RESUMO
OBJECTIVE: To determine whether 17beta-oestradiol (E2) modulates interleukin (IL) 1beta-induced proteoglycan degradation in chondrocytes, and to analyse the part played by metalloproteinases (MMPs) in this process. METHODS: Primary cultured rabbit articular chondrocytes were prepared and treated with 10 ng/ml IL1beta combined or not with 0.1-10 nM E2. Neosynthesised proteoglycans (PGs) were evaluated after incorporation of [(35)SO(4)]sulphate and further analysed after chromatography on a Sepharose 2B column. Chondrocyte mRNA levels of aggrecan, MMP-1, -3, -13, and tissue inhibitor of metalloproteinase-1 (TIMP-1) were studied by northern blot. MMP-1 activity was measured by zymography. MMP-1 gene transcription was studied by transient transfection of chondrocytes with an MMP-1-luciferase construct. RESULTS: E2 modulated the IL1beta-induced total sulphated PGs in rabbit articular chondrocytes, which decreased as the E2 concentration was increased. At a low concentration (0.1 nmol/l) E2 counteracts the IL1beta-induced decrease in sulphated PG, while at high concentration (10 nmol/l) E2 enhances the IL1beta effects. A biphasic E2 effect was also observed on IL1beta-induced disaggregation of PG, 53-58 kDa gelatinolytic activity, and MMP-1, -3, and -13 mRNA levels. In contrast, E2 did not modify the level of aggrecan mRNA and had no effect on TIMP-1 mRNA expression. Finally, simultaneous addition of IL1beta and E2 (0.1-10 nmol/l) did not modify IL1beta-induced MMP-1-luciferase activity, suggesting that E2 effects probably occur at the post-transcriptional level of MMP gene expression. CONCLUSION: Oestrogen concentration may have an inverse effect on IL1beta stimulated proteoglycan degradation and MMP production by chondrocytes.
Assuntos
Condrócitos/efeitos dos fármacos , Estradiol/farmacologia , Proteínas da Matriz Extracelular , Interleucina-1/farmacologia , Proteoglicanas/metabolismo , Agrecanas , Animais , Condrócitos/enzimologia , Condrócitos/metabolismo , Colagenases/genética , Depressão Química , Expressão Gênica , Lectinas Tipo C , Metaloproteinase 1 da Matriz/genética , Metaloproteinase 13 da Matriz , Metaloproteinase 3 da Matriz/genética , Osteoartrite/metabolismo , Proteoglicanas/genética , RNA Mensageiro/análise , Coelhos , Inibidor Tecidual de Metaloproteinase-1/genéticaRESUMO
STUDY DESIGN: Anulus fibrosus cells from rabbits were grown in primary culture 1) to study their ability to produce prostaglandin E2 and Type II phospholipase A2, and to express stromelysin-1 messenger ribonucleic acid; and 2) to study the effect of interleukin 1 beta on this production and on proteoglycan aggregation. OBJECTIVES: To investigate the potency of anulus fibrosus cells to respond to interleukin 1 beta by producing degradative and inflammatory agents as compared with the potency of articular chondrocytes in the same animal. SUMMARY OF BACKGROUND DATA: Interleukin 1 beta has been implicated in the degradation of intervertebral discs. The way anulus fibrosus cells differ from articular chondrocytes in their responses to interleukin 1 beta remains to be established. METHODS: Anulus fibrosus cells and articular chondrocytes were obtained from young rabbits, grown in primary culture, and incubated with interleukin 1 beta. The newly synthesized proteoglycan was measured by labeling with [35S]-sulfate. Proteoglycan aggregation was analyzed by the elution profile on Sepharose 2B columns. The contents of collagen Type II and stromelysin-1 messenger ribonucleic acid were assessed by Northern blot analysis. The Type II phospholipase A2 activity was measured using a fluorometric substrate. Prostaglandin E2 production was evaluated by radioimmunoassay. RESULTS: Anulus fibrosus cells had 2.5-fold less Type II collagen messenger ribonucleic acid than articular chondrocytes, and interleukin 1 beta had no significant effect on this. Anulus fibrosus cells synthesized and secreted four-fold less proteoglycan than articular chondrocytes. Interleukin 1 beta reduced the anulus fibrosus content of total [35S]-sulfated proteoglycan by 35% (P < 0.01), and that of articular cells by 41% and decreased proteoglycan aggregation. Interleukin 1 beta induced the production of stromelysin-1 messenger ribonucleic acid in both cell types. The stromelysin-1 messenger ribonucleic acid content of anulus fibrosus cells was one half that of articular cells. Interleukin 1 beta increased the production of prostaglandin E2 and caused a dose-dependent secretion of Type II phospholipase A2 activity in both cell types. Its effect was 2.5-fold lower in anulus fibrosus cells than in articular chondrocytes. CONCLUSION: Anulus fibrosus cells can be stimulated by interleukin 1 beta to produce factors implicated in local degradative and inflammatory processes. This production is associated with decreased proteoglycan aggregation. Anulus fibrosus cells respond slightly less well to interleukin 1 beta in vitro than do articular cells.
Assuntos
Cartilagem Articular/citologia , Cartilagem Articular/metabolismo , Condrócitos/efeitos dos fármacos , Interleucina-1/farmacologia , Disco Intervertebral/citologia , Disco Intervertebral/metabolismo , Animais , Northern Blotting , Células Cultivadas , DNA/análise , Dinoprostona/biossíntese , Fosfolipases A/biossíntese , Fosfolipases A2 , Proteoglicanas/biossíntese , RNA/análise , CoelhosRESUMO
This prospective longitudinal study was undertaken to examine the short-term effects (6 months) of estrogen withdrawal on the circulating IGF system. A series of 40 patients suffering from endometriosis was studied before and after a 6-month treatment period with gonadotrophin releasing hormone (GnRH) agonist and calcium, with or without nasal salmon calcitonin. The plasma concentrations of insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF-II) were measured by radioimmunoassay and radioreceptor assay respectively. Plasma IGF binding proteins (IGFBPs) were quantified and characterized by ligand blot and immunoblot. In all patients, a secondary hypoestrogenism was observed, including a 4% decrease in lumbar bone mineral density (L-BMD). The plasma IGF-I and IGF-II concentrations increased after treatment (24%, p < 0.0005 and 40%, p < 0.004 respectively), with no significant difference between the treatment groups. There was a positive correlation between plasma IGF-I (but not IGF-II) changes and changes in urinary deoxypyridinoline (r = 0.32, p < 0.05), urinary C telopeptide of type 1 collagen (r = 0.33, p < 0.04) and total plasma alkaline phosphatases (r = 0.33, p < 0.04). No correlation was found between IGF-I and L-BMD changes, while there was a positive correlation between the changes in plasma IGF-II and L-BMD (r = 0.32, p < 0.05). Ligand blot analysis revealed a significant increase in IGF-II binding to a 29-31 kilodalton region where positive staining with specific antibodies to IGFBP-3 or IGFBP-1 was observed. In conclusion, IGF-I and IGF-II plasma concentrations are both increased following a short period of treatment with a GnRH agonist. The changes in individual IGF peptides are differently correlated with changes in markers of bone remodelling and L-BMD.
Assuntos
Hormônio Liberador de Gonadotropina/agonistas , Fator de Crescimento Insulin-Like II/metabolismo , Fator de Crescimento Insulin-Like I/metabolismo , Luteolíticos/farmacologia , Pamoato de Triptorrelina/farmacologia , Doença Aguda , Adulto , Western Blotting , Densidade Óssea/efeitos dos fármacos , Osso e Ossos/metabolismo , Eletroforese em Gel de Poliacrilamida , Estrogênios/deficiência , Feminino , Humanos , Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/sangue , Fator de Crescimento Insulin-Like I/efeitos dos fármacos , Fator de Crescimento Insulin-Like II/efeitos dos fármacos , Estudos ProspectivosRESUMO
The IR-IGF1 production by rabbit epiphyseal chondrocytes cultured in serum-free medium was analyzed. Cell proliferation was induced by the addition of 10 ng/ml basic fibroblast growth factor (bFGF) without or with 100 ng/ml recombinant human growth hormone (hGH). GH alone induced no cell multiplication. Chondrocytes treated with bFGF alone secreted an IR-IGF1 activity proportional to the mitotic activity of the cells. A specific positive IGF1 immunostaining was localized in the Golgi of control and hGH-treated cells. The IR-IGF1 activity recovered into culture medium was mainly composed of three fractions of apparent MW 6-8 kDa, 9-14 kDa, and 16-18 kDa. [35S]Methionine pulse-chase experiments indicated that the radiolabeled 16-18 kDa IR-IGF1 fraction was partly converted into the 9-14 kDa and 6-8 kDa fractions. At equilibrium, 70% of the chondrocyte IR-IGF1 activity was recovered as 9- to 18-kDa forms which contained high IR-proIGF1A activity. The 6-8 kDa fraction had biochemical characteristics similar to those of the mature IGF1 peptide. Similar results were observed when 4% fetal calf serum was added to the culture. The addition of 100 ng/ml of hGH significantly and specifically increased IGF1 precursor material, which thus represented 90% of total IR-IGF1 activity. On Day 16 of the culture, when cells stopped dividing, the amount of chondrocyte IR-IGF1 was significantly lower than during cell proliferation, and hGH had no effect on this production. These data indicate that cultured chondrocytes produce more IGF1 precursors than mature IGF1 and that GH specifically stimulates biosynthesis of IGF1 precursors but not IGF1 per se. A GH-dependent biological function of IGF1 proforms in chondrocytes remains to be demonstrated.
Assuntos
Cartilagem Articular/metabolismo , Fator 2 de Crescimento de Fibroblastos/fisiologia , Hormônio do Crescimento/fisiologia , Fator de Crescimento Insulin-Like I/biossíntese , Animais , Células Cultivadas , Cromatografia Líquida de Alta Pressão , Meios de Cultura Livres de Soro , Humanos , Concentração de Íons de Hidrogênio , Imuno-Histoquímica , Fator de Crescimento Insulin-Like I/fisiologia , Focalização Isoelétrica , Microscopia Eletrônica , Testes de Precipitina , CoelhosRESUMO
Prepubertal rabbit epiphyseal chondrocytes were grown in high density primary culture for 3 d. They were then incubated for 3 additional d in serum-free culture medium to which bFGF (1-50 ng/ml) was added. During the last 24 h incubation period, either IGF1 (1-80 ng/ml) or Insulin (1-5 micrograms/ml) was added to the culture medium. Chondrocyte DNA was significantly augmented with the increasing concentration of bFGF used, thus confirming its mitogenic effect on chondrocytes. On the other hand, bFGF was also shown to modulate the phenotypic expression of the chondrocytes. The 35S-sulfate incorporation into newly synthesized proteoglycans by the cultured cells decreased in a dose-dependent manner with bFGF concentration used. In addition, chondrocyte collagen gene expression was also shown to be modulated by bFGF. Total RNA extracted from the cultured cells was analyzed by dot blot and Northern blot with cDNA probes encoding for alpha 1 II and alpha 1 I procollagen chains. A significant lower level of type II collagen mRNA, the marker of chondrocytic phenotype, was observed when cells were grown in the presence of bFGF while the level of type I mRNA remained unchanged. When IGF1 or a high concentration of insulin was added to the cells during the last 24 h of incubation with bFGF, sulfated proteoglycan synthesis, as well as collagen type II mRNA level, were significantly stimulated when compared with chondrocytes incubated with bFGF alone. In conclusion, in the present experimental conditions, bFGF appears to be a growth promoting agent for chondrocytes in vitro with dedifferentiating action on chondrocyte phenotype. IGF1 or insulin used at a high concentration can prevent the dedifferentiating effect of bFGF without inhibiting its stimulating effect on chondrocyte DNA synthesis.
Assuntos
Colágeno/genética , Fatores de Crescimento de Fibroblastos/farmacologia , Expressão Gênica , Lâmina de Crescimento/metabolismo , Fator de Crescimento Insulin-Like I/farmacologia , Proteoglicanas/biossíntese , Somatomedinas/farmacologia , Sulfatos/metabolismo , Animais , Diferenciação Celular , Divisão Celular , Células Cultivadas , Sondas de DNA , Lâmina de Crescimento/efeitos dos fármacos , Insulina/farmacologia , Hibridização de Ácido Nucleico , Fenótipo , RNA Mensageiro/metabolismo , CoelhosRESUMO
This paper reports data on the in vitro effects of insulin-like growth factor I (IGF-I) and basic fibroblast growth factor (bFGF) on the phenotypic expression of epiphyseal chondrocytes grown in serum-free (SF) culture medium. bFGF mostly stimulates chondrocyte DNA and inhibits sulfated proteoglycan synthesis and type II collagen mRNA. On the contrary, IGF-I is poorly mitogenic but strongly stimulates protein synthesis and type II collagen mRNA. In addition, IGF-I prevents the expression of type I collagen gene. Lastly, chondrocytes cultured in SF medium are able to locally produce IGF-I peptides. In conclusion, IGF-I and bFGF have opposite effects on the phenotypic expression of chondrocytes in vitro: bFGF is mostly mitogenic and IGF-I appears to be a differentiating factor.
Assuntos
Fator 2 de Crescimento de Fibroblastos/farmacologia , Lâmina de Crescimento/metabolismo , Fator de Crescimento Insulin-Like I/farmacologia , Animais , Colágeno/genética , DNA/biossíntese , Expressão Gênica , Fator de Crescimento Insulin-Like I/biossíntese , Fenótipo , Proteoglicanas/metabolismo , RNA Mensageiro/biossíntese , Sulfatos/metabolismoRESUMO
To determine whether growth hormone has a direct effect on skeletal tissues not mediated by somatomedins, and to better define the role of somatomedin-C/insulin-like growth factor I (Sm-C/IGF-I) in skeletal development, bovine growth plate, and rabbit articular and growth plate chondrocytes in primary culture were evaluated under a variety of experimental conditions designed to elicit growth hormone and Sm-C/IGF-I stimulation. Under none of these conditions did bovine growth plate chondrocytes respond to either homologous bovine growth hormone or heterologous hGH. Under the same conditions, these cells were highly responsive to human Sm-C/IGF-I with respect to both [3H]thymidine and [35S]sulfate incorporation, indices of mitotic and differentiated cell functions, respectively. Similarly, both rabbit articular and growth plate chondrocytes showed enhanced incorporation of [3H] thymidine and [35S]sulfate in the presence of Sm-C/IGF-I, but did not respond to either native or recombinant hGH. Cells at different stages of maturation within the bovine growth plate differed in their reaction to Sm-C/IGF-I with proliferative zone cells manifesting a greater response to the peptide than cells of the reserve zone. These results suggest that the action of Sm-C/IGF-I on growth plate and articular chondrocytes is direct and that the effect of GH on these cells is indirect. The data further suggest that within the growth plate, the transition from reserve to proliferative status is associated with an increased Sm-C/IGF-I responsiveness, a change which may contribute to the functional differences in these cells.
Assuntos
Cartilagem Articular/efeitos dos fármacos , Hormônio do Crescimento/farmacologia , Lâmina de Crescimento/efeitos dos fármacos , Fator de Crescimento Insulin-Like I/farmacologia , Somatomedinas/farmacologia , Animais , Cartilagem Articular/citologia , Bovinos , Células Cultivadas , Relação Dose-Resposta a Droga , Lâmina de Crescimento/citologia , CoelhosRESUMO
This study concerns the immunoreactive somatomedin C secretion by prepubertal rabbit epiphyseal chondrocytes cultured in a defined serum-free medium. In such culture conditions, chondrocytes mainly synthesized Type II collagen (80% of total collagen) during 10 days. A small amount of Type I collagen was also found with a significant (p less than 0.05) higher level during the period of cell multiplication (6.4 +/- 1.5%) than when cells reached confluency (0.9 +/- 0.2%). During the 10 days of culture without serum and without hormone added, a Sm-C/IGF1 activity was measured by RIA at a mean level of 30 +/- 5 mU/ml/10 micrograms DNA. This value was significantly higher (p less than 0.001) than in the medium not incubated with the cells (1.7 +/- 0.9 mU/ml). When hGH was added to the culture medium during the period of cell division, the level of Sm-C/IGF1 activity was significantly elevated at 39 +/- 4 mU/ml/10 micrograms DNA (p less than 0.05) and at 55 +/- 3 mU/ml/10 micrograms DNA (p less than 0.001) with 50 ng/ml and 100 ng/ml hGH concentrations respectively. On the contrary, no difference was observed at confluency in treated and non treated cells.
Assuntos
Hormônio do Crescimento/farmacologia , Lâmina de Crescimento/citologia , Fator de Crescimento Insulin-Like I/metabolismo , Somatomedinas/metabolismo , Animais , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Colágeno/biossíntese , Lâmina de Crescimento/efeitos dos fármacos , Lâmina de Crescimento/metabolismo , Masculino , Coelhos , Maturidade SexualRESUMO
Proteoglycans synthesized by articular and epiphyseal chondrocytes in culture were compared. Proteoglycans extruded by the two types of cells into the culture medium are of identical Mr. On the other hand, the proteoglycans of cells or pericellular matrix synthesized by the articular chondrocytes are characterized by an heterogeneous fraction of low-Mr which is not present in the material derived from epiphyseal chondrocytes. There are at least two components in this fraction: the first seems to be a precursor of aggregated proteoglycans, the other may represent a component of cell coat. Stimulation of the cell cultures with vitamin D metabolites and somatomedin enhances proteoglycan biosynthesis but no modification is observed in the proteoglycan Mr.
Assuntos
Cartilagem Articular/metabolismo , Proteoglicanas/biossíntese , Animais , Cartilagem Articular/efeitos dos fármacos , Células Cultivadas , Cromatografia em Gel , Epífises/metabolismo , Insulina/farmacologia , Peso Molecular , Peptídeos/farmacologia , Coelhos , Somatomedinas/farmacologia , Sulfatos/metabolismo , Fatores de Tempo , Vitamina D/farmacologiaRESUMO
Interactions between cartilage and vitamin D metabolism were studied in vitro. Chondrocytes were isolated from rabbit growth plate cartilage and cultured. These cells are able to transform 25-hydroxycholecalciferol in 24, 25-dihydroxycholecalciferol. Metabolic transformation is modulated by 1, 25-dihydroxycholecalciferol but not by parathyroid hormone neither by extracellular calcium concentrations. 24, 25-(OH) 2D3 and 1, 25-(OH) 2D3 are active on the cellular metabolism of cultured chondrocytes in a different way : 24, 25-(OH) 2D3 stimulates the proteoglycan synthesis in "mature" chondrocytes 1, 25-(OH) 2D3 increases DNA polymerase activities in chondrocytes during the logarithmic phase of division. Finally, specific sites of nuclear receptors of 24, 25-(OH) 2D3 are present in chondrocytes.
Assuntos
Cartilagem/metabolismo , Vitamina D/metabolismo , Animais , Calcifediol , Calcitriol/metabolismo , Células Cultivadas , Di-Hidroxicolecalciferóis/metabolismo , Hidroxicolecalciferóis/metabolismo , Coelhos , Receptores de Calcitriol , Receptores de Esteroides/metabolismoRESUMO
Chondrocytes from normal cartilage of a 2 month old child have been cultured. In vitro the cells maintained their morphological identity and synthesised proteoglycans with electrophoretic properties similar to those synthesised by cartilage in vivo. Sulphation was stimulated by vitamin D metabolites and by somatomedin. This technique has been used to study biopsies of abnormal cartilage.
Assuntos
Cartilagem Articular/metabolismo , Somatomedinas/farmacologia , Vitamina D/metabolismo , Cartilagem Articular/patologia , Diferenciação Celular , Células Cultivadas , Condroitina/metabolismo , Eletroforese em Gel de Poliacrilamida , Humanos , Lactente , Proteoglicanas/metabolismoRESUMO
Chondrocyte cultures were prepared from rabbit growth plate (GPC) and articular (ARC) chondrocytes. These two cell types have distinct morphological characteristics. The cells reached maximum numbers by days 10 and 21 for ARC and GPC, respectively. The proteoglycans (PG) contained in the cellular pool were extracted and purified by DEAE cellulose chromatography. The effect of a partially purified somatomedin peptide with insulin-like activity on [35S]sulphate incorporation into PG was evaluated. In both ARC and GPC a significant stimulation of [35S]sulphate uptake into PG subunits was obtained with 1 ng Eq./ml of somatomedin peptide. In order to obtain the same stimulatory effect with porcine insulin, a 1000-fold greater concentration was required. The electrophoretic patterns of the PG subunits on acrylamide-agarose electrophoresis were identical on control incubations and after stimulation with the somatomedin peptide. These data demonstrate in vitro biological activity of this peptide on well differentiated articular and epiphyseal growth plate chondrocytes in culture. These cultures appear to provide a sensitive biological assay for somatomedin peptides.
Assuntos
Cartilagem Articular/metabolismo , Cartilagem/metabolismo , Peptídeos/farmacologia , Proteoglicanas/metabolismo , Somatomedinas/farmacologia , Sulfatos/metabolismo , Animais , Cartilagem/citologia , Cartilagem Articular/citologia , Células Cultivadas , Meios de Cultura , DNA/metabolismo , Ácidos Hexurônicos/metabolismo , Humanos , Proteoglicanas/biossíntese , Proteoglicanas/isolamento & purificação , CoelhosRESUMO
Chondrocytes from epihyseal growth plate cartilage of prepubertal rabbits were cultured. Cells from the proliferative zone showed a very different adhesiveness: they did not stick to the flasks until the 8th to 10th days. They need more oxygen tension to develop and grew in small separated colonies. Each colony was composed by several layers of round or polygonal cells, metachromatic with toluidine blue and they were surrounded by one layer of fibroblastic-like cells. At the end of primary culture the colonies looked like "craters". Cells from the resting zone multiplied as monolayer culture identical to articular chondrocytes. The stage of differentiation of the two different kinds of cells is discussed.