Molecular Diversity and Combining Ability in Newly Developed Maize Inbred Lines under Low-Nitrogen Conditions.

Nitrogen is an essential element for maize growth, but excessive application can lead to various environmental and ecological issues, including water pollution, air pollution, greenhouse gas emissions, and biodiversity loss. Hence, developing maize hybrids resilient to low-N conditions is vital for sustainable agriculture, particularly in nitrogen-deficient soils. Combining ability and genetic relationships among parental lines is crucial for breeding superior hybrids under diverse nitrogen levels. This study aimed to assess the genetic diversity of maize inbred lines using simple sequence repeat (SSR) markers and evaluate their combining ability to identify superior hybrids under low-N and recommended conditions. Local and exotic inbred lines were genotyped using SSR markers, revealing substantial genetic variation with high gene diversity (He = 0.60), moderate polymorphism information content (PIC = 0.54), and an average of 3.64 alleles per locus. Twenty-one F1 hybrids were generated through a diallel mating design using these diverse lines. These hybrids and a high yielding commercial check (SC-131) were field-tested under low-N and recommended N conditions. Significant variations (p < 0.01) were observed among nitrogen levels, hybrids, and their interaction for all recorded traits. Additive genetic variances predominated over non-additive genetic variances for grain yield and most traits. Inbred IL3 emerged as an effective combiner for developing early maturing genotypes with lower ear placement. Additionally, inbreds IL1, IL2, and IL3 showed promise as superior combiners for enhancing grain yield and related traits under both low-N and recommended conditions. Notably, hybrids IL1×IL4, IL2×IL5, IL2×IL6, and IL5×IL7 exhibited specific combining abilities for increasing grain yield and associated traits under low-N stress conditions. Furthermore, strong positive associations were identified between grain yield and specific traits like plant height, ear length, number of rows per ear, and number of kernels per row. Due to their straightforward measurability, these relationships underscore the potential of using these traits as proxies for indirect selection in early breeding generations, particularly under low-N stress. This research contributes to breeding nitrogen-efficient maize hybrids and advances our understanding of the genetic foundations for tolerance to nitrogen limitations.

Interaction Effects of Nitrogen Source and Irrigation Regime on Tuber Quality, Yield, and Water Use Efficiency of Solanum tuberosum L.

Two field experiments were conducted to investigate the effects of three drip irrigation regimes (G1: 120% crop evapotranspiration (ETc), G2: 100% ETc, and G3: 80% ETc) and four nitrogen (N) source treatments (S0: non-fertilized; S1: urea, S2: ammonium nitrate, and S3: ammonium sulfate on water consumption use, water utilization efficiency, chlorophyll, yield and tubers quality of potato (Solanum tuberosum L.; cv Diamond) under a drip irrigation system during two successive winter seasons (2015/16 and 2016/17)). Nitrogen fertilization was applied at 380 kg ha-1 as standard application for potato in the investigated area. The highest tubers yield was obtained from potato grown with G1 S2 (65.8 Mg ha-1), G1 S3 (63.6 Mg ha-1), G2 S2 (64.1 Mg ha-1), and G2 S3 (62.4 Mg ha-1), while the lowest tubers yield was obtained from potato grown with G3 S0 (10.1 Mg ha-1) and G2S0 (17.4 Mg ha-1). Different treatments of N source resulted in a significant increase for water use efficiency (WUtE) compared with unfertilized treatment. For the interaction effect, the highest WUtE was obtained from potato grown with G3 S2 (18.1 kg m-3), followed by G3 S3 (17.6 kg m-3), while the lowest WUtE was obtained from plants grown with G3S0 (3.0 kg m-3). However, the highest chlorophyll content was obtained from plants grown with G1 and any N source, followed by G2S1-3, while the lowest chlorophyll content was obtained from those grown with G3S0. The highest N, S, protein, and P contents in tubers were obtained from plants grown with G3S3, G3S2, and G2S2, while the highest K content in tubers was obtained from plants grown with G1S1 and G1S2. In concussion, the integrative effects of G1 or G2 with S2 or S3 is recommended for high productivity, while the integrative effects of G3S3 and G3S2 are recommended for high quality tubers.

Screening for developmental dysplasia of the hip in Hong Kong.

PURPOSE: To review records of children referred to our hospital for developmental dysplasia of the hip (DDH) and evaluate the effectiveness of hip screening in Hong Kong. METHODS: Records of children suspected of DDH and referred to our hospital between 1 January 2005 and 31 March 2010 were reviewed. The diagnosis was based on static and dynamic ultrasonography of the hips and/or radiographic features of hip subluxation, dislocation, and pelvic dysplasia. The age of the children at presentation was recorded. Late diagnoses were those presenting after the age of 6 months. Late presentations were those presenting after the walking age. RESULTS: Of 213 referred children, 33 were diagnosed to have DDH. Their mean age at presentation was 90.8 (range, 9-559) days. The female-to-male ratio was 2.9:1, and the mean follow-up duration was 25.1 (range, 4.2-50.3) months. 30 of the DDH infants were diagnosed before the age of 6 months, one at 8 months, and 2 were late presenters. Hence, the failure rate of the hip screening was 0.09 per 1000 live births. The remaining 180 were confirmed to be normal and followed up until the walking age. The point prevalence of DDH on Hong Kong Island was 0.87/1000 live births. CONCLUSION: In light of the low point prevalence of DDH in Hong Kong born children, the protocol of universal clinical assessment and selective ultrasonographic screening of suspected cases is justified.

Minimally invasive, no hardware subtalar arthrodesis with autogenous posterior iliac bone graft.

Sixteen patients underwent minimally invasive subtalar arthrodesis through a mini-invasive approach with posterior iliac graft between 2004 and 2006. No hardware was used to transfix the arthrodesis and partial weight bearing was allowed immediately. The primary indication for surgery was the squeal of fracture os calcis in terms of subtalar joint arthritis, loss of heel height, malalignment of the hindfoot, and pain with weight bearing. There were 12 male and 4 female patients with a mean age of 30 (range 17-52). Patients were followed up for a period of 40.8 months (range 36-48 months). The mean interval from injury to fusion was 2 (+0.6) years ranging from 6 months to 6 years post fracture. The average clinical rating scale based on the American Orthopaedic Foot and Ankle Society (AOFAS) improved from 36 preoperatively to 78 at the latest follow-up (P < 0.05). Union rate was 94%. Radiographic evaluation revealed a mean increase in calcaneal inclination of 6.25 + 8.3 degrees (P < 0.07) and a mean increase in the lateral talocalcaneal angle of 7.42 + 10.2 degrees (P < 0.08). Complications were graft nonunion in 1 patient and transient tendoachilles tendinitis in another. This technique can be used to decrease the morbidity associated with the late complications of os calcis fractures by aligning the hindfoot, restoring the heel height and correcting calcaneal and talar inclination. It offers the advantage of early weight bearing while avoiding hardware complications.