RESUMO
Numerous novel anaerobic bacteria were isolated from the crevicular spaces of dogs with periodontitis. The phenotypic characteristics of these bacterial isolates indicated that they were similar to members of the genus Porphyromonas. However, comparison of the 16S rRNA gene sequences of the isolates indicated that they were related to members of the Bacteroides splanchnicus subgroup. A representative of the novel isolates, strain B106(T), induced alveolar bone loss in a mouse model of experimental periodontal disease. Based on biochemical, morphological, molecular phylogenetic, and pathogenic evidence, it is proposed that the taxonomic subgroup containing these novel isolates and B. splanchnicus should be classified in a new genus, Odoribacter gen. nov., within the family 'Porphyromonadaceae'. In addition, it is proposed that B. splanchnicus should be reclassified as Odoribacter splanchnicus comb. nov., and that the newly identified isolates should be classified as representing Odoribacter denticanis sp. nov., the type strain of which is B106(T) (=ATCC PTA-3625(T)=CNCM I-3225(T)).
Assuntos
Bacteroides/classificação , Bacteroidetes/classificação , Bacteroidetes/isolamento & purificação , Doenças do Cão/microbiologia , Bolsa Periodontal/microbiologia , Periodontite/veterinária , Perda do Osso Alveolar/microbiologia , Animais , Técnicas de Tipagem Bacteriana , Bacteroides/genética , Bacteroidetes/genética , Bacteroidetes/patogenicidade , DNA Bacteriano/análise , Cães , Genes de RNAr , Humanos , Camundongos , Dados de Sequência Molecular , Periodontite/microbiologia , Fenótipo , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
The etiology of human periodontal disease has been the focus of considerable research, yet relatively little is known about the causative agents of companion animal periodontitis. In humans, Porphyromonas gingivalis, a black-pigmented anaerobic bacteria (BPAB), has been implicated as the primary periopathogen. It has been demonstrated that BPAB are also found in companion animal periodontal pockets. While some animal BPAB have been individually identified, a study to identify the most frequently isolated subgingival BPAB has not been completed using genetic tools. The objective of this work was to identify the types and relative frequencies of pigmented anaerobic bacteria found in the periodontal pockets of dogs. Porphyromonas salivosa, Porphyromonas denticanis (a novel species) and Porphyromonas gulae were found to be the most frequently isolated BPAB associated with canine periodontitis.