Assuntos
Alelos , Cadeias HLA-DRB1/genética , Haplótipos , Pênfigo/etnologia , Pênfigo/genética , Adulto , Idoso , Árabes , Estudos de Casos e Controles , Egito , Feminino , Expressão Gênica , Frequência do Gene , Alemanha , Cadeias HLA-DRB1/classificação , Cadeias HLA-DRB1/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Pênfigo/imunologia , Pênfigo/patologia , População BrancaRESUMO
BACKGROUND: Umbilical cord blood (UCB) contains stem cells and can be used as an alternative to bone marrow transplantation. Engraftment is dependent on the total nucleated cell (TNC) and CD34+ cell counts of the cord blood units. This study was designed to evaluate the effect of the method of collection of the UCB on the yield of the cord blood units. STUDY DESIGN AND METHODS: Informed consent was obtained from 100 eligible mothers for donation of cord blood. Both in utero and ex utero methods were used for collection. The cord blood volume was measured. The TNC and the CD34+ cell counts were enumerated. RESULTS: We have found that in utero collection gave significantly larger volumes of cord blood and higher TNC counts than ex utero collection. There was no significant difference between both methods regarding the CD34+ cell counts. This study revealed a significant correlation between the volume of the collected cord blood and both TNC and CD34+ cell counts. CONCLUSION: It is better to collect cord blood in utero before placental delivery to optimize the quality of the cord blood unit.
Assuntos
Armazenamento de Sangue/métodos , Doadores de Sangue , Sangue Fetal , Contagem de Células Sanguíneas , Cesárea , Transplante de Células-Tronco de Sangue do Cordão Umbilical , Parto Obstétrico , Egito , Feminino , Células-Tronco Hematopoéticas , Humanos , Recém-Nascido , Gravidez , Fatores de Tempo , Coleta de Tecidos e ÓrgãosRESUMO
This study evaluated peripheral eosinophil and serum eosinophilic cationic protein (s-ECP) levels as markers of asthma control. A total of 38 children with asthma (16 controlled and 22 partially controlled) were compared with 16 age- and sex-matched healthy children. Total asthma cases had higher eosinophil counts and s-ECP levels than healthy children and partially controlled asthmatics had significantly higher levels of both markers than controlled asthmatics. Controlled asthma cases showed non-significant changes in both parameters versus healthy children. A negative correlation was noted between degree of asthma control and both eosinophil counts and s-ECP levels (r = -0.60 and -0.75 respectively). s-ECP as well as peripheral eosinophil count may be helpful in the assessment of asthma control.
Assuntos
Asma/sangue , Proteína Catiônica de Eosinófilo/sangue , Eosinófilos , Contagem de Leucócitos , Albuterol/uso terapêutico , Androstadienos/uso terapêutico , Asma/diagnóstico , Asma/tratamento farmacológico , Asma/imunologia , Biomarcadores/sangue , Broncodilatadores/uso terapêutico , Estudos de Casos e Controles , Criança , Estudos Transversais , Monitoramento de Medicamentos/métodos , Quimioterapia Combinada , Egito , Feminino , Fluticasona , Volume Expiratório Forçado , Humanos , Contagem de Leucócitos/métodos , Masculino , Índice de Gravidade de Doença , Estatísticas não ParamétricasRESUMO
The mitochondrial DNA variation of 295 Berber-speakers from Morocco (Asni, Bouhria and Figuig) and the Egyptian oasis of Siwa was evaluated by sequencing a portion of the control region (including HVS-I and part of HVS-II) and surveying haplogroup-specific coding region markers. Our findings show that the Berber mitochondrial pool is characterized by an overall high frequency of Western Eurasian haplogroups, a somehow lower frequency of sub-Saharan L lineages, and a significant (but differential) presence of North African haplogroups U6 and M1, thus occupying an intermediate position between European and sub-Saharan populations in PCA analysis. A clear and significant genetic differentiation between the Berbers from Maghreb and Egyptian Berbers was also observed. The first are related to European populations as shown by haplogroup H1 and V frequencies, whereas the latter share more affinities with East African and Nile Valley populations as indicated by the high frequency of M1 and the presence of L0a1, L3i, L4*, and L4b2 lineages. Moreover, haplogroup U6 was not observed in Siwa. We conclude that the origins and maternal diversity of Berber populations are old and complex, and these communities bear genetic characteristics resulting from various events of gene flow with surrounding and migrating populations.