Micro-CT observation of in vivo temporal change in mandibular condyle morphology in BMAL1 knockout mice.

Brain and muscle Arnt-like protein-1 (BMAL1) knockout mice exhibit accelerated aging, abnormal glucose metabolism, and impaired adipocyte differentiation, among other phenotypes, which are effects associated with the BMAL1 gene. No study has investigated temporal changes in the deformation of the mandibular condyle and the presence of calcification in areas surrounding the mandibular condyle. In a study of 12 C57/BL strain mice under inhalation anesthesia, we collected images of the mandibular condyle at 6 weeks after birth and then every 5 weeks from 10 to 25 weeks after birth. At 25 weeks, deformation of the mandibular condyle was seen in 8 of 12 joints in BMAL1 knockout mice and in 2 of 12 joints in wild-type mice. At 20 and 25 weeks, deformation in areas surrounding the mandibular condyle, which are known to undergo calcification, was seen in 2 of 12 joints in BMAL1 knockout mice and in 0 of 12 joints in wild-type mice. BMAL1 knockout mice exhibited premature aging of the mandibular condyle, which suggests that circadian rhythms affect mandibular condyle morphology.

Influence of lead apron shielding on absorbed doses from cone-beam computed tomography.

The aim of the present work was to investigate absorbed and to calculate effective doses (EDs) in cone-beam computed tomography (CBCT). The study was conducted using examination protocols with and without lead apron shielding. A full-body male RANDO® phantom was loaded with 110 GR200A thermoluminescence dosemeter chips at 55 different sites and set up in two different CBCT systems (CS 9500®, ProMax® 3D). Two different protocols were performed: the phantom was set up (1) with and (2) without a lead apron. No statistically significant differences in organ and absorbed doses from regions outside the primary beam could be found when comparing results from exposures with and without lead apron shielding. Consequently, calculating the ED showed no significant differences between the examination protocols with and without lead apron shielding. For the ProMax® 3D with shielding, the ED was 149 µSv, and for the examination protocol without shielding 148 µSv (SD = 0.31 µSv). For the CS 9500®, the ED was 88 and 86 µSv (SD = 0.95 µSv), respectively, with and without lead apron shielding. The results revealed no statistically significant differences in the absorbed doses between examination with and without lead apron shielding, especially in organs outside the primary beam.

Are assessments of damping capacity and placement torque useful in estimating root proximity of orthodontic anchor screws?

INTRODUCTION: Placement torque and damping capacity may increase when the orthodontic anchor screws make contact with an adjacent root. If this is the case, root contact can be inferred from the placement torque and damping capacity. The purpose of this study was to verify the detectability of root proximity of the screws by placement torque and damping capacity. For this purpose, we investigated the relationship among placement torque, damping capacity, and screw-root proximity. METHODS: The placement torque, damping capacity, and root proximity of 202 screws (diameter, 1.6 mm; length, 8.0 mm) were evaluated in 110 patients (31 male, 79 female; mean age, 21.3 ± 6.9 years). Placement torque was measured using a digital torque tester, damping capacity was measured with a Periotest device (Medizintechnik Gulden, Modautal, Germany), and root contact was judged using cone-beam computed tomography images. RESULTS: The rate of root contact was 18.3%. Placement torque and damping capacity were 7.8 N·cm and 3.8, respectively. The placement torque of screws with root contact was greater than that of screws with no root contact (P <0.05; effect size, 0.44; power, <0.8). Damping capacity of screws with root contact was significantly greater than that of screws with no root contact (P <0.01; effect size, >0.5; power, >0.95). CONCLUSIONS: It was suggested that the damping capacity is related to root contact.

Assessment of quality and interpretation of panoramic radiographs obtained in the Lao People's Democratic Republic as part of a teleradiology collaboration with Japan.

As part of quality assessment of a teleradiology program we evaluated the validity of patient information received, the quality of panoramic radiography imaging in Laos, and the ability of a Laotian radiologist to detect temporomandibular joint abnormalities. The amount of patient information gathered from 2,021 scans of panoramic radiographs was evaluated by triage before image diagnosis. Among the radiographs from 2,021 patients, primary triage indicated that there was insufficient information for 794 (39.3%) patients. Secondary triage to assess imaging failure included 1,227 radiographs, four of which were excluded from imaging diagnosis because of unacceptable image flaws. In total, 2,446 joints from 1,223 radiographs were evaluated for temporomandibular joint abnormalities in order to compare the image interpretation abilities of Laotian and Japanese radiologists. The kappa coefficient was 0.836 (P < 0.01) for the agreement between the two observers in detecting temporomandibular joint abnormalities on radiographs. We conclude that additional efforts are needed in order to overcome the challenges of maintaining quality in imaging techniques and diagnoses in Laos.

Locomotion pattern and trunk musculoskeletal architecture among Urodela.

We comparatively examined the trunk musculature and prezygapophyseal angle of mid-trunk vertebra in eight urodele species with different locomotive modes (aquatic Siren intermedia, Amphiuma tridactylum, Necturus maculosus and Andrias japonicus; semi-aquatic Cynops pyrrhogaster, Cynops ensicauda; and terrestrial Hynobius nigrescens, Hynobius lichenatus and Ambystoma tigrinum). We found that the more terrestrial species were characterized by larger dorsal and abdominal muscle weight ratios compared with those of the more aquatic species, whereas muscle ratios of the lateral hypaxial musculature were larger in the more aquatic species. The lateral hypaxial muscles were thicker in the more aquatic species, whereas the M. rectus abdominis was more differentiated in the more terrestrial species. Our results suggest that larger lateral hypaxial muscles function for lateral bending during underwater locomotion in aquatic species. Larger dorsalis and abdominal muscles facilitate resistance against sagittal extension of the trunk, stabilization and support of the ventral contour line against gravity in terrestrial species. The more aquatic species possessed a more horizontal prezygapophyseal angle for more flexible lateral locomotion. In contrast, the more terrestrial species have an increasingly vertical prezygapophyseal angle to provide stronger column support against gravity. Thus, we conclude trunk structure in urodeles differs clearly according to their locomotive modes.

Measuring bone density at orthodontic miniscrew implantation sites using microcomputed tomography.

PURPOSE: This study was performed to determine the accuracy of measurements of bone mineral density (BMD) and cortical bone thickness (CBT) at miniscrew implantation sites in small animals and to verify the usefulness of in vivo microcomputed tomography (micro-CT). MATERIALS AND METHODS: Rat femurs were scanned before and after placing miniscrews using in vivo micro-CT. The images were superimposed using a subtraction method with bone volume measurement software. At each screw site, the total BMD was calculated as the average BMD of a cylinder 1.6 mm in diameter and 2.0 mm in depth, while the cortical BMD was the average BMD of a cylinder 1.6 mm in diameter and 1.0 mm in depth. CBT was measured three times on transaxial images of the rat femurs, and the average value was used. All miniscrews were placed using the maximum torque, verified with a digital torque tester. To verify the usefulness and accuracy of in vivo micro-CT, CBT on micro-CT images was compared with that measured on histologic sections. RESULTS: Significant correlations were observed between placement torque and cortical BMD (R = 0.572), total BMD (R = 0.732), and CBT (R = 0.788). There was a significant correlation between CBT measured via field-emission scanning electron microscopy images and CBT measured with in vivo micro-CT (R = 0.974). CONCLUSIONS: The BMD over a narrow range can be measured accurately in small animals with high reproducibility of the trabecular structure using a combination of high-resolution in vivo micro-CT and image superimposition using a three-dimensional subtraction method.

Influence of metal artifacts on in vivo micro-CT for orthodontic mini-implants.

This study aimed to show the effects of metal artifacts on the in vivo micro-CT of mini-implants by measuring bone volume. We drilled a hole in the cortical bone of a rat tibia and embedded a titanium orthodontic mini-implant (diameter, 1.5 mm) in the hole. Twelve individually weighed hydroxyapatite grains (HA grains) were placed around the implant either by one dentist (method 1) or separately by 12 dentists (method 2). In vivo micro-CT was used to scan the model after placement of each grain to measure increases and decreases in bone volume voxel number. The subtracted bone voxel volume increased with HA weight in both methods. Simple linear regression analysis showed a significant correlation between weight and volume in both methods (method 1: regression coefficient: 516.502, P < 0.05; method 2: regression coefficient: 4837.432, P < 0.05). Metal artifacts did not appear to influence measurements of bone volume, although further studies are required to determine the effect of thicker implants.

Low-level laser therapy enhances the stability of orthodontic mini-implants via bone formation related to BMP-2 expression in a rat model.

OBJECTIVE: The aim of this study was to investigate the stimulatory effects of low-level laser therapy (LLLT) on the stability of mini-implants in rat tibiae. BACKGROUND DATA: In adolescent patients, loosening is a notable complication of mini-implants used to provide anchorage in orthodontic treatments. Previously, the stimulatory effects of LLLT on bone formation were reported; here, it was examined whether LLLT enhanced the stability of mini-implants via peri-implant bone formation. MATERIALS AND METHODS: Seventy-eight titanium mini-implants were placed into both tibiae of 6-week-old male rats. The mini-implants in the right tibia were subjected to LLLT of gallium-aluminium-arsenide laser (830 nm) once a day during 7 days, and the mini-implants in the left tibia served as nonirradiated controls. At 7 and 35 days after implantation, the stability of the mini-implants was investigated using the diagnostic tool (Periotest). New bone volume around the mini-implants was measured on days 3, 5, and 7 by in vivo microfocus CT. The gene expression of bone morphogenetic protein (BMP)-2 in bone around the mini-implants was also analyzed using real-time reverse-transcription polymerase chain reaction assays. The data were statistically analyzed using Student's t test. RESULTS: Periotest values were significantly lower (0.79- to 0.65-fold) and the volume of newly formed bone was significantly higher (1.53-fold) in the LLLT group. LLLT also stimulated significant BMP-2 gene expression in peri-implant bone (1.92-fold). CONCLUSIONS: LLLT enhanced the stability of mini-implants placed in rat tibiae and accelerated peri-implant bone formation by increasing the gene expression of BMP-2 in surrounding cells.

Evaluation of the usefulness of magnetic resonance imaging in the assessment of the thickness of the roof of the glenoid fossa of the temporomandibular joint.

OBJECTIVE: The aim of this study was to evaluate the usefulness of magnetic resonance imaging (MRI) in measuring thickness of the roof of the glenoid fossa (RGF) of the temporomandibular joint (TMJ). STUDY DESIGN: Minimum RGF thickness in 95 TMJs of 59 patients with temporomandibular disorders were measured and compared on both sagittal-section MRI and cone-beam computed tomography (CBCT). RGF thickness on MRI was also compared with MRI, CBCT, and arthrographic findings. RESULTS: Minimum RGF thickness was greater on MRI (1.46 mm) than on CBCT (0.90 mm). Spearman's correlation coefficient by rank for these 2 types of measurements was 0.63. RGF thickness on MRI differed significantly between those with and without degenerative joint changes (1.69 vs 1.32 mm; P < .01) and between those with and without disk displacement (1.58 vs 1.35 mm; P = .04), but showed no associations with disk deformity, joint effusion, or disk perforation. CONCLUSIONS: MRI is useful in measuring RGF thickness from diagnostic as well as radiation protection standpoints.