Isolated and combined effects of thermal stress and copper exposure on the trophic behavior and oxidative status of the reef-building coral Mussismilia harttii.

Global warming and local disturbances such as pollution cause several impacts on coral reefs. Among them is the breakdown of the symbiosis between host corals and photosynthetic symbionts, which is often a consequence of oxidative stress. Therefore, we investigated if the combined effects of thermal stress and copper (Cu) exposure change the trophic behavior and oxidative status of the reef-building coral Mussismilia harttii. Coral fragments were exposed in a mesocosm system to three temperatures (25.0, 26.6 and 27.3 °C) and three Cu concentrations (2.9, 5.4 and 8.6 µg L-1). Samples were collected after 4 and 12 days of exposure. We then (i) performed fatty acid analysis by gas chromatography-mass spectrometry to quantify changes in stearidonic acid and docosapentaenoic acid (autotrophy markers) and cis-gondoic acid (heterotrophy marker), and (ii) assessed the oxidative status of both host and symbiont through analyses of lipid peroxidation (LPO) and total antioxidant capacity (TAC). Our findings show that trophic behavior was predominantly autotrophic and remained unchanged under individual and combined stressors for both 4- and 12-day experiments; for the latter, however, there was an increase in the heterotrophy marker. Results also show that 4 days was not enough to trigger changes in LPO or TAC for both coral and symbiont. However, the 12-day experiment showed a reduction in symbiont LPO associated with thermal stress alone, and the combination of stressors increased their TAC. For the coral, the isolated effects of increase in Cu and temperature led to an increase in LPO. The effects of combined stressors on trophic behavior and oxidative status were not much different than those from the isolated effects of each stressor. These findings highlight that host and symbionts respond differently to stress and are relevant as they show the physiological response of individual holobiont compartments to both global and local stressors.

Artificial light at night (ALAN) alters the physiology and biochemistry of symbiotic reef building corals.

Artificial Light at Night (ALAN), which is the alteration of natural light levels as the result of anthropogenic light sources, has been acknowledged as an important factor that alters the functioning of marine ecosystems. Using LEDs light to mimic ALAN, we studied the effect on the physiology (symbiont and chlorophyll contents, photosynthesis, respiration, pigment profile, skeletal growth, and oxidative stress responses) of two scleractinian coral species originating from the Red Sea. ALAN induced the photoinhibition of symbiont photosynthesis, as well as an overproduction of reactive oxygen species (ROS) and an increase in oxidative damage to lipids in both coral species. The extent of the deleterious effects of ALAN on the symbiotic association and coral physiology was aligned with the severity of the oxidative stress condition experienced by the corals. The coral species Sylophora pistillata, which experienced a more severe oxidative stress condition than the other species tested, Turbinaria reniformis, also showed a more pronounced bleaching (loss of symbionts and chlorophyll content), enhanced photoinhibition and decreased photosynthetic rates. Findings of the present study further our knowledge on the biochemical mechanisms underpinning the deleterious impacts of ALAN on scleractinian corals, ultimately shedding light on the emerging threat of ALAN on coral reef ecology. Further, considering that global warming and light pollution will increase in the next few decades, future studies should be taken to elucidate the potential synergetic effects of ALAN and global climate change stressors.

Unravelling the different causes of nitrate and ammonium effects on coral bleaching.

Mass coral bleaching represents one of the greatest threats to coral reefs and has mainly been attributed to seawater warming. However, reduced water quality can also interact with warming to increase coral bleaching, but this interaction depends on nutrient ratios and forms. In particular, nitrate (NO3-) enrichment reduces thermal tolerance while ammonium (NH4+) enrichment tends to benefit coral health. The biochemical mechanisms underpinning the different bleaching responses of corals exposed to DIN enrichment still need to be investigated. Here, we demonstrated that the coral Stylophora pistillata underwent a severe oxidative stress condition and reduced aerobic scope when exposed to NO3- enrichment combined with thermal stress. Such condition resulted in increased bleaching intensity compared to a low-nitrogen condition. On the contrary, NH4+ enrichment was able to amend the deleterious effects of thermal stress by favoring the oxidative status and energy metabolism of the coral holobiont. Overall, our results demonstrate that the opposite effects of nitrate and ammonium enrichment on coral bleaching are related to the effects on corals' energy/redox status. As nitrate loading in coastal waters is predicted to significantly increase in the future due to agriculture and land-based pollution, there is the need for urgent management actions to prevent increases in nitrate levels in seawater. In addition, the maintenance of important fish stocks, which provide corals with recycled nitrogen such as ammonium, should be favoured.