[Selected elements of biological activity of circulating immune complexes in serum of patients with atopic diseases. I. Ability to injure guinea pig tissue by injecting atopic patient plasma]. / Wybrane elementy aktywnosci biologicznej krazacych kompleksów immunologicznych w surowicy chorych atopowych. I. Zdolnosc uszkodzenia tkanek skóry swinek morskich przez surowice chorych atopowych.

Serum level was measured of circulating complexes (SpA binding method-CIC/SpA) in pollen and house dust mite sensitive patients during specific immunotherapy. Histopathological changes in guinea pigs skin after the intradermal injection of patients serum have been studied. Differences were demonstrated in the observed patho-morphological changes in relation to CICs/SpA serum concentrations.

[Clinical and patho-morphological evaluation of patients dying from bronchial asthma]. / Ocena kliniczna i patomorfologiczna chorych zmarlych na astme oskrzelowa.

Mortality from asthma within 1978-1987 was analysed retrospectively. This analysis included selected factors determining the mortality in this disease. Some conditions being able to decrease death risk in asthmatic patients were defined. The results of autopsies of patients who died from asthma were also analysed with an attempt to interpret those results basing on the selected clinical data. Unified scheme of sampling for pathomorphological examinations was proposed together with proper interpretation of histopathologies in the lungs and myocardium. Presented data are preliminary and will be continued in foreseen prospective studies.

Biological properties and clinical application of propolis. VII. Investigation of immunogenic properties of ethanol extract of propolis (EEP).

Under experimental conditions parenteral administration of ethanol extract of propolis (EEP) solutions to rabbits induced no synthesis of antibodies as investigated by means of ring precipitation, double diffusion gel precipitation and complement fixation test.

A trial of using antibodies as carriers of alkylating agents. II. Evaluation of ability to form 32P-cyclophosphamide + immune antibody complexes with homologous antigen.

32P-cyclophosphamide was found to combine with gamma-globulin fractions of immune sera. Immune sera incubated with 32P-cyclophosphamide retained ability to react specifically with homologous antigen in vitro in the system: MN antigens of human erythrocytes + rabbit anti-MN antibody, and probably reacted selectively with target antigens in vivo in the system: antigens of guinea pig kidney tissue + rabbit antibodies against these antigens. Hemagglutination, passive hemagglutination and precipitation in agar gel tests were used in the experiments. Ability to combine of the immune antibody + 32P-cyclophosphamide complex with homologous antigens was evaluated by measurements of radioactivity of studied materials (erythrocyte agglutinates and organ homogenates). The results indicate feasibility of using immune antibodies as carriers of cytostatic agents.

Studies on stimulation and properties of antiglobulin antibodies. II. Antiglobulin antibodies against homologous globulins and antigens of thermally denatured autologous sera in the sera of rabbits immunized with various antigens.

Sera of 48 rabbits after long-term immunization with suspensions of Diplococcus pneumoniae, Klebsiella friedländeri, Proteus OX19 and solution of bovine albumin were examined for antibodies reacting with homologous gamma-globulins and antigens of thermally denatured autologous sera. Appearance of antibodies of this type was related to duration of immunization, but not related to the type of antigen used. The experimental results were explained as a result of altered antigenic properties of antibodies following reaction with homologous antigen.

Studies on stimulation and properties of antiglobulin antibodies. I. Influence of heatig at 56 degrees C and 60 degrees C on antigenicity.

The autoimmunogenic properties of whole rabbit serum heated at 56 degrees C and 60 degrees C were studied in rabbits. Autologous rabbit serum heated 20 min at 60 degrees C was immunogenic. The state of induced immunity manifested itself by the appearance of antibodies to antigens of heterologous sera: human, goat and guinea pig. No antibodies for autologous sera were found, including autologous sera heated 20 min at 60 degrees C. The antibodies for heterologous sera directed against gamma-globulins were detected by the passive hemagglutination test and by precipitation in agar gel.

Studies on stimulation and properties of antiglobulin antibodies. III. Antiglobulin antibodies with different specificity in patients with bronchial asthma complicated by suppurative catarrh of the respiratory tract.

Sera from 14 of 50 patients suffering from bronchial asthma complicated by suppurative catarrh of the respiratory tract contained antibodies to homologous and heterologous gamma-globulins. The findings were attributed to hyperimmunization causing changed in antigenic properties of antibodies. Antigenically altered antibodies can act as autoimmunogenic stimuli inducing an immune response manifested by the appearance of antibodies to autologous gamma-globulins and cross reacting with homologous and heterologous gamma-globulins.

A trial of utilizing antibodies as carriers of alkylating agents. I. Influence of cyclophosphamide and chlormethine on antigenic properties of serum proteins.

Electrophorograms of human and rabbit sera before and after incubation with various concentrations of cyclophosphamide and chlormethine (Nitrogranulogen) were compared. Sera of rabbits injected with chlormethine and cyclophosphamide in therapeutic doses for humans were examined for antibodies reacting with homologous and antologous gamma-globulins. Immunoelectrophorograms of sera incubated with both alkylating agents differed from those of preincubation sera with regard to number and intensity of precipitin lines. The changes in electrophorograms were related to the type and concentration of alkylating agent. No antibodies to homologous or autologous serum antigens of rabbits treated with chlormethine or cyclophosphamide were found.