Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
Arch Virol ; 164(12): 3059-3063, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31549301

RESUMO

Swine are the only known hosts of swinepox virus (SWPV), the sole member of the genus Suipoxvirus, family Poxviridae. Rapid diagnosis is recommended for appropriate interventions because of the high morbidity associated with this virus. This study describes a real-time quantitative PCR (qPCR) assay for rapid detection and quantification of SWPV. The detection limit, repeatability, reproducibility, and specificity of this assay were determined. The efficiency was 96%, and the R2 value was 0.996. The detection limit was 1 fg or 10-0.5 TCID50/50 µL. Tests showed that the greatest source of error in the SWPV qPCR assay was variation between analysts rather than different qPCR kits or equipment. All nucleic acids from other viruses or samples collected from swine were negative in the specificity test. qPCR for SWPV is a new method with tested variables that allows main sources of error in laboratory diagnosis and viral quantification to be identified.


Assuntos
Infecções por Poxviridae/diagnóstico , Suipoxvirus/genética , Doenças dos Suínos/virologia , Animais , DNA Viral/genética , Limite de Detecção , Infecções por Poxviridae/veterinária , Reação em Cadeia da Polimerase em Tempo Real , Suipoxvirus/classificação , Suipoxvirus/isolamento & purificação , Suínos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA