Effect of UV pretreatment on the source control of floR during subsequent biotreatment of florfenicol wastewater.

UV photolysis has been recommended as an alternative pretreatment method for the elimination of antibacterial activity of antibiotics against the indicator strain, but the pretreated antibiotic intermediates might not lose their potential to induce antibiotic resistance genes (ARGs) proliferation during subsequent biotreatment processes. The presence of florfenicol (FLO) in wastewater seriously inhibits the metabolic performance of anaerobic sludge microorganisms, especially the positive correlation between UV irradiation doses and ATP content, while it did not significantly affect the organics utilization ability and protein biosynthetic process of aerobic microorganisms. After sufficient UV pretreatment, the relative abundances of floR from genomic or plasmid DNA in subsequent aerobic and anaerobic biotreatment processes both decreased by two orders of magnitude, maintained at the level of the groups without FLO selective pressure. Meanwhile, the abundances of floR under anaerobic condition were always lower than that under aerobic condition, suggesting that anaerobic biotreatment systems might be more suitable for the effective control of target ARGs. The higher abundance of floR in plasmid DNA than in genome also indicated that the potential transmission risk of mobile ARGs should not be ignored. In addition, the relative abundance of intI1 was positively correlated with floR in its corresponding genomic or plasmid DNA (p < 0.05), which also increased the potential horizontal transfer risk of target ARGs. This study provides new insights into the effect of preferential UV photolysis as a pretreatment method for the enhancement of metabolic performance and source control of target ARGs in subsequent biotreatment processes. KEY POINTS: • Sufficient UV photolytic pretreatment efficiently controlled the abundance of floR • A synchronous decrease in abundance of intI1 reduced the risk of horizontal transfer • An appreciable abundance of floR in plasmid DNA was a potential source of total ARGs.

Source preventing mechanism of florfenicol resistance risk in water by VUV/UV/sulfite advanced reduction pretreatment.

To avoid the inhibition of microbial activity and the emergence of bacterial resistance, effective abiotic pretreatment methods to eliminate the antibacterial activity of target antibiotics before the biotreatment system for antibiotic-containing wastewater are necessary. In this study, the VUV/UV/sulfite system was developed as a pretreatment technique for the source elimination of florfenicol (FLO) resistance risk. Compared with the VUV/UV/persulfate and sole VUV photolysis, the VUV/UV/sulfite system had the highest decomposition rate (0.33 min‒1) and the highest defluorination (83.0%), resulting in the efficient elimination of FLO antibacterial activity with less than 2.0% mineralization, which would effectively retain the carbon sources for the sludge microorganisms in the subsequent biotreatment process. Furthermore, H• was confirmed to play a more important role in the elimination of FLO antibacterial activity by controlling the environmental conditions for the formation and transformation of reactive species and adding their scavengers. Based on the theoretical calculation and proposed photolytic intermediates, the elimination of FLO antibacterial activity was achieved by dechlorination, defluorination and removal of sulfomethyl groups. When the pretreated FLO-containing wastewater entered the biological treatment unit, the abundance of associated antibiotic resistance genes (ARGs) and the relative abundance of integrons were efficiently prevented by approximately 55.4% and 22.9%, respectively. These results demonstrated that the VUV/UV/sulfite system could be adopted as a promising pretreatment option for the source elimination of FLO resistance risk by target decomposition of its responsible structures before the subsequent biotreatment process.