Synergistic action of phages and lytic proteins with antibiotics: a combination strategy to target bacteria and biofilms.

BACKGROUND: Multidrug-resistant bacteria continue to emerge owing to the abuse of antibiotics and have a considerable negative impact on people and the environment. Bacteria can easily form biofilms to improve their survival, which reduces the efficacy of antibacterial drugs. Proteins such as endolysins and holins have been shown to have good antibacterial activity and effectively removal bacterial biofilms and reduce the production of drug-resistant bacteria. Recently, phages and their encoded lytic proteins have attracted attention as potential alternative antimicrobial agents. The aim of the present study was to investigate the sterilising efficacy of phages (SSE1, SGF2, and SGF3) and their encoded lytic proteins (lysozyme and holin), and to further explore their potential in combination with antibiotics. To the ultimate aim is to reduce or replace the use of antibiotics and provide more materials and options for sterilisation. RESULTS: Phages and their encoded lytic proteins were confirmed to have great advantages in sterilisation, and all exhibited significant potential for reducing bacterial resistance. Previous studies on the host spectrum demonstrated the bactericidal efficacy of three Shigella phages (SSE1, SGF2, and SGF3) and two lytic proteins (LysSSE1 and HolSSE1). In this study, we investigated the bactericidal effects on planktonic bacteria and bacterial biofilms. A combined sterilisation application of antibiotics, phages, and lytic proteins was performed. The results showed that phages and lytic proteins had better sterilisation effects than antibiotics with 1/2 minimum inhibitory concentrations (MIC) and their effect was further improved when used together with antibiotics. The best synergy was shown when combined with ß- lactam antibiotics, which might be related to their mechanism of sterilising action. This approach ensures a bactericidal effect at low antibiotic concentrations. CONCLUSIONS: This study strengthens the idea that phages and lytic proteins can significantly sterilise bacteria in vitro and achieve synergistic sterilisation effects with specific antibiotics. Therefore, a suitable combination strategy may decrease the risk of drug resistance.

Testosterone deficiency in non-obese type 2 diabetic male patients.

BACKGROUND AND AIMS: it is unclear whether male hypogonadism is ascribable to the diabetic state per se, or because of other factors, such as obesity or age. We aimed to investigate the prevalence and identify the predictors for testosterone deficiency among non-obese type 2 diabetic males. METHODS: This cross-sectional study was conducted on 95 nonobese type 2 diabetic males with BMI below 30. We evaluated the total testosterone (TT) levels to determine prevalence and risk factors of testosterone deficiency. Serum TT ≤ 300 ng/dl defined testosterone deficiency. RESULTS: The prevalence of testosterone deficiency was 29.1%. Testosterone deficient patients had statistically significantly higher visceral adiposity index (VAI), waist, and triglyceride in comparison with normal testosterone patients. TT level correlated with VAI, waist, BMI, LH, and age. VAI was the only significant predictor of TT levels even after adjustment for age and BMI in regression analysis. Furthermore, VAI was a statistically significant risk factor for testosterone deficiency in binary logistic analysis. CONCLUSIONS: testosterone deficient non-obese type 2 diabetic male patients had elevated VAI, waist, and triglyceride. Moreover, elevated VAI was a risk factor for testosterone deficiency. VAI could be an easily applicable and reliable index for the evaluation and prediction in type 2 non-obese diabetic males.

Inorganic-organic mesoporous hybrid segregators for selective and sensitive extraction of precious elements from urban mining.

This study aimed to develop a highly selective extraction protocol for gold (AuIII) ions from electronic urban waste (EUW) using simple, low-cost Inorganic-organic mesoporous hybrid segregators. The unique features of mesoporous hybrid segregator architectures are of particular to ensure effective adsorption system in terms of selective and sensitive recovery of AuIII ions from EUW. The segregator platform featured 3D micrometric, mesocage double-serrated plant-leaf-like γ-Al2O3 sheets with hierarchy surfaces containing tri-modal mesopores interiorly and uniformly arranged toothed edges of ~20-40 and ~15 nm groove width and depth at the exterior surfaces, respectively. Rational incorporation of actively organic chelates into hierarchical γ-Al2O3 sheet platforms leads to the production of a couple of selective segregators 1 and 2 (namely, SC1 and SC2) for AuIII ions at specific conditions by applying batch and fixed-bed columnar techniques. The mesocage SC segregators offer a selective extraction approach of AuIII ions from mixed element contents released from a computer motherboard (CMB). Our finding indicated that the textural and hierarchal features of the mesocage SC segregators played key roles in the selective adsorption/recovery of AuIII ions at pH 2-2.5 with high capacity (136-141 mg/g range) and effective reusability ≫10 consecutive cycles. In general, the developed SCs could be utilized as a real extractor of AuIII recovery from spent CMBs.