Assessment of hematological indicators, histological alterations, and DNA damage in Danube sturgeon (Acipenser gueldenstaedtii) exposed to the organophosphate malathion.

This study focused on organophosphate malathion toxicity in Danube sturgeon (Acipenser gueldenstaedtii) and its negative effects in sub-lethal concentration. In this context, the LC50 value of fish exposed to five different concentrations and two different sub-lethal concentrations depending on the LC50 value were considered. Accordingly, LC50 of malathion for 96 h was 3.24 mg L-1. In leukocyte (WBC) and hemoglobin (HGB) such as hematological indicators, significant differences were observed in sub-lethal concentration (One-eighth of the LC50 =0.4 mg L-1). In addition, serious histological alterations were observed in the gill and liver tissues after both acute (96 h) and chronic periods (28 days). While epithelial lifting and hyperplasia were the most prominent lesions in the gill, intense vacuolization were observed in the liver. In addition, circulatory disturbances, regressive changes, and progressive changes in the gill and circulatory disturbances and inflammation in the liver were significantly higher for the chronic period. Finally, significantly higher DNA damage was detected in fish exposed malathion in the chronic period compared to the control group. In conclusion, the present study has proven that malathion has a hematological, histological and genotoxic effect on the endangered species A. gueldenstaedtii. Thus, the current study will motivate for residue studies on A. gueldenstaedtii and trigger conservation strategies for local governments.

Decade of microplastic alteration in the southeastern black sea: An example of seahorse gastrointestinal tracts.

Unconscious and excessive use of plastic supports the diversity and abundance of microplastics (MPs) in marine environments. As a result of MP exposure, organisms in the marine environment are faced with adverse scenarios up to death. In this study, ten-year MP composition was investigated in gastrointestinal tracts (GITs) of low-mobility seahorses (90 individuals per period) from the Southeastern Black Sea. Seahorse GITs sampled during both 2012 and 2022 contain 102 and 135 MP items, respectively. The number of MPs per unit individual seahorse and unit seahorse weight was higher in the 2022 period. On the other hands, no significant differences were observed between the MP lengths of both periods. The majority of MPs in both sample periods were materials shorter than 1000 µm. Of the eight found synthetic polymers, five belonged to the 2012 period, while seven were observed during the 2022 period. Additionally, the most abundant synthetic polymer for both periods is polyvinyl stearate (PVS). As a result, 43% of the total plastic material belonged to the 2012 period, while 57% was observed in the 2022 period. Considering both the diversity of polymers and the abundance of plastics, the region was adversely affected by plastic materials in the 2022 period.

Investigation of long-term bisphenol A exposure on rainbow trout (Oncorhynchus mykiss): Hematological parameters, biochemical indicator, antioxidant activity, and histopathological examination.

As an industrial synthetic chemical, bisphenol A (BPA) has the potential to have physiologically and histologically adverse effects on aquatic organisms. BPA causes the reproductive disrupting of all vertebrates due to its degradation on endocrine system. Therefore, the effect of BPA on fish with high economic value is an important issue. This study focused weekly on long-term BPA exposure on rainbow trout (Oncorhynchus mykiss). Hematological, biochemical, antioxidant activity and histopathological examinations were performed on O. mykiss exposed to 1000 µg/L BPA concentration. Mortality was observed in the BPA group during the first three weeks. As a result of hematological studies, leukocyte count and hemoglobin in the BPA group were significantly higher in the first three weeks compared to the control group. Plasma cortisol level as a biochemical indicator showed a similar trend to leukocyte and hemoglobin. There was no significant difference between BPA and control groups in terms of superoxide dismutase and catalase. However, glutathione peroxidase activity in the BPA group was significantly lower than in the control group for four weeks. At the end of the study, many alterations were observed histologically in the gill tissues. While more intense hyperplasia and epithelial lifting were observed in the tissues in the BPA group compared to the control group, vacuolization, necrosis and hypertrophy were observed infrequently. In conclusion, this study argues that BPA causes negative effects on O. mykiss in terms of fish welfare and future study should be focused on its environmental adaptation with color preference patterns.

Acute Toxicity of Pozzolanic Cement on Two Crustacean Species, Water Flea (Daphnia magna) and Gammarus komareki.

This study was conducted to determining the values of LC50, mortality rates and DNA damages (Daphnia magna) of species exposed to pozzolanic cement concentrations of 50, 100, 150, 200, 250, and 300 mg/L for 24 h. To compare the effect of increased pH value associated with the addition of cement, a NaOH group was also formed in Gammarus komareki individuals. As a result, the LC50 values in D. magna and G. komareki were calculated as 118.57 and 197.24 mg/L/24 h, respectively. It was observed that, unlike the G. komareki individuals, cement particles were accumulated on D. magna. In the comparison trial (NaOH) performed on G. komareki individuals, 60% mortality was determined. The number of deaths from cement and NaOH in the experimental groups with the same pH values were found similar. There was not statistically significant difference between control and experimental groups for DNA damage on D. magna. As a result, it has been determined that cement has a toxic effect on D. magna and G. komareki due to increasing the pH value of water.