[The role of the nervous factor in regulation of oxygen supply to the small intestine]. / Znachenie nervnogo faktora v reguliatsii kislorodnogo snabzheniia tonkoi kishki.

The experimental model of rat bilateral subdiaphragmatic stem vagotomy was used to study the role of parasympathetic nervous system in regulation of circulation and oxygen supply to the small intestine. Cut of the vagus nerves is shown to cause redistribution of the blood flow between gastroduodenal organs, to slow down local circulation, to reduce oxygen tension in the muscular lining of the jejunum, to raise water content in the wall and change magnetorelaxational characteristics. Morphologically this manifested in altered arteriolar and capillary configuration, dilation of the capacity microvessels, dystrophia of microvessel wall, red cell aggregation in capillaries and venules, defective permeability of the vascular wall. The above indices changed most 7 and 30 days after vagotomy. It is evident that impaired vagus innervation produces secondary circulation hypoxia in the small intestine.

Changes in pancreahepatoduodenal organs during experimental acute lipogenic pancreatitis.

Experiments on animals with experimental lipogenic pancreatitis showed that disturbances in lipid metabolism stimulate destructive processes in the pancreas, liver, and spleen. Acute experimental lipogenic pancreatitis is characterized by extensive destructive changes in the pancreatic parenchyma accompanied with fatty necrobiosis of hepatocytes, fatty transformation of the gallbladder wall, and pronounced reaction of the reticuloendothelial stroma in the spleen.

[The histophysiological characteristics of ovarian development with an altered level of thymic peptides at infancy in mice]. / Gistofiziologicheskie osobennosti razvitiia iaichnika v usloviiakh izmeneniia soderzhaniia timicheskikh peptidov v infantil'nom vozraste u myshei.

Thymic peptides effect on the female gonad development was studied: consequences of these substances dysbalance in infancy (T-activine administration of 0.1 microgram/g of body weight in dose since 8 to 21 postnatal day) was analysed. Morphofunctional diagnostics of the ovarian state is based on the spontaneous organism reaction to the drug administration (to 21-days-old animals) and on the distant consequences of its activity (in 1-, 2-, 3-months female animals). Thymic peptides dysbalance in infancy promotes only transitory disorders of the ovarian histophysiology, expressing in transitory inhibition of the follicle genesis with the simultaneous atretic process activation, which results in puberty delay with the following complete restoration of reproductive function. Taking into account that in the early neonatal period gonad reacts to T-activine administration with the development of strong ovarian pathology, the results obtain allow to conclude that the reactivity to thymic peptides content oscillations reduces so as the significance of the factors cited in the ovarian morphogenesis regulation in proportion with the animals' maturity.

[Carbohydrate determinants of organs of the reproductive tract of the mouse according to the results of using lectins with different specificities]. / Uglevodnye determinanty organov reproduktivnoi sistemy myshi po dannym ispol'zovaniia lektinov razlichnoi spetsifichnosti.

Histotopography of lectin receptor sites in adult mice ovary, oviduct, uterus, testis and epididymis has been investigated on light-optic level by means of lectin-peroxidase technique. Paraffin sections are treated with peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA) and Laburnum anagyroides lectin (LAL), conjugated with horseradish peroxidase. Concanavalin A (Con A) receptor sites are visualized by indirect method. The usefulness of lectins for selective histochemic evaluation of definite organ structures is demonstrated. Zona pellucida, luteocytes, oviductal and uterine epitheliocytes are rich in receptor sites for all lectin used in the investigation. The most intense binding to zonae pellucidae glycoconjugates possess WGA and LAL, to luteocytes--PNA, SBA and LAL, to oviductal and uterine epitheliocytes--Con A and LAL. The preferential SBA binding to the acrosomal system and plasma membranes of spermatogenic cells is demonstrated. Changes in lectin-binding patterns during the maturation of intraovarian oocytes and spermatogenic cells are also studied. The perspectives of practical application of the results obtained, as well as trends in further lectin histochemistry investigations of the reproductive system are discussed.