Agaricus bisporus compost improves the potential of Salix purpurea × viminalis hybrid for copper accumulation.

The aim of the study was to determine the ability of spent mushroom compost (SMC) from the production of Agaricus bisporus (A. bisporus) to stimulate the growth and efficiency of copper (Cu) accumulation by Salix purpurea × viminalis hybrid. Roots, shoots and leaves were analysed in terms of total Cu content and selected biometric parameters. Due to the absence of information regarding the physiological response of the studied plant, low molecular weight organic acids (LMWOAs), phenolic compounds and salicylic acid (SA) contents were investigated. The obtained results clearly demonstrate the effectiveness (usefulness) of SMC in promoting the growth and stimulation of Cu accumulation by the studied Salix taxon. The highest Cu content in roots and shoots was found at the 10% SMC addition (507±22 and 380±11 mg kg(-1) DW, respectively), while there was a reduction of the content in leaves and young shoots (109±8 and 124±7 mg kg(-1) DW, respectively). In terms of physiological response, lowered secretion of LMWOAs, biosynthesis of phenolic compounds and SA, as well as accumulation of soluble sugars in Salix leaves was observed with SMC addition. Simultaneously, an elevation of the total phenolic content in leaves of plants cultivated with SMC was observed, considered as antioxidant biomolecules.

The problem of medical dispatchers' responsibility functioning in the emergency medical services system.

AIM OF THE STUDY: Deaths due to inappropriate functioning of the emergency medical services system, as recently described by Polish mass media, has drawn the attention of society to the activities of medical dispatchers. Legal regulations impose obligations on those persons associated with receiving phone calls and dispensing appropriate emergency medical teams. In this paper an analysis of chosen medicolegal opinions from the practice of the Department of Forensic Medicine and Forensic Toxicology, Medical University of Silesia in Katowice, towards malpractices committed by dispatchers of EMS, was performed. MATERIAL AND METHODS: The authors analysed 12 of medicolegal opinions, issued from 2007 to 2012 by a team of experts. RESULTS: The errors noted in the work of dispatchers consisted of delays in giving appropriate assistance due to the inability to properly converse, a propensity to downplay patients' symptoms, and dispatchers crossing their own competences. CONCLUSIONS: The problem may be resolved by the subsidy of EMS, fine-tuning the algorithms for conduct, and proper education of both staff and public.

Influence of Ca/Mg ratio on phytoextraction properties of Salix viminalis. II. Secretion of low molecular weight organic acids to the rhizosphere.

A hydroponic experiment in a phytotron was performed to investigate the effect of two different Ca/Mg ratios (4:1 and 1:10) and trace element ions (Cd, Cu, Pb and Zn) in solution on the efficiency of low molecular weight organic acid (LMWOA) formation in Salix viminalis rhizosphere. Depending on the Ca/Mg ratio and presence of selected trace elements at 0.5mM concentration, the amount and kind of LMWOAs in the rhizosphere were significantly affected. In physiological 4:1 Ca/Mg ratio the following complex of acids was observed: malonic (Pb, Zn), citric, lactic, maleic and succinic (Zn) acids. Under 1:10 Ca/Mg ratio, citric (Cd, Zn), maleic and succinic (Cd, Cu, Pb, Zn) acids were seen. Additionally, high accumulation of zinc and copper in all systems was observed, with the exception of those where one of the metals was at higher concentration. Summing up, the results indicate a significant role of LMWOAs in Salix phytoremediation abilities. Both effects can be modulated depending on the mutual Ca/Mg ratio.