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J Vet Intern Med ; 28(5): 1405-13, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24990398

RESUMO

BACKGROUND: Portable blood glucose meters (PBGMs) allow easy glucose measurements. As animal-specific PBGMs are not available everywhere, those for humans are widely used. OBJECTIVES: To assess the accuracy and precision of 9 PBGMs in canine whole blood (WB) and plasma, based on the ISO 15197:2013. ANIMALS: Fifty-nine client-owned dogs attending the Veterinary Teaching Hospital. METHODS: Analytical evaluation of 100 blood samples was performed for accuracy and 23 for precision (glucose 29-579 mg/dL) following ISO recommendations. A PBGM was considered accurate if 95% of the measurements were within ±15 mg/dL from the reference when glucose was <100 mg/dL and within ±15% when it was ≥100 mg/dL, and if 99% of them were within zones A and B in error grid analysis (EG). A hexokinase-based analyzer was used as reference. Ninety samples were assessed for hematocrit interferences. RESULTS: Accuracy requirements were not fulfilled by any PBGM in WB (74% of measurements within the limits for the most accurate) and by 1 only in plasma. However, the EG analysis in WB was passed by 6 PBGM and by all in plasma. The most accurate were also the most precise, with coefficients of variation <5% in WB and <3% in plasma. Hematocrit correlated with bias against the reference method in 4 PBGM (r = -0.243 - [-0.371]; P < .021). CONCLUSIONS AND CLINICAL IMPORTANCE: This disparity among PBGM suggests that meters approved for humans need to be evaluated before use in other species.


Assuntos
Glicemia/análise , Cães/sangue , Monitorização Fisiológica/veterinária , Animais , Doenças do Cão/sangue , Monitorização Fisiológica/instrumentação , Monitorização Fisiológica/normas , Padrões de Referência , Reprodutibilidade dos Testes
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