Sperm ultrastructure of Pycnoderes incurvus (Hemiptera: Miridae).

The sperm morphology can provide helpful information about sexual selection, phylogeny, and the evolutionary history of a given animal group. However, there is limited or no knowledge of many taxa, especially those belonging to insects, a vast and highly diverse group. An example is the Miridae, or plant bugs, which belong to the infraorder Cimicomorpha (Heteroptera), where only three out of 17 families have published data on their sperm morphology. Here we described the Miridae sperm structure by analyzing Pycnoderes incurvus sperm under light and transmission electron microscopy. In this species, the spermatozoa were as long and slender as those of most insects. However, the anterior-most region was twisted, a characteristic first reported for Heteroptera. The acrosome was coated with electron-dense material, most likely extra-acrosomal. The centriole adjunct was a notably long, cylindrical and compact structure connecting the nucleus to the flagellar elements, with just clove-like electron-lucent points in cross-section, also features unique to Miridae so far. The flagella exhibited an axoneme of 9 + 9 + 2 microtubules and two symmetrical mitochondrial derivatives. The latter two partially embrace the axoneme, and each exhibits two paracrystalline areas and a bridge connecting it to the axoneme; these are considered Heteroptera synapomorphies that support their monophyly. RESEARCH HIGHLIGHTS: The P. incurvus sperm showed a twisted acrosome, the first reported for Heteroptera. The centriolar adjunct is a sole structure linking the nucleus and flagellum. The flagella presented the synapomorphies supporting Heteroptera monophyly.

The reproductive system of Ceracis cornifer (Mellié) and first description of sperm structure in a minute tree-fungus beetle (Tenebrionoidea: Ciidae).

Studies on the spermatogenesis of Tenebrionidae beetles (Tenebrionoidea) have shown an unusual organization of spermatozoa, in which they are arranged antiparallelly within the testicular cysts. Despite such works, many taxa of Tenebrionoidea remain to be studied, including the minute tree-fungus beetles (Ciidae). Among the challenges in the study of the internal morphology of ciids is their small body size (about 2 mm or less), which makes dissections and comparisons extremely arduous. Here, we provide, for the first time, an anatomical and histological study of the reproductive system (female and male) and a description of sperm structure of Ceracis cornifer Mellié (Ciidae), under light and transmission electron microscopes. We pointed out the growth of a single oocyte at a time in females, aside of evidences toward a constant copulatory activity that can provide a continuous supply of sperm in their small spermatheca. In males, sperm have an antiparallel organization within the cysts, a condition observed so far only in members of Tenebrionoidea. Furthermore, we observed sperm dimorphism in C. cornifer. We described, for the first time, this characteristic in a Tenebrionoidea taxon, and discussed the possible relationship between sperm dimorphism and antiparallel organization of sperm.