This study investigated the profiles of and changes in ARGs in SW during its treatment in 3-CAP over four seasons and analyzed the correlation between ARGs and bacterial phenotypes, along with the physicochemical parameters of the water. The results revealed that ARG abundance decreased considerably after 3-CAP treatment in April (47%), October (47%), and December (62%) but increased in May (43%) and August (73%). The ARG copies in the influent and other SW samples increased significantly from 107 copies/mL in April to 109 copies/mL in October and were maintained in December. The increase in ARG abundance was not as rapid as the growth of the bacterial population, resulting in lower relative abundance in October and December. Bacterial communities possessed more sul1 and tetM genes, which were also positively correlated with mobile genetic elements. After the 3-CAP treatment, 16% of antibiotics and 60% of heavy metals were removed, and both had a weak correlation with ARGs. Predicted phenotypes showed that gram-positive (G+) and gram-negative (G-) bacteria have different capacities for carrying ARGs. G+ bacteria carry more ARGs than G- bacteria. This study revealed the persistence of ARGs in SW after 3-CAP treatment over different seasons. Applying SW in the proper month will mitigate ARG dissemination to the environment.

Microbial composition play the leading role in volatile fatty acid production in the fermentation of different scale of corn stover with rumen fluid.

Rumen fluid is a natural and green biocatalyst that can efficiently degrade biomass into volatile fatty acid (VFA) used to produce value-added materials. But the essence of high degradation efficiency in the rumen has not been fully analyzed. This study investigated the contribution of substrate structure and microbial composition to volatile fatty acid production in the fermentation of corn stover. The ball milled corn stover were innovatively applied to ferment with the rumen fluid collected at different digestion times. Exogeneous cellulase was also added to the ruminal fermentation to further reveal the inner mechanism. With prolonged digestion time, the microbial community relative abundance levels of Bacteroidetes and Firmicutes increased from 29.98% to 72.74% and decreased from 51.76% to 22.11%, respectively. The highest VFA production of the corn stover was achieved via treatment with the rumen fluid collected at 24 h which was up to 9508 mg/L. The ball milled corn stover achieved high VFA production because of the more accessible substrate structure. The application of exogenous cellulase has no significant influence to the ruminal fermentation. The microbial community abundance contributed more to the VFA production compared with the substrate structures.

Structure and stability characterization of pea protein isolate-xylan conjugate-stabilized nanoemulsions prepared using ultrasound homogenization.

Preparation of pea protein isolate-xylan (PPI-X) conjugate-stabilized nanoemulsions using ultrasonic homogenization and the corresponding structure and environmental stability were investigated in this study. Conditions used to prepare nanoemulsions were optimized using a response surface methodology as follows: protein concentration 8.86 mg/mL, ultrasound amplitudes 57 % (370.5 W), and ultrasound time 16 min. PPI-X conjugate-stabilized nanoemulsions formed under these conditions exhibited less mean droplet size (189.4 ± 0.45 nm), more uniform droplet distribution, greater absolute value of zeta-potential (44.8 ± 0.22 mV), and higher protein adsorption content compared with PPI-stabilized nanoemulsions. PPI-X conjugate-stabilized nanoemulsions also exhibited even particle distribution and dense network structure, which might be reasons for the observed high interfacial protein adsorption content of conjugate-stabilized nanoemulsions. Moreover, better stability against environmental stresses, such as thermal treatment, freeze-thaw treatment, ionic strength and type, and storage time was also observed for the conjugate-stabilized nanoemulsions, indicating that this type of nanoemulsions possess a potential to endure harsh food processing conditions. Therefore, results provide a novel approach for the preparation of protein-polysaccharide conjugate-stabilized nanoemulsions to be applied as novel ingredients to meet special requirements of processed foods.

[Characterization of VOCs Emissions from Caged Broiler House in Winter].

Volatile organic compound (VOCs) emissions from poultry and livestock facilities affect the surrounding environmental quality and human health. However, VOCs emissions from broiler houses have been less characterized, and studies of related dominant odorants, carcinogenic risk, and ozone formation potential are still lacking. To fill this research gap, VOCs pollutants emitted from a broiler house were investigated in this study. The VOCs emission characteristics of the broiler house during three different periods of broiler growth (early, middle, and later) were analyzed using gas chromatography-mass spectrometry. The results showed that 77 types of VOCs were detected, including 16 types of halogenated hydrocarbons, 21 types of alkanes, 5 types of olefins, 12 types of aromatic hydrocarbons, 15 types of oxygenated volatile organic compounds (OVOCs), and 8 types of sulfides. During the entire 42-day growth period, the concentrations of halogenated hydrocarbons, alkanes, olefin, aromatic hydrocarbons, and OVOCs in the broiler house showed few changes. However, with the growth of broilers, the intake of sulfur-containing amino acids and the fecal emission coefficient increased, resulting in the gradual conversion of the VOCs to sulfide. Therefore, emissions of sulfur-containing VOCs increased in the early and middle growth periods. Moreover, the increase in ventilation in the house during the later growth period resulted in a decrease in the sulfur-containing VOCs concentrations. The dominant odorants in the broiler house were naphthalene, ethyl acetate, acetaldehyde, carbon disulfide, dimethyl disulfide, methanethiol, methanethiol, and thiophene. Methanethiol had the highest odorous values, ranging from 2172.4 to 19090.9. Meanwhile, there were acceptable levels of carcinogenic risk in the early and middle growth periods, with a lifetime cancer risk (LCR) of 7.7×10-6 and 4.5×10-6, respectively. The average ozone formation potential (OFP) was (1458.9±787.4) µg·m-3. The results of this study can provide a scientific basis for the monitoring of malodorous substances and formulation of emission reduction strategies in broiler production.