RESUMO
Most molecular studies on the visual system in fish have been performed on freshwater teleosts such as goldfish and zebrafish where cones and rods appear simultaneously. Many marine fishes have long larval phase in the upper pelagic zone before transformation into a juvenile and a benthic life style. The retina at the larval stages consists of only single cone cells; later during metamorphosis double cones and rods develop. The flatfish Atlantic halibut (Hippoglossus hippoglossus) is a typical example of a marine species with such a two-step retina development. In this study, we have cloned five different opsins from Atlantic halibut larvae and juvenile retinas. Sequence comparisons with other opsins and phylogenetic analysis show that the five genes belong to the opsins of long-wavelength sensitive (L); middle-wavelength sensitive, M(Cone) and M(Rod); and short-wavelength sensitive, S(Blue) and S(Ultraviolet), respectively. In situ hybridization analysis reveals expression in double cone (L and M(Cone)), single cone (S(Blue) and S(Ultraviolet)), and rod (M(Rod)) types of photoreceptor cells in juvenile halibut retina. The visual system in Atlantic halibut seems therefore to have all four types of cone photoreceptors in addition to rod photoreceptors. This work shows for the first time molecular isolation of a complete set of retinal visual pigment genes from a marine teleost and describes the first cloning of an ultraviolet-sensitive opsin type from a marine teleost.
Assuntos
Linguado/genética , Células Fotorreceptoras de Vertebrados/química , Opsinas de Bastonetes/genética , Sequência de Aminoácidos , Animais , Clonagem Molecular , DNA/análise , Hibridização In Situ , Dados de Sequência Molecular , Células Fotorreceptoras de Vertebrados/metabolismo , Reação em Cadeia da Polimerase , Opsinas de Bastonetes/metabolismo , Homologia de Sequência de AminoácidosRESUMO
The Drosophila homeobox gene sine oculis and its murine homologue Six3 have both been shown to have regulatory functions in eye and brain development. In zebrafish, three Six3-related genes with conserved expression during early eye and head formation have been identified. One of these, six7, is first expressed at the gastrula stage in the involuting axial mesoderm, and later in the overlying neuroectoderm from which the forebrain and optic primordium develop. To elucidate the mechanisms regulating six7 expression, we isolated a 2.7-kb fragment of the 5'-flanking region. Three sequentially deleted fragments of this upstream region were used to produce GFP reporter constructs for analysis of tissue-specific expression in zebrafish embryos. The results show that a 625-bp upstream fragment is sufficient to direct strong expression of the reporter during gastrulation and early neurulation. The proximal part of the promoter contains binding sites for various constitutive transcription factors and an additional upstream element that was shown to be critical in directing expression to the anterior region of the zebrafish brain.
Assuntos
Proteínas de Homeodomínio/genética , Regiões Promotoras Genéticas , Proteínas de Peixe-Zebra , Peixe-Zebra/genética , Animais , Sequência de Bases , Sítios de Ligação , Proteínas de Fluorescência Verde , Proteínas Luminescentes , Dados de Sequência Molecular , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Peixe-Zebra/embriologiaRESUMO
Similar to the Drosophila homeobox gene sine oculis, several of the vertebrate homologues (Six genes) are expressed during eye formation and differentiation. In addition, most of these vertebrate genes show expression in mesodermal derivatives in adults and/or earlier stages of development. We have identified a zebrafish (Danio rerio) gene, six8, which shows the greatest similarity to murine Six4. The deduced proteins of these two genes have an overall sequence identity of 41%, while the homeodomains and Six domains are highly conserved, 90% and 81%, respectively. The spatiotemporal expression pattern of six8 was analyzed by RT-PCR and in situ hybridization. Transcripts were detected in a wide range of embryonic stages and in adults. Notably, the strongest expression was observed in head mesoderm of late gastrula and early neurula stages.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Homeodomínio/genética , Mesoderma/metabolismo , Proteínas do Tecido Nervoso/genética , Transativadores , Proteínas de Peixe-Zebra , Peixe-Zebra/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Primers do DNA , Embrião não Mamífero/fisiologia , Indução Embrionária , Cabeça , Proteínas de Homeodomínio/química , Hibridização In Situ , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/química , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Transcrição Gênica , Peixe-Zebra/embriologiaRESUMO
Both the Drosophila homeobox gene sine oculis and its murine homologue Six3 have regulatory functions in eye development. In zebrafish, in addition to two previously reported homologues of murine Six3, we have identified a related gene (six7). Although the deduced Six7 protein shares less than 68% sequence identity with the other known zebrafish Six3-like proteins, the embryonic expression patterns have highly conserved features. The six7 transcripts are first detected in involuting axial mesendoderm and, subsequently, in the overlying neurectoderm from which the forebrain and optic primordia develop. Similar to the two other zebrafish Six3 homologues, the expression boundaries of six7 correspond quite closely with the edges of the optic vesicles. Hence, the partially overlapping expression domains of these three six genes probably contribute to anteroposterior specification and in defining the eye primordia.