[Effects of the antioxidant ionol on humoral immune response in experimental influenza]. / Vliianie antioksidanta ionola na gumoral'noe zveno immunogo otveta pri éksperimental'nom grippe.

The effect of the antioxidant ionol on a humoral immune response was studied in experimental influenza. With preventive introduction of ionol, the affinity of anti-influenza G antibodies circulating in the blood stream was shown to be decreased as compared to that in control animals. The changes in the affinity during influenza infection were cyclic and less marked in the group of animals receiving ionol. The administration of the antioxidant substantially reduced the detection rate of specific blood immune complexes and contributed to the production of antibody-forming cells. The findings suggest that ionol has a favourable effect on a humoral immune response in experimental influenza and alleviates the course of an infectious process.

[Immunochemical characteristics of the population structure of antibody-producing cells in experimental influenza]. / Immunokhimicheskaia kharakteristika struktury populiatsii antiteloobrazuiushchikh kletok pri éksperimental'nom grippe.

The authors presented the results of population study of specific IgG-antibody-forming cells (AFC) in the pool of mice splenocytes performed in the time-course of simulated influenza with the help of experimental mathematical approach which permitted making a detailed observation of the functional structure of the population and analysing the changes in the spectrum of the AFC subpopulations in the time-course of viral disease. Mathematical simulation of the main stage of immunopointed identification of AFC on +nitrocellulose filters (NCF) permitted the verification of the ratio between the characteristic features of the spots on the NCF and the number and affinity of cell-secreting IgG antibodies. New data are obtained on the formation of the population of +virus-specific IgG-AFC which are featured by discreteness of the AFC +affinity ++ spectrum, cyclic changes in their activity and shifts in the stages of the AFC subpopulation activity. The authors substantiated the necessity to revise the existing theoretical models of the formation of humoral immune response to viral infections.

[Study of biological equilibrium in the body in viral infection of different degree of severity (on the model of influenza)]. / Biologicheskoe ravnovesie v organizme pri virusnoi infektsii raznoi tiazhesti (na modeli grippa).

The authors made a comparative analysis of the contribution to the outcome of the disease by realization of information of viral genome and host responses in the time-course of simulated lethal and nonlethal mice influenza. They studied the effect of ionol, pathogenetic antioxidant, prevention and depicted absolutely new features of the diseases severity and outlined possible points of effective drug application. The suggested scheme of the time-course measurement of viral involvement of the host body and the formation of its responses could be recommended in the study of mechanisms of the viral infection development and the activity of antiviral agents.

[Functional activity of antibody-producing cells in mice with experimental influenza]. / Funktsional'naia aktivnost' antiteloobrazuiushchikh kletok v dinamike éksperimental'noi grippoznoi infektsii myshei.

The paper presents the findings on the functional activity of specific antibody-forming cells (AFC) in the time-course of the primary influenza infection in mice. The concept of AFC functional activity is validated. Studying of the AFC population in the pool of splenocytes based on the analysis of functional activity permitted one to make a detailed description of specific AFC, and make a series of conclusions of the technique of AFC identification, reveal the changes in the structure of the virus-specific AFC population under the influenza infection which are not related to their quantity. Principle difference was revealed in the time-course of AFC functional activity in various patterns of influenza. Continuous changes in the AFC structure were observed during the whole period of observation.

[Study of general principles of humoral immune response in viral infection by sample analysis of the properties of specific immunoglobulins]. / Issledovanie obshchikh zakonomernostei gumora'lnogo immunnogo otveta pri virusnoi infektsii posredstvom diskretnogo analiza svoistv spetsificheskikh immunoglobulinov.

Humoral immunity in murine experimental influenza was specified using experimental mathematical modelling intended for further systemic analysis of dynamics of immunological processes. The methodology of the proposed approach was based on the choice of affinity as an indicator of the function of specific antibodies, and on the concept of discrete functional structure of the immune system. The data were obtained by calculating affinity and antibody heterogeneity indices and by evaluating the distribution of functions by affinity. The successions of active antibody subpopulations with affinities varying in the course of infection and the presence in the plasma of protein fractions capable of low-affinity binding with influenza virus in non-infected animals were demonstrated. The relationship between the detected plasma protein populations and corresponding antibody-producing cells was confirmed. The mathematical modelling results were verified by physicochemical and immunochemical methods. The mechanisms by which the observed phenomena may be realized were considered from the viewpoint of the current theory of immunity.

[Differentiated study of specific antibody populations as a possible way to increase the prognostic value of indicators of humoral immunity in influenza]. / Differentsirovannoe izuchenie populiatsii spetsificheskikh antitel kak vozmozhnyi pu't povysheniia prognosticheskoi znachimosti poka- zatelei gumora'lnogo immuniteta pri grippe.

The article overviews the literature and original research data on the ways to increase the informative value of assessed parameters of the humoral immune response to viral disease formation. The need to analyse the heterogeneity of specific IgG class antibodies by their affinity index and to determine the level of circulating specific immune complexes is discussed. It is demonstrated that the value of the humoral immunity studies in influenza-virus infection may be significantly increased by investigating not only the accumulation of circulating immunoglobulins of various classes but the antibody affinity within its class and by assessing the proportions of circulating and immune-complex-bound antibodies. This allows for considering the humoral immunity as a constituent of the interrelated and interdependent immunity mechanism complex.