Estrogen prevents age-dependent beige adipogenesis failure through NAMPT-controlled ER stress pathway.

Thermogenic beige adipocytes are recognized as potential therapeutic targets for combating metabolic diseases. However, the metabolic advantages they offer are compromised with aging. Here, we show that treating mice with estrogen (E2), a hormone that decreases with age, to mice can counteract the aging- related decline in beige adipocyte formation when subjected to cold, while concurrently enhancing energy expenditure and improving glucose tolerance. Mechanistically, we find that nicotinamide phosphoribosyltranferase (NAMPT) plays a pivotal role in facilitating the formation of E2-induced beige adipocytes, which subsequently suppresses the onset of age-related ER stress. Furthermore, we found that targeting NAMPT signaling, either genetically or pharmacologically, can restore the formation of beige adipocytes by increasing the number of perivascular adipocyte progenitor cells. Conversely, the absence of NAMPT signaling prevents this process. In conclusion, our findings shed light on the mechanisms governing the age-dependent impairment of beige adipocyte formation and underscore the E2-NAMPT controlled ER stress as a key regulator of this process. Highlights: Estrogen restores beige adipocyte failure along with improved energy metabolism in old mice.Estrogen enhances the thermogenic gene program by mitigating age-induced ER stress.Estrogen enhances the beige adipogenesis derived from SMA+ APCs.Inhibiting the NAMPT signaling pathway abolishes estrogen-promoted beige adipogenesis.

Licochalcone A plays dual antiviral roles by inhibiting RSV and protecting against host damage.

Respiratory syncytial virus (RSV) causes lower respiratory tract diseases and bronchiolitis in children and elderly individuals. There are no effective drugs currently available to treat RSV infection. In this study, we report that Licochalcone A (LCA) can inhibit RSV replication and mitigate RSV-induced cell damage in vitro, and that LCA exerts a protective effect by reducing the viral titer and inflammation in the lungs of infected mice in vivo. We suggest that the mechanism of action occurs through pathways of antioxidant stress and inflammation. Further mechanistic results demonstrate that LCA can induce nuclear factor erythroid 2-related factor 2 (Nrf2) translocation into the nucleus, activate heme oxygenase 1 (HO-1), and inhibit reactive oxygen species-induced oxidative stress. LCA also works to reverse the decrease in I-kappa-B-alpha (IкBα) levels caused by RSV, which in turn inhibits inflammation through the associated nuclear factor kappa B and tumor necrosis factor-α signaling pathways. The combined action of the two cross-talking pathways protects hosts from RSV-induced damage. To conclude, our study is the first of its kind to establish evidence of LCA as a viable treatment for RSV infection.

Eyelid Blink Lagophthalmos and Blinking Velocity Changes After External Transcutaneous Dacrocystorhinostomy: A Prospective Study.

PURPOSE: To investigate the changes in eyelid blinking dynamics after external dacryocystorhinostomy (DCR). DESIGN: Prospective before-and-after study with follow-up. METHODS: Patients undergoing external DCR surgery for primary acquired nasolacrimal duct obstruction were observed using high-speed videography that recorded eyelid blinking of both eyes for a total duration of 4 minutes at a rate of 120 frames per second. These recordings were taken before the surgical procedure, on postoperative day 3, and then at 1 week, 1 month, and 3 months after the DCR surgery. A total of 3 random blinks were selected and isolated from each video recording for data analysis. For each blink, lagophthalmos and eyelid blinking velocity were calculated for the operated eye, and their values were then compared with those calculated for the contralateral, nonoperated eye. RESULTS: Data were obtained for 22 patients. Operated eyes showed large postoperative lagophthalmos on postoperative day 3, with a mean of 56.58% ± 52.63% of the palpebral fissure height. Gradual improvement of the lagophthalmos occurred over the next follow-up visits, and reached a mean of 31.24% ± 36.71% at follow-up visit 3 months postoperatively. The velocity of eyelid blinking showed a significant reduction for the operated eyes, with gradual improvement over 3 months. CONCLUSIONS: Postexternal DCR eyelid blinking changes included significant blink lagophthalmos with decreased velocity of eyelid blinking which gradually improved over the 3-month follow-up period. A longer follow-up period may be required to confirm if eyelid blinking lagophthalmos and velocity will return to baseline levels.

Adipose stem cells in obesity: challenges and opportunities.

Adipose tissue, the storage of excessive energy in the body, secretes various proteins called adipokines, which connect the body's nutritional status to the regulation of energy balance. Obesity triggers alterations of quantity and quality of various types of cells that reside in adipose tissue, including adipose stem cells (ASCs; referred to as adipose-derived stem/stromal cells in vitro). These alterations in the functionalities and properties of ASCs impair adipose tissue remodeling and adipose tissue function, which induces low-grade systemic inflammation, progressive insulin resistance, and other metabolic disorders. In contrast, the ability of ASCs to recruit new adipocytes when faced with caloric excess leads to healthy adipose tissue expansion, associated with lower amounts of inflammation, fibrosis, and insulin resistance. This review focuses on recent advances in our understanding of the identity of ASCs and their roles in adipose tissue development, homeostasis, expansion, and thermogenesis, and how these roles go awry in obesity. A better understanding of the biology of ASCs and their adipogenesis may lead to novel therapeutic targets for obesity and metabolic disease.