Detection of Staphylococcus aureus including MRSA on environmental surfaces in a jail setting.

We examined jail environmental surfaces to explore whether they might serve as reservoirs of viable methicillin-resistant Staphylococcus aureus (MRSA). We swabbed 132 surfaces, inoculated primary and secondary mannitol salts and oxacillin-resistant screening agar, and used API tests to identify S. aureus and E-tests to determine methicillin/oxacillin resistance. We recovered S. aureus from 10 (7.6%) surfaces; eight (6.1%) isolates were MRSA. We ran pulsed-field gel electrophoresis on six resistant isolates and observed three patterns, one of which was identical to that identified in a previous study of inmates' nasal specimens. Finding MRSA-contaminated surfaces on a variety of environmental surfaces in the absence of an overt outbreak emphasizes that correctional facilities should have protocols for environmental cleaning as a component of MRSA prevention.

Microbial evaluation of foodservice surfaces in Texas child-care centers.

Children under the age of 5 years experience a disproportionately high rate of bacterial enteric infections. Research has shown a relationship between inadequate child-care center sanitation and illnesses in children. This cross-sectional study assessed the sanitation levels of foodservice surfaces in a sample of 36 Texas child-care centers via recovery and identification of selected enteric gram-negative bacteria. The centers in this study had the capacity to care for 50 to 332 children and represented diverse socioeconomic and racial profiles. Forty-one percent (68 of 167) of total swab samples collected tested positive for bacteria. Twenty-seven different types of bacteria were identified from positive swab samples. Most of the bacteria found are considered opportunistic pathogens, which can pose serious health risks to those with compromised immune systems, such as young children. Two types of bacteria recovered, Salmonella paratyphi A and Klebsiella pneumonia, are considered nonopportunistic and can infect healthy individuals. The most common areas of bacterial contamination were the sink drain area of the dishwashing sink, the hand-washing sink faucet handles, the handle of the garbage can lid, and cutting boards. It is vital for child-care staff to wash their hands often and disinfect all surfaces, because even surfaces that appear clean can harbor microorganisms.