Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
Tissue Eng Part C Methods ; 30(8): 343-352, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39078332

RESUMO

Ex vivo 3D culture of human tissue explants addresses many limitations of traditional monolayer cell culture techniques, namely the lack of cellular heterogeneity and absence of 3D intercellular spatial relationships, but presents challenges with regard to repeatability owing to the difficulty of acquiring multiple tissue samples from the same donor. In this study, we used a cryopreserved bank of human lung microexplants, ∼1 mm3 fragments of peripheral lung from donors undergoing lung resection surgery, and a liquid-like solid 3D culture matrix to describe a method for the analysis of non-small-cell lung cancer adhesion to human lung tissue. H226 (squamous cell carcinoma), H441 (lung adenocarcinoma), and H460 (large cell carcinoma) cell lines were cocultured with lung microexplants. Confocal fluorescence microscopy was used to visualize the adherence of each cell line to lung microexplants. Adherent cancer cells were quantified following filtration of nonadherent cells, digestion of cultured microexplants, and flow cytometry. This method was used to evaluate the role of integrins in cancer cell adherence. A statistically significant decrease in the adherence of H460 cells to lung microexplants was observed when anti-integrins were administered to H460 cells before coculture with lung microexplants.


Assuntos
Adesão Celular , Neoplasias Pulmonares , Pulmão , Humanos , Neoplasias Pulmonares/patologia , Linhagem Celular Tumoral , Pulmão/patologia , Pulmão/citologia , Técnicas de Cultura de Células em Três Dimensões/métodos , Técnicas de Cocultura/métodos , Carcinoma Pulmonar de Células não Pequenas/patologia , Integrinas/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA