Evaluating three commonly used growth media for assessing fumonisin analogues FB1, FB2 and FB3 production by nine Fusarium verticillioides isolates.

Maize is most often infected by the fumonisin-producing Fusarium verticillioides. Total fumonisins of natural infected grain is made up of FB1, FB2 and FB3 with FB1 occurring naturally at higher levels. A maize plant can be infected with more than one F. verticillioides isolate, and finding a reliable method to elucidate the toxigenic potential of these isolates is important to extrapolate the possible fumonisin risk to consumers of grain. It is not clear whether F. verticillioides produces similar fumonisin levels, as well as fumonisin analogue ratios, across media. In this study, nine F. verticillioides isolates were subjected to three methods of fumonisin testing using liquid media, maize patties and a field trial (silk inoculation of grain) in Potchefstroom, South Africa. Spore concentrations of 1 × 106 conidia ml-1 of each isolate were used to inoculate the different media and levels fumonisin analogues were measured using HPLC. Fumonisin production per isolate was highly variable and was influenced by the two-way interaction of F. verticillioides isolate × growth media. Total fumonisins produced in the liquid medium ranged from 0 to 21.3 ppm, on maize patties fumonisins they ranged from 0 to 21.5 ppm, and in the silk inoculation technique they ranged from 0 to 15.5 ppm. The fumonisin analogue FB1 occurred at higher levels followed by FB3 in both in vitro studies. In the silk inoculation technique, fumonisin analogue FB2 was the second highest occurring analogue after FB1. Isolate GCI 282 produced higher FB2 and FB3 levels than FB1 in the patties and grain, respectively. In order not to miscalculate the fumonisin and analogue ratio levels per F. verticillioides isolate, the growth medium will have to be optimised for each isolate and more than one growth medium used.

Multi-environment Evaluation of Maize Inbred Lines for Resistance to Fusarium Ear Rot and Fumonisins.

Fusarium verticillioides causes Fusarium ear rot (FER) of maize and produces fumonisins, which affects grain quality. Host-plant resistance can reduce both FER and fumonisins in maize. In this study, 18 maize inbred lines were evaluated for resistance to F. verticillioides and fumonisin accumulation at five localities in South Africa. Additive main effects and multiplicative interaction analyses revealed significant environment × genotype interactions, with inbred lines CML 390, US 2540W, RO 424W, and VO 617y-2 consistently exhibiting low FER severity (≤5.4%), fungal target DNA (≤0.1 ng µl-1), and fumonisin levels (≤5.6 ppm). Genotype main effect and genotype × environment biplots showed that inbred lines CML 390, US 2540W, and RO 424W were most resistant to FER, fungal colonization, and fumonisin accumulation, respectively, while inbred line RO 424W was most stable in its resistance response over environments. These inbred lines also demonstrated broad adaptability by consistently exhibiting resistance to FER, fungal colonization, and fumonisins across localities. The identified lines could serve as valuable sources of resistance against F. verticillioides and its fumonisins in local breeding programs.

Resistance in Maize Inbred Lines to Fusarium verticillioides and Fumonisin Accumulation in South Africa.

Fusarium ear rot of maize, caused by Fusarium verticillioides, is an important disease affecting maize production worldwide. Apart from reducing yield and grain quality, F. verticillioides produces fumonisins which have been associated with mycotoxicoses of animals and humans. Currently, no maize breeding lines are known with resistance to F. verticillioides in South Africa. The objective of this study, therefore, was to evaluate 24 genetically diverse maize inbred lines as potential sources of resistance to Fusarium ear rot and fumonisin accumulation in field trials at Potchefstroom and Vaalharts in South Africa. After artificial silk channel inoculation with F. verticillioides, Fusarium ear rot development was determined at harvest and fumonisins B1, B2, and B3 quantified. A significant inbred line by location effect was observed for Fusarium ear rot severity (P ≤ 0.001), although certain lines proved to be consistently resistant across both locations. The individual inbred lines also differed considerably in fumonisin accumulation between Potchefstroom and Vaalharts, with differentiation between susceptible and potentially resistant inbred lines only being possible at Vaalharts. A greenhouse inoculation trial was then also performed on a subset of potentially resistant and highly susceptible lines. The inbred lines CML 390, CML 444, CML 182, VO 617Y-2, and RO 549 W consistently showed a low Fusarium ear rot (<5%) incidence at both Potchefstroom and Vaalharts and in the greenhouse. Two of these inbred lines, CML 390 and CML 444, accumulated fumonisin levels <5 mg kg-1. These lines could potentially act as sources of resistance for use within a maize breeding program.

Incidence of Stenocarpella maydis Ear Rot of Corn Under Crop Rotation Systems.

The efficacy of crop rotation in reducing corn ear rot caused by Stenocarpella maydis in reduced and conventional tillage systems was determined over five and four seasons, respectively, at two sites in South Africa. Stenocarpella ear rot and S. maydis was isolated from kernels more frequently in monoculture corn and crop rotation where corn was planted for two consecutive seasons than where monoculture corn was interrupted by a rotation crop. Surface stubble mass, and consequently inoculum pressure, were affected similarly by crop rotation. Positive linear relationships were recorded between Stenocarpella ear rot incidence, surface stubble mass, and pycnidial counts. Wheat, soybean, and peanut were the most effective, and sunflower the least effective, rotation crops for reducing S. maydis ear rot.

Incidence of Ear Rot Pathogens Under Alternating Corn Tillage Practices.

The efficacy of periodic plowing in reduced-tillage fields in reducing corn ear rot caused by Stenocarpella maydis, Fusarium moniliforme, F. subglutinans, and F. graminearum was determined over three seasons at Bloekomspruit, South Africa. A positive linear relationship was recorded for Stenocarpella ear rot incidence and surface stubble mass. Moldboard plow plots consistently had lower stubble mass and Stenocarpella ear rot incidence than did reduced tillage practices. A cross-moldboard plow applied after one, two, and three seasons of reduced tillage reduced stubble mass and Stenocarpella ear rot incidence in the respective season only. Stenocarpella ear rot incidence increased during the subsequent season in which the original tillage practices were again applied. Alternating tillage practices would therefore not reduce Stenocarpella ear rot in the long term. Reduced disease incidence can only be achieved by moldboard plowing during each season. Alternating tillage practices had no effect on ear rots caused by Fusarium spp. during all seasons.

Use of Weather Variables to Quantify Sorghum Ergot Potential in South Africa.

Quantification of resistance to ergot requires that the observed ergot severity within a sorghum line be compared with expected ergot severity (ergot potential) to compensate for differences in environmental favorability for the disease among flowering dates and seasons. The ergot potential required to induce the onset of disease is referred to as the ergot breakdown point of that line. In earlier studies, the ergot potential of a specific flowering date was defined as the mean ergot severity in all sorghum heads over all lines in the nursery which commenced flowering on that date in a genetically broad-based sorghum nursery. In this study, results of field trials enabled accurate prediction of ergot potential by using a multiple regression analysis which included three weather variables-namely, pre-flowering minimum temperature (mean of days 23 to 27 pre-flowering), mean daily maximum temperature, and mean daily maximum relative humidity (mean of days 1 to 5 post-flowering; R2 = 0.90; P = 0.91E-5). Evaluation of predicted and observed ergot severity in an independent data set gave an index of agreement of d = 0.94 and R2 = 0.84 (P = 0.106E-4), showing that ergot severity, assuming the presence of viable inoculum, can be accurately predicted. Low pre-flowering minimum temperature was associated with reduced pollen viability, which appeared to be the primary factor predisposing lines to ergot.