RESUMO
We associated Laguna Negra virus with hantavirus pulmonary syndrome in Mato Grosso State, Brazil, and a previously unidentified potential host, the Calomys callidus rodent. Genetic testing revealed homologous sequencing in specimens from 20 humans and 8 mice. Further epidemiologic studies may lead to control of HPS in Mato Grosso State.
Assuntos
Síndrome Pulmonar por Hantavirus/veterinária , Orthohantavírus/genética , Doenças dos Roedores/virologia , Animais , Arvicolinae , Teorema de Bayes , Brasil/epidemiologia , Variação Genética , Orthohantavírus/classificação , Síndrome Pulmonar por Hantavirus/epidemiologia , Síndrome Pulmonar por Hantavirus/virologia , Humanos , Funções Verossimilhança , Camundongos , Tipagem de Sequências Multilocus , Proteínas do Nucleocapsídeo/genética , Filogenia , Doenças dos Roedores/epidemiologiaRESUMO
To study the dynamics of wild rodent populations and identify potential hosts for hantavirus, we conducted an eco-epidemiologic study in Campo Novo do Parecis, Mato Grosso State, Brazil. We detected and genetically characterized Castelo dos Sonhos virus found in a species of pygmy rice rat (Oligoryzomys utiaritensis).
Assuntos
Reservatórios de Doenças/veterinária , Infecções por Hantavirus/veterinária , Orthohantavírus/genética , Doenças dos Roedores/epidemiologia , Sigmodontinae/virologia , Animais , Anticorpos Antivirais/sangue , Brasil/epidemiologia , DNA Viral/análise , Reservatórios de Doenças/virologia , Orthohantavírus/imunologia , Orthohantavírus/isolamento & purificação , Infecções por Hantavirus/epidemiologia , Infecções por Hantavirus/virologia , Filogenia , Doenças dos Roedores/virologia , Análise de Sequência de DNARESUMO
To confirm circulation of Anajatuba virus in Maranhao, Brazil, we conducted a serologic survey (immunoglobulin G ELISA) and phylogenetic studies (nucleocapsid gene sequences) of hantaviruses from wild rodents and persons with hantavirus pulmonary syndrome. This virus is transmitted by Oligoryzomys fornesi rodents and is responsible for hantavirus pulmonary syndrome in this region.
Assuntos
Reservatórios de Doenças/virologia , Monitoramento Ambiental , Síndrome Pulmonar por Hantavirus/epidemiologia , Orthohantavírus/classificação , Sigmodontinae/virologia , Adulto , Animais , Anticorpos Antivirais/sangue , Brasil/epidemiologia , Busca de Comunicante , Estudos Transversais , Monitoramento Epidemiológico , Feminino , Orthohantavírus/genética , Orthohantavírus/isolamento & purificação , Síndrome Pulmonar por Hantavirus/sangue , Síndrome Pulmonar por Hantavirus/veterinária , Humanos , Imunoglobulina G/sangue , Masculino , Dados de Sequência Molecular , Filogenia , RNA Viral/análise , RNA Viral/genética , Estudos SoroepidemiológicosRESUMO
Following the occurrence of the first laboratory-confirmed cases of hantavirus pulmonary syndrome (HPS) in Maranhao State, Brazil, rodents were trapped and rodent materials screened by ELISA for antibodies to Sin Nombre and Andes hantaviruses. Antibody-positive samples were tested by RT-PCR, amplified products were sequenced, and phylogenetic trees were constructed for comparison with known hantaviruses. From 104 rodent blood samples collected (40 Bolomys lasiurus, 52 Holochilus sciureus, 12 Oligoryzomys fornesi, and one Proechimys guyannensis), 21 (20.2%) were antibody-positive (one B. lasiurus, five O. fornesi, and 15 H. sciureus). Hantavirus RNA was amplified by PCR from two O. fornesi and four H. sciureus. Viral sequencing identified two hantavirus genotypes. The genotype recovered from O. fornesi, is designated herein as Anajatuba (ANAJ) and the genotype recovered from H. sciureus is designated Rio Mearim (RIME). Phylogenetic analysis of a 643-nucleotide region of the N segment showed both viruses to be most closely related (94-96% nucleotide homology) to Río Mamoré virus, a virus associated with Oligoryzomys microtis in Bolivia and Peru, but not found in northern Brazil. O. fornesi was frequently captured in and around human dwellings. H. sciureus, is a semi-aquatic rodent captured only in remote areas rarely frequented by humans.
Assuntos
Síndrome Pulmonar por Hantavirus/virologia , Orthohantavírus/genética , Roedores/virologia , Sequência de Aminoácidos , Animais , Anticorpos Antivirais/sangue , Sequência de Bases , Brasil , DNA Viral/química , DNA Viral/genética , Reservatórios de Doenças/veterinária , Ensaio de Imunoadsorção Enzimática , Genótipo , Orthohantavírus/classificação , Orthohantavírus/imunologia , Orthohantavírus/isolamento & purificação , Síndrome Pulmonar por Hantavirus/transmissão , Humanos , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência do Ácido Nucleico , ZoonosesRESUMO
In 2000, the first outbreak of hantavirus pulmonary syndrome was recognized in the Brazilian Amazon (Maranhao State). An epidemiologic study identified a 13.3% prevalence of hantavirus-specific immunoglobulin G. The analysis of risk factors suggests that persons are occupationally exposed to infected rodents in the crop fields.
Assuntos
Anticorpos Antivirais/sangue , Síndrome Pulmonar por Hantavirus/epidemiologia , Imunoglobulina G/sangue , Orthohantavírus/imunologia , Adolescente , Adulto , Idoso , Agricultura , Animais , Brasil/epidemiologia , Feminino , Síndrome Pulmonar por Hantavirus/virologia , Humanos , Masculino , Pessoa de Meia-Idade , Exposição Ocupacional , Prevalência , Fatores de Risco , Doenças dos Roedores/virologia , Roedores/virologiaRESUMO
The authors report a confirmed case of hantavirus pulmonary syndrome in the rural area of the municipality of Anajatuba, state of Maranhäo. Two other suspected cases from the same region are also described. The confirmed case involved a previously healthy young woman who died with signs and symptoms of acute respiratory insufficiency 5 days after presenting fever, myalgia and a dry cough. The patient was a student who was helping her parents with work in the fields; it was a habit of the family to store rice inside the house. The suspected cases involved two first-degree relatives working as field hands who died of acute respiratory insufficiency 24 and 48 hours, respectively, after presenting fever, myalgia and a dry cough. Both stored rice and corn inside their home. People living in the region reported massive infestations with rats in the woods and fields