Fulvivirga sedimenti sp.nov, isolated from the sediment of oceanic tidal zone.

A yellow-orange, rod-shaped (0.3-0.4 × 2.2-3.8 µm), Gram-stain-negative, non-motile, atrichous bacterium, designated strain 1062T, was isolated from tidal zone sediment collected from Xiaoshi Island, Weihai, Shandong Province, China. Growth of strain 1062T occurred at 15-43 °C (optimum, 37 °C), pH 6.0-9.5 (optimum, pH 7.5) and with 0.5-7.0% (w/v) NaCl (optimum, 2.0-3.0%). Nitrate was not reduced to nitrite or nitrogen. Positive for catalase and oxidase activity, as well as the hydrolysis of casein, DNA, starch and Tweens 20, 40, 60, but negative for hydrolysis of alginate, CM-cellulose, agar and Tweens 80. The 16S rRNA gene sequence comparisons indicated that strain 1062T had a sequence similarity of 96.2% with Fulvivirga lutimaris KCTC 42720T and 94.3% with Fulvivirga kasyanovii KCTC 12832T. Phylogenetic analysis showed that strain 1062T belonged to the genus Fulvivirga. The genomic DNA G + C content was 45.1 mol%. The ANI values of strain 1062T in comparison to F. kasyanovii KCTC 12832T and F. lutimaris KCTC 42720T were all lower than 70%. The dDDH values of strain 1062T in comparison to F. kasyanovii KCTC 12832T and F. lutimaris KCTC 42720T were all lower than 20%. The average amino acid identity values of strain 1062T in comparison to F. kasyanovii KCTC 12832T and F. lutimaris KCTC 42720T were all lower than 60%. The major fatty acids (> 5%) of strain 1062T were iso-C15:0, iso-C15:0 G, iso-C15:0 3OH, iso-C17:0 3-OH and summed feature 3 (C16:1ω7c/C16:1ω6c). The polar lipids consist of an aminolipid, phosphatidylethanolamine, phosphatidyglycerol and diphosphatidylglycerol. The major respiratory quinone was MK-7. Based on the results of physiological, biochemical and chemotaxonomic characteristics, the strain 1062T (= KCTC 72868T = MCCC 1H00499T) was identified as a new species of the genus Fulvivirga and the name Fulvivirga sedimenti sp. nov, is proposed.

Robertkochia sediminum sp. nov., isolated from coastal sediment.

An orange-pigmented, Gram-negative, strictly aerobic, non-flagellated, and rod-shaped bacterium, designated strain 1368T, was isolated from coastal sediment collected from Xiaoshi Island, Weihai, China. The optimal growth conditions were determined to be at 37 °C, pH 7.0 and in 2%-3% (w/v) NaCl. According to the phylogenetic analysis of the 16S rRNA gene sequence, strain 1368T showed the highest sequence similarity of 97.8% to Robertkochia marina JCM 18552T, followed by R. solimangrovi KCTC 72252T (96.5%). The DNA G + C content was 45.7% and the major fatty acids were iso-C15:0, iso-C15:1 G and iso-C17:0 3-OH. The major polar lipids were one phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The predominant respiratory quinone was menaquinone-6 (MK-6). The DNA-DNA relatedness between the genomes of strain 1368T and R. marina JCM 18552T and R. solimangrovi KCTC 72252T was 18.2% and 18.4%, and the average nucleotide identity were 70.6% and 73.8%, respectively. On the basis of the phenotypic, genetic and physiological characteristics, strain 1368T was suggested to represent a novel species of the genus Robertkochia, for which the name Robertkochia sediminum sp. nov. is proposed. The type strain is 1368T (MCCC 1H00444T = KCTC 82418T).