RESUMO
The paper presents the results of isolation of influenza A virus from 97 cloacal swabs of 11 species of aquatic and semiaquatic wild birds collected on the Chany Lake (the south of Western Siberia, Ob-Irtysh interarea). Six strains with subtypes H2 (2 strains), H3 (3 strains), and H5 (1 strain) were isolated from mallard ducks (Anas platyrhynchos). The total infection rate in the examined birds was 6.2% and that in the ducks was 9.7%. The paper deals with the phylogenetic analysis of hemagglutinin of genes of isolates and with the comparison of the obtained results with the 2002 data in the same region. Analysis of H5 strain hemagglutinin proteolytic site permits one to regard this strain as non-pathogenic.
Assuntos
Aves/virologia , Monitoramento Ambiental , Vírus da Influenza A/isolamento & purificação , Influenza Aviária/prevenção & controle , Animais , Antígenos Virais/classificação , Antígenos Virais/genética , Embrião de Galinha , Cloaca/virologia , Glicoproteínas de Hemaglutininação de Vírus da Influenza/classificação , Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética , Vírus da Influenza A/classificação , Vírus da Influenza A/genética , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , SibériaRESUMO
Influenza A virus variants belonging to H3 and H4 subtypes were isolated from wild ducks inhabiting in the south of Western Siberia. Phylogenetic analysis of hemagglutinin (HA) gene of these viruses has revealed that H3 isolates are closely related to those isolated from the bird inhabiting in West Europe (A/Teal/Germany/wv01r/01, A/Duck/Ukraine/1/63) and China (A/Aquatic bird/Hong Kong/399/99); and those isolated from the birds inhabiting in Germany (A/Garganey/Germany/wv157k/01, A/Teal/Germany/wv153k/01). Thus, closely related influenza A virus variants circulate in the populations of the wild birds inhabiting in greatly spaced regions of Eurasia.
Assuntos
Aves/virologia , Variação Genética , Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética , Vírus da Influenza A/genética , Filogenia , Animais , Vírus da Influenza A/isolamento & purificação , Ratos , SibériaRESUMO
As the result of the study of Caspian seals, the presence of antibodies to Brucella was detected in the blood serum of these animals. The annual dynamics of the proportion of seropositive animals was noted. These data indicate the possibility of the circulation of the infective agent of brucellosis in the population of Caspian seals.
Assuntos
Anticorpos Antibacterianos/sangue , Brucella/imunologia , Água Doce , Focas Verdadeiras/sangue , Animais , Brucelose/epidemiologia , Brucelose/veterinária , Federação Russa/epidemiologia , Estações do Ano , Estudos SoroepidemiológicosRESUMO
The nucleotide sequences were determined for a phosphoprotein gene fragment of canine distemper virus (CDV) by using the RT-PCR method with the subsequent sequencing of amplicons from total RNA isolated from 2 samples of Caspian seals, 15 samples of Baikal seals and from samples of dog's and sea-lion's brains. The above materials were phylogenetically analyzed. The heterogeneity of the virus circulating in the Baikal-seal population was demonstrated. Morbillivirus, that caused epizooty in Caspian seals, was shown to be a CDV variant, whose phosphoprotein gene structure was not different, within the analyzed stretch, from the corresponding gene of the most widespread variant of the Baikal seal virus. The data obtained suggest that morbillivirus could be transmitted by birds during their seasonal migrations.