1.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 67(Pt 1): 72-5, 2011 Jan 01.
Artigo
em Inglês
| MEDLINE
| ID: mdl-21206028
RESUMO
The genome of the enteric pathogen Campylobacter jejuni encodes a single glyceraldehyde-3-phosphate dehydrogenase that can utilize either NADP+ or NAD+ as coenzymes for the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate. Here, the cloning, expression, purification, crystallization and preliminary X-ray analysis of both the wild type and an active-site mutant of the enzyme are presented. Preliminary X-ray analysis revealed that in both cases the crystals diffracted to beyond 1.9â Å resolution. The space group is shown to be I4(1)22, with unit-cell parameters a=90.75, b=90.75, c=225.48â Å, α=90.46, ß=90.46, γ=222.79°; each asymmetric unit contains only one subunit of the tetrameric enzyme.