Assuntos
Encéfalo , Curcumina , Fator 2 Relacionado a NF-E2 , Animais , Curcumina/farmacologia , Fator 2 Relacionado a NF-E2/metabolismo , Fator 2 Relacionado a NF-E2/genética , Ratos , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Masculino , Ratos Sprague-Dawley , Explosões , Doenças Neuroinflamatórias/metabolismo , Doenças Neuroinflamatórias/tratamento farmacológico , Heme Oxigenase-1/metabolismo , Heme Oxigenase-1/genética , Traumatismos por Explosões/metabolismo , Transdução de Sinais/efeitos dos fármacos , Heme Oxigenase (Desciclizante)/metabolismo , Anti-Inflamatórios não Esteroides/farmacologiaAssuntos
Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Ácidos Ftálicos/toxicidade , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Colesterol/sangue , Cromatografia Líquida , Lipoproteínas HDL/sangue , Fígado/enzimologia , Masculino , Espectrometria de Massas , Metabolômica , Distribuição Aleatória , Ratos , Ratos Wistar , Triglicerídeos/sangueRESUMO
OBJECTIVE: Di-(2-ethylhexyl) phthalate (DEHP) is a ubiquitous environmental contaminant. As an endocrine disruptor, it seriously threatens human health and ecological environmental safety. This study examines the impact of intervention with soybean isoflavones (SIF) on DEHP-induced toxicity using a metabonomics approach. METHODS: Rats were randomly divided into control (H), SIF-treated (A, 86 mg/kg body weight), DEHP-treated (B, 68 mg/kg), and SIF plus DEHP-treated (D) groups. Rats were given SIF and DEHP daily through diet and gavage, respectively. After 30 d of treatment, rat urine was tested using UPLC/MS with multivariate analysis. Metabolic changes were also evaluated using biochemical assays. RESULTS: Metabolomics analyses revealed that p-cresol glucuronide, methyl hippuric acid, N1-methyl-2-pyridone-5-carboxamide, lysophosphatidycholine [18:2 (9Z, 12Z)] {lysoPC [18:2 (9Z, 12Z)]}, lysoPC (16:0), xanthosine, undecanedioic acid, and N6-acetyl-l-lysine were present at significantly different levels in control and treatment groups. CONCLUSION: SIF supplementation partially protects rats from DEHP-induced metabolic abnormalities by regulating fatty acid metabolism, antioxidant defense system, amino acid metabolism, and is also involved in the protection of mitochondria.
Assuntos
Dietilexilftalato/toxicidade , Disruptores Endócrinos/toxicidade , Poluentes Ambientais/toxicidade , Glycine max/química , Isoflavonas/urina , Metaboloma , Substâncias Protetoras/metabolismo , Animais , Feminino , Plastificantes/toxicidade , Ratos , Ratos Wistar , UrináliseRESUMO
Galectin-3, a unique chimera-type member of the ß-galactoside-binding soluble lectin family, is present in both normal and cancer cells and plays a crucial role in the regulation of cell adhesion. It is involved both in accelerating detachment of cells from primary tumor sites and promoting cancer cell adhesion and survival to anoikis in the blood stream. Cell adhesion molecules (CAMs) are membrane receptors that mediate cell-cell and cell-matrix interactions, and are essential for transducing intracellular signals responsible for adhesion, migration, invasion, angiogenesis, and organ-specific metastasis. This review will discuss the recent advances in our understanding the biological functions, mechanism and therapeutic implication of the interaction between galectin-3 and CAMs in cancer metastasis.