Unravelling the halophyte Suaeda maritima as an efficient candidate for phytostabilization of cadmium and lead: Implications from physiological, ionomic, and metabolomic responses.

Cadmium (Cd) and lead (Pb) are among the most toxic heavy metals affecting human health and crop yield. Suaeda maritima (L.) Dumort is an obligate halophyte that is well adapted to saline soil. The inbuilt salinity tolerance mechanisms of halophytes help them to survive in heavy metal-contaminated rhizospheric soil. In the present study, growth and ionomic responses, reactive oxygen species (ROS) accumulation, modulations of phytochelatins, antioxidative defense, and metabolomic responses were studied in S. maritima imposed to Cd and Pb stresses with an aim to elucidate Cd and Pb tolerance mechanisms and phytoremediation potential of this halophyte. Our results showed a reduction of biomass in S. maritima, which may serve as an energy conservation strategy for survival under heavy metal stress. The increased accumulation of ROS with concomitant higher expression of various antioxidative enzymes suggests the efficient scavenging of ROS. The metabolite profiling revealed significant up-regulation of sugars, sugar alcohols, amino acids, polyphenols, and organic acids under Cd and Pb stresses suggesting their possible role in osmotic balance, ionic homeostasis, ROS scavenging, and signal transduction for stress tolerance. In S. maritima, the translocation factors (Tf) are <1 in both Cd and Pb treatments, which indicates that this halophyte has high phytostabilization potential for Cd and Pb in roots and through restricted translocation of heavy metal ions to the aboveground part. The findings of this study offer comprehensive information on Cd and Pb tolerance mechanisms in S. maritima and suggest that this halophyte can detoxify the HMs through physiological, ionic, antioxidative, and metabolic regulations.

Regulation of chromium translocation to shoot and physiological, metabolomic, and ionomic adjustments confer chromium stress tolerance in the halophyte Suaeda maritima.

Chromium (Cr) is a highly toxic element adversely affecting the environment, cultivable lands, and human populations. The present study investigated the effects of Cr (VI) (100-400 µM) on plant morphology and growth, photosynthetic pigments, organic osmolytes, ionomics, and metabolomic dynamics of the halophyte Suaeda maritima to decipher the Cr tolerance mechanisms. Cr exposure reduced the growth and biomass in S. maritima. The photosynthetic pigments content significantly declined at higher Cr concentrations (400 µM). However, at lower Cr concentrations (100-300 µM), the photosynthetic pigments remained unaffected or increased. The results suggest that a high concentration of Cr exposure might have adverse effects on PS II in S. maritima. The enhanced uptake of Na+ in S. maritima imposed to Cr stress indicates that Na+ might have a pivotal role in osmotic adjustment, thereby maintaining water status under Cr stress. The proline content was significantly upregulated in Cr-treated plants suggesting its role in maintaining osmotic balance and scavenging ROS. The metabolomic analysis of control and 400 µM Cr treated plants led to the identification of 62 metabolites. The fold chain analysis indicated the upregulation of several metabolites, including phytohormones (SA and GA3), polyphenols (cinnamic acid, sinapic acid, coumaric acid, vanillic acid, and syringic acid), and amino acids (alanine, leucine, proline, methionine, and cysteine) under Cr stress. The upregulation of these metabolites suggests the enhanced metal chelation and sequestration in vacuoles, reducing oxidative stress by scavenging ROS and promoting photosynthesis by maintaining the chloroplast membrane structure and photosynthetic pigments. Furthermore, in S. maritima, Cr tolerance index (Ti) was more than 60% in all the treatments, and Cr bio-concentration factor (BCF) and translocation factor (Tf) values were all greater than 1.0, which clearly indicates the Cr-hyperaccumulator characteristics of this halophyte.

Potassium deficiency stress tolerance in peanut (Arachis hypogaea) through ion homeostasis, activation of antioxidant defense, and metabolic dynamics: Alleviatory role of silicon supplementation.

Potassium (K) scarcity of arable land is one of the important factors that hamper the growth of the plants and reduce yield worldwide. In the current study, we examine the physiological, biochemical, and metabolome response of Arachis hypogaea (GG7 genotype: fast-growing, tall, early maturing, and high yielding) under low K either solitary or in combination with Si to elucidate the ameliorative role of Si. The reduced fresh and dry biomass of peanut and photosynthetic pigments content was significantly alleviated by Si. Si application did not affect the leaf and stem K+, although it enhanced root K+ in K-limitation, which is probably due to up-regulated expression of genes responsible for K uptake. Si improves the potassium use efficiency in K-limitation as compared to control. K-deficiency increased MDA, O2•-, and H2O2 levels in leaf and root of peanut. Si improved/maintained the activity of antioxidative enzymes, which significantly lowered the ROS accumulation in K-limitation. The AsA/DHA and GSH/GSSG ratio was approximately unaffected in both leaf and root, suggesting the maintained cellular redox potential in K-starved peanut. Si promotes accumulation of sugars and sugar alcohols, phytohormones indicating their probable involvement in signal transduction, osmotic regulation, and improvement of stress tolerance. Down-regulation of aspartic acid and glutamic acid while up-regulation of lysine, histidine, and arginine could maintain charge balance in K-deprived peanut. The significant accumulation of polyphenols under K limitation supplemented with Si suggests the role of polyphenols for ROS scavenging. Our results demonstrated that Si as a beneficial element can mitigate K-nutrient toxicity and improve KUE of peanut under K-limitation conditions. Moreover, our results demonstrate that Si application can improve crop yield, quality, and nutrient use efficiency under nutrient limitation conditions.

Silicon-induced mitigation of drought stress in peanut genotypes (Arachis hypogaea L.) through ion homeostasis, modulations of antioxidative defense system, and metabolic regulations.

Drought stress considered as a major environmental constraint that frequently limits crop production globally. In the current investigation, drought stress-induced alterations in growth, ion homeostasis, photosynthetic pigments, organic osmolytes, reactive oxygen species (ROS) generation, antioxidative components, and metabolic profile were examined in order to assess the role of silicon (Si) in mitigation of drought effects and to understand the drought adaptive mechanism in two contrasting peanut genotypes (GG7: fast growing and tall, TG26: slow growing and semi-dwarf). Si application significantly improved the leaf chlorophyll content, relative water content % (RWC %), growth and biomass in GG7 compared with TG26 genotype under water stress. Si supplementation considerably promotes the uptake and transport of mineral nutrients under drought condition in both the genotypes, which eventually promote plant growth. Exogenous application of Si protects the photosynthetic pigments from oxidative damage by reducing membrane lipid peroxidation and either maintained or reduced H2O2 accumulation in both the genotypes. The activity of enzymatic antioxidants like superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), and glutathione reductase (GR) and non-enzymatic antioxidants like ascorbate (AsA) and glutathione (GSH) were either maintained or increased in both the genotypes in response to Si under drought as compared to those without Si. Silicon-induced higher accumulation of metabolites mainly sugars and sugar alcohols (talose, mannose, fructose, sucrose, cellobiose, trehalose, pinitol, and myo-inositol), amino acids (glutamic acid, serine, histidine, threonine, tyrosine, valine, isoleucine, and leucine) in GG7 genotype as compared to TG26, provides osmo-protection. Moreover, Si application increased phytohormones levels such as indole-3-acetic acid (IAA), gibberellic acid (GA3), jasmonic acid (JA), and zeatin in GG7 genotype under drought stress compared to non-Si treated seedlings suggesting its involvement in signaling pathways for drought adaptation and tolerance. Noteworthy increment in polyphenols (chlorogenic acid, caffeic acid, ellagic acid, rosmarinic acid, quercetin, coumarin, naringenin, and kaempferol) in the Si treated seedlings of GG7 genotype as compared to TG26 under drought stress suggests an efficient mechanism of ROS sequestration in GG7 genotype. Our findings provide comprehensive information on physiological, biochemical, and metabolic dynamics associated with Si-mediated water stress tolerance in peanut. This study indicates that the drought tolerance efficacy of peanut genotypes can be improved by Si application.