Whole genome sequencing data of Chromobacterium amazonense BASUSDA_45 isolated from soil in Bangladesh capable of degrading pesticide.

This article reports the Chromobacterium amazonense BASUSDA_45 strain's draft genomic sequence. The bacterium was isolated from cypermethrin pesticide contaminated soil and then the sequencing was carried out. Initially de novo assembly of the raw sequences, trimming and quality check generates 125 contigs having N50 of 78,923. Further mapping of the contigs generated scaffolds. The genome contains 53 scaffolds with a total length of 4,295,151 bp having 62.30% GC content and N50 of 3,726,017. Annotation using Prokaryotic Genome Annotation Pipeline (PGAP) reveals 4181 genes among which 4096 were coding sequences, 76 tRNAs, 3 rRNAs, 4 noncoding RNAs. The raw sequence reads and annotated genome were uploaded to NCBI's Bioproject repository with the accession number PRJNA686506.

Characterization and evaluation of antibacterial and antiproliferative activities of crude protein extracts isolated from the seed of Ricinus communis in Bangladesh.

BACKGROUND: Ricinus communis (Euphorbiaceae) has previously been reported to possess analgesic, antihistamine, antioxidant and anti-inflammatory activities. This study was designed for isolation, characterization and evaluation of antibacterial and anti-proliferative activities of R. communis seed protein. METHODS: The concentration and molecular weight of R. communis seed protein were estimated by SDS-PAGE and spectrophotometric analysis, respectively. Lectin activity was evaluated by hemagglutination assay on mice blood. In vitro susceptibility of four human pathogenic bacteria including Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes and Staphylococcus aureus was detected using disk diffusion assay, and minimum inhibitory concentration (MIC) value was determined using micro-dilution method. A total of twenty four Swiss albino mice containing Ehrlich's ascites carcinoma (EAC) cells were treated with the crude protein of R. communis at 50 and 100 µg/ml/d/mouse for 6 days. Growth inhibitory activity of R. communis seed protein on EAC cells was determined by haemocytometer counting using trypan blue dye and DAPI (4΄,6-diamidino-2-phenylindole) staining was used to assess apoptotic cells. RESULTS: The protein concentration of six R. communis (castor) varieties ranged between 21-35 mg/ml and molecular weight between 14-200 kDa. Castor protein agglutinated mice blood at 3.125 µg/wall. The seed protein shows considerable antimicrobial activity against E. coli, P. aeruginosa and S. aureus, exhibiting MIC values of 250, 125 and 62.5 µg/ml, respectively. Administration of seed protein led to 54 % growth inhibition of EAC cells at 100 µg/ml. DAPI staining indicates marked features of apoptosis including condensation of cytoplasm, nuclear fragmentation and aggregation of apoptotic bodies etc. CONCLUSION: Our study suggests that the lectin rich R. communis seed protein has strong antibacterial and anticancer activities.