[Influence of ethanol and ethanol-induced lipid peroxidation on the steroidogenic activity of testicles]. / Vliianie étanola i indutsiruemogo im perekisnogo okisleniia lipidov na steroidogennuiu aktivnost' semennikov.

Chronic alcohol intoxication results in the diminished testosterone level and activation of lipid peroxidation in rat testes. A significant decrease in 3 beta-hydroxysteroid-hydrogenase and 17-dehydrogenase activity has been observed in microsomes. Analogous shifts have been evoked in vitro by the induction of lipid peroxidation in microsomes. That permitted one to suppose that alcohol-induced alterations in tests are partially mediated by induction of lipid peroxidation.

[Effect of acute alcohol intoxication on lipid peroxidation in testis and adrenal glands of rats]. / Vliianie ostroi alkogolnoi intoksikatsii na protsessy perekisnogo okisleniia lipidov v semennikakh i nadpochechnikakh krys.

Hormones level and lipid peroxidation processes under influence of acute alcohol intoxication are tested in testes and adrenals of rats. Ethanol marker effects--the rise of corticosterone biosynthesis and depression of testosterone concentration--were reproduced in the experiment. At the moment of maximal changes in steroid levels indices characterising lipid peroxidation didn't differ from the control. At the early stage of the experiment transient shifts in malonic dialdehyde and dienic conjugates levels were noted. The data obtained does not agree with the hypothesis of acute ethanol effects in testes and adrenals being mediated through the changes of lipid peroxidation rate.

[Corticosterone level in testes of rats in varying adrenal functional activity]. / Uroven' kortikosterona v semennikakh krys pri razlichnoi funktsional'noi aktivnosti nadpochechnikov.

The presence of corticosterone in rat testes was demonstrated by combining HPLC and RIA techniques. The presence of the hormone was also taken into account. The testicular corticosterone content was shown to depend upon the functional activity of the adrenals and to diminish acutely after adrenalectomy. Analysis of correlation between the concentration of the hormones in the plasma and testes has shown corticosterone to be formed by testicular cells.

[Effect of alpha-tocopherol on adrenal cortex functions under stress]. / Vliianie alpha-tokoferola na funktsiiu kory nadpochechnikov pri stresse.

alpha-Tocopherol has been studied for its effect on lipid peroxidation and steroidogenesis in the adrenal cortices of rat and rabbit under stress. The vitamin is shown to exert an inhibitory effect on the lipid peroxidation developing under chronic stress. A biphasic pattern of the alpha-tocopherol effect on the steroidogenesis in the adrenal cortex is established: a decrease in the release of the steroids under the acute stress and maintaining of their levels under the chronic stress. A conclusion is drawn about a potential alpha-tocopherol application to correct the adrenal cortex function under stress.

[Content of biological antioxidants and lipid peroxidation in the adrenal cortex in experimental circulatory insufficiency]. / Soderzhanie biologicheskikh antioksidantov i perekisnoe okislenie lipidov v kore nadpochechnikov pri éksperimental'noi nedostatochnosti krovoobrashheniia.

Thirteen-week experimental insufficiency in rats causes exhaustion of adrenal cortex function. The number of diene conjugates in the adrenals increased, the alpha-tocopherol level decreased. It is suggested that reduced level of antioxidant protection and the associated activation of lipid peroxidation processes underlie the disorders of adrenal cortex function.

[Dynamics of lipid peroxidation and steroidogenesis in adrenal cortex during stress]. / Dinamika protsessov perekisnogo okisleniia lipidov i steroidogeneza v kore nadpochechnikov pri stresse

The phase character of lipid peroxidation has been found in the rabbit adrenal cortex in the process of adaptation to extreme loads. Under acute stress the activation of lipid peroxidation is directly dependent on the hormonal synthesis processes. Under conditions of the prolonged stress factor an enhancement of the lipid peroxidation intensity in the adrenal cortex coincides with a decrease in the steroidogenesis rate.

[Lipid peroxidation in the adrenal cortex during exhausting stress]. / Perekisnoe okislenie lipidov v kore nadpochechnikov pri istoshchaiushchem stresse.

Under prolonged stress which is connected with exhaustion of functional resources of adrenal cortex the activation of lipid peroxidation processes in this gland was found. It is possible that the reason for such lipid peroxidation activation is the decrease in the content of adrenal cortex ascorbic acid and alpha-tocopherol.

[Comparative determination of the potential for activating lipid peroxidation of the adrenal cortex, liver and heart in various animals]. / Sravnitel'noe opredelenie potentsial'noi vozmozhnosti aktivatsii perekisnogo okisleniia lipidov v kore nadpochechnikov, pecheni i serdtse razlichnykh zhivotnykh.

Studies have been made on the rate of spontaneous, ascorbate- and NADP.H-dependent lipid peroxidation in the adrenal cortex, liver heart of rats, rabbits and cattle. It was shown that in spite of some species differences in predisposition of tissues to lipid peroxidation. The highest rate of this process is characteristic of the liver of all the animals investigated.

[Activation of lipid peroxidation in the adrenal cortex by metal ions]. / Aktivatsiia perekisnogo okisleniia lipidov v kore nadpochechnikov ionami metallov.

The processes of lipid peroxidation have been studied in bovine adrenal cortex in vitro. The lipid peroxidation rate in this tissue is shown to be dependent on the content of metal ions. EDTA, deferroxamine and penicyllamine inhibit spontaneous lipid peroxidation by 25, 50 and 42%, respectively. The ability to activate the process permits arranging metal ions in the following sequence: Fe2+ greater than Fe3+ greater than Cu2+ greater than Mg2+ greater than Mn2+. The maximum activation of lipid peroxidation is observed at Fe2+ and Fe3+ concentrations within the range of 5 x 10(-6) x 10(-4) M.

[Palmitoyl-CoA-dehydrogenase from rabbit adrenals, liver and myocardium]. / Pal'mitoil-KoA-degidrogenaza nadpochechnikov, pecheni i serdtsa krolika.

Partially purified preparations of palmitoyl-CoA dehydrogenase from rabbit adrenal glands, liver and heart tissues exhibited similar kinetic parameters. Km value constituted 6.58, 5.26 and 6.67 microM for the enzyme from adrenal glands, liver and heart tissues, respectively. At the same time, palmitoyl-CoA dehydrogenase possessed lower catalytic capacity in adrenal glands due to the decreased amount of the enzyme as compared with that of liver or heart tissues.

[Oxidation of [1-14C]palmitoyl-CoA, [1-14C]acetyl-CoA and [2-14C]pyruvate in rabbit adrenal, liver and heart mitochondria under normal conditions and in acute stress]. / Okislenie [1-14C]pal'mitoil-CoA, [1-14C]atsetil-CoA i [2-14C]piruvata v mitokhondriiakh nadpochechnikov, pecheni i serdtsa krolikov v norme i pri ostrom stresse.

The acute immobilized stress was studied for its effect on oxidation rate of [1-14C]palmitoyl-CoA, [1-14C]acetyl-CoA and [2-14C]pyruvate in mitochondria of the adrenals, liver and heart of rabbits. The stress effect on the energy metabolism of adrenals is associated with an increase of the rate of CO2 formation from pyruvate and with a decrease of the rate of CO2 formation from palmitoyl-CoA. Intensified oxidation of all substrates is observed in the heart mitochondria. The processes of beta-oxidation are more active in the liver. The data obtained evidence for differences in the mechanisms of energy metabolism reconstruction under acute stress in tissues with different functional specialization.

[Effect of acute stress on the fatty acid composition of lipids of adrenal and heart mitochondria in rabbits]. / Vliianie ostrogo stressa na zhirno-kislotnyi sostav lipidov mitokhondrii nadpochechnikov i serdtsa krolika.

A comparative study of changes in the fatty acid composition of rabbit heart and adrenal mitochondria was carried out after acute (1h) immobilization stress. In heart mitochondria the stress induced a decrease in the content of capric, lauric, myristic and pentadecanic acids. A statistically significant reduction of the amount of heptadecanoic, linoleic, arachidonic acids and an increase in the level of palmitic acid was noted in adrenal mitochondria. The acute stress resulted in differently directed shifts in the saturation of fatty acids. An elevation of the sum of unsaturated fatty acids was observed in the heart, and a decrease was detected in the adrenal glands. The above shifts in the fatty acid composition of adrenal and heart mitochondria provide evidence for different directions of lipid metabolism in these organs following stress.

[Oxidation of different substrates in mitochondria of bovine and rat adrenal cortex]. / Okislenie razlichnykh substratov v mitokhondriiakh kory nadpochechnikov byka i krys.

The rate of oxygen uptake by mitochondria of bovine and rat adrenals was determined during oxidation of the Krebs cycle substrates. Succinate is established to be oxidized most actively under in vitro conditions.