Limb-body wall complex: Literature review and case report.

INTRODUCTION: Body wall anomalies comprise a wide range of malformations. Limb-Body wall complex (LBWC) represents the most severe presentation of this group, with life threatening malformations in practically all the cases, including craniofacial, body wall defects, and limb anomalies. There is no consensus about its etiology and folding and gastrulation defects have been involved. Also, impaired angiogenesis has been proposed as a causative process. CASE REPORT: We present the case of a masculine stillborn, product of the first pregnancy in a 15-year-old, apparently healthy mother. He was delivered at 31 weeks of gestation due to an early rupture of membranes. He presented with multiple malformations including a wide body wall defect with multiple organ herniation and meromelia of the lower right limb. DISCUSSION AND CONCLUSIONS: LBWC represents a severe and invariably fatal pathology. There are no described risk factors, nevertheless, this case presented in a teenage mother, a well-described risk factor for other body wall anomalies. Its diagnosis allows us to discriminate between other pathologies that require prenatal or postnatal specialized treatment.

Defining the Limits of Acceptable Parenthood: Reproductive Governance in Brazil.

Drawing on sources relating to the Brazilian scenario - from ethnographic research in lower-income neighorhoods to the analysis of official documents and public debates - we build on cases of forced child removals to explore the intersectional dynamics of class, race, and gender that underlie institutionalized practices of discrimination against poverty-stricken families. After first addressing the influence of recent global trends in child-protection policy, we observe how adoption procedures in Brazil have been increasingly facilitated by the resignification of rights and corresponding changes in the country's legal infrastructures. Next, asking what sort of authoritative knowledge is invoked to define a child's best interests, we reflect on the role played by biomedicine in appraising the limits of acceptable parenthood. Guided by the notion of stratified reproduction, our investigation of these political, scientific, and moral technologies suggests plausible connections between policies that condition the demand for and the supply of adoptable children.

Detection of Trypanosoma cruzi DTUs TcI and TcIV in two outbreaks of orally-transmitted Chagas disease in the Northern region of Brazil.

This study describes the laboratory investigation of two acute Chagas disease outbreaks that occurred in the riverside communities of Marimarituba and Cachoeira do Arua, in the Santarem municipality, Para State, located in the Northern region of Brazil, and occurred in March 2016 and August 2017, respectively. The generation of data regarding the diversity of Trypanosoma cruzi parasites circulating in the Amazon region is key for understanding the emergence and expansion of Chagas disease. This study aimed to identify T. cruzi Discrete Typing Units (DTUs) involved in two outbreaks of acute Chagas disease (ACD) directly from the patient's biological sample. Nested and multiplex PCR targeting the 24Sα (rRNA) and mini-exon genes, respectively, were used to identify T. cruzi DTU in blood samples from patients diagnosed with ACD. The samples with positive cPCR were submitted for analysis for T. cruzi DTUs, which included 13 samples from the patients with ACD by oral transmission and two samples collected from two newborns of two women with ACD, from Marimarituba and Cachoeira do Arua. The samples were classified as T. cruzi TcIV, from Marimarituba's outbreak, and T. cruzi TcI, from Cachoeira do Arua's outbreak. The molecular identification of T. cruzi may increase understanding of the role of this parasite in Chagas disease's emergence within the Amazon region, contributing to the improvement of the management of this important, but also neglected, disease.

Comparative Analysis of Laparoscopic Gastrectomy Versus Laparoscopic-Assisted Gastrectomy: Postoperative Complications.

The evolution of laparoscopic surgery in gastric cancer has advanced significantly, with benefits over open surgery initially demonstrated in early gastric cancer and later in advanced stages. This study aims to evaluate postoperative complications, surgical outcomes, and anastomosis safety by comparing laparoscopic gastrectomy and laparoscopic-assisted gastrectomy. This retrospective, observational, analytical study included patients diagnosed with gastric cancer who underwent laparoscopic gastrectomy at a university hospital from January 2006 to February 2018. Patients were initially divided into two groups based on the type of anastomosis: laparoscopic gastrectomy (intracorporeal anastomosis) and laparoscopic-assisted gastrectomy (extracorporeal anastomosis). Further secondary analysis was done with subgroups based on the type of gastrectomy and anastomosis performed. A total of 139 patients were analyzed, showing significant differences in postoperative complications between the two surgical approaches. The laparoscopic-assisted group exhibited a higher rate of complications. The laparoscopic approach (with intracorporeal anastomosis) was found to have a lower risk of complications and morbidity/mortality compared to the laparoscopic-assisted approach. Laparoscopic gastrectomy with intracorporeal anastomosis resulted in lower morbidity and mortality than laparoscopic-assisted gastrectomy. The technique of partial gastrectomy with intracorporeal anastomosis was associated with the lowest rate of postoperative complications.

Detection of Trypanosoma cruzi DTUs TcI and TcIV in two outbreaks of orally-transmitted Chagas disease in the Northern region of Brazil

ABSTRACT This study describes the laboratory investigation of two acute Chagas disease outbreaks that occurred in the riverside communities of Marimarituba and Cachoeira do Arua, in the Santarem municipality, Para State, located in the Northern region of Brazil, and occurred in March 2016 and August 2017, respectively. The generation of data regarding the diversity of Trypanosoma cruzi parasites circulating in the Amazon region is key for understanding the emergence and expansion of Chagas disease. This study aimed to identify T. cruzi Discrete Typing Units (DTUs) involved in two outbreaks of acute Chagas disease (ACD) directly from the patient's biological sample. Nested and multiplex PCR targeting the 24Sα (rRNA) and mini-exon genes, respectively, were used to identify T. cruzi DTU in blood samples from patients diagnosed with ACD. The samples with positive cPCR were submitted for analysis for T. cruzi DTUs, which included 13 samples from the patients with ACD by oral transmission and two samples collected from two newborns of two women with ACD, from Marimarituba and Cachoeira do Arua. The samples were classified as T. cruzi TcIV, from Marimarituba's outbreak, and T. cruzi TcI, from Cachoeira do Arua's outbreak. The molecular identification of T. cruzi may increase understanding of the role of this parasite in Chagas disease's emergence within the Amazon region, contributing to the improvement of the management of this important, but also neglected, disease.

Evaluation of the Sensititre YeastOne and Etest in Comparison with CLSI M38-A2 for Antifungal Susceptibility Testing of Three Azoles, Amphotericin B, Caspofungin, and Anidulafungin, against Aspergillusfumigatus and Other Species, Using New Clinical Breakpoints and Epidemiological Cutoff Values.

Aspergillosis is an invasive fungal disease associated with high mortality. Antifungal susceptibility testing (AFST) is receiving increasing consideration for managing patients, as well as for surveilling emerging drug resistance, despite having time-consuming and technically complex reference methodologies. The Sensititre YeastOne (SYO) and Etest methods are widely utilized for yeasts but have not been extensively evaluated for Aspergillus isolates. We obtained Posaconazole (POS), Voriconazole (VCZ), Itraconazole (ITC), Amphotericin B (AMB), Caspofungin (CAS), and Anidulafungin (AND) minimum inhibitory concentrations (MICs) for both the Etest (n = 330) and SYO (n = 339) methods for 106 sequenced clinical strains. For 84 A. fumigatus, we analyzed the performance of both commercial methods in comparison with the CLSI-AFST, using available cutoff values. An excellent correlation could be demonstrated for Etest-AMB and Etest-VCZ (p < 0.01). SYO-MICs of AMB, VCZ, and POS resulted in excellent essential agreement (>93%), and >80% for AMB, VCZ, and ITC Etest-MICs. High categoric agreement was found for AMB, ITC, and CAS Etest-MICs (>85%) and AMB SYO-MICs (>90%). The considerable number of major/very major errors found using Etest and SYO, possibly related to the proposed cutoffs and associated with the less time-consuming processes, support the need for the improvement of commercial methods for Aspergillus strains.

Brain injury in COVID-19 is associated with dysregulated innate and adaptive immune responses.

COVID-19 is associated with neurological complications including stroke, delirium and encephalitis. Furthermore, a post-viral syndrome dominated by neuropsychiatric symptoms is common, and is seemingly unrelated to COVID-19 severity. The true frequency and underlying mechanisms of neurological injury are unknown, but exaggerated host inflammatory responses appear to be a key driver of COVID-19 severity. We investigated the dynamics of, and relationship between, serum markers of brain injury [neurofilament light (NfL), glial fibrillary acidic protein (GFAP) and total tau] and markers of dysregulated host response (autoantibody production and cytokine profiles) in 175 patients admitted with COVID-19 and 45 patients with influenza. During hospitalization, sera from patients with COVID-19 demonstrated elevations of NfL and GFAP in a severity-dependent manner, with evidence of ongoing active brain injury at follow-up 4 months later. These biomarkers were associated with elevations of pro-inflammatory cytokines and the presence of autoantibodies to a large number of different antigens. Autoantibodies were commonly seen against lung surfactant proteins but also brain proteins such as myelin associated glycoprotein. Commensurate findings were seen in the influenza cohort. A distinct process characterized by elevation of serum total tau was seen in patients at follow-up, which appeared to be independent of initial disease severity and was not associated with dysregulated immune responses unlike NfL and GFAP. These results demonstrate that brain injury is a common consequence of both COVID-19 and influenza, and is therefore likely to be a feature of severe viral infection more broadly. The brain injury occurs in the context of dysregulation of both innate and adaptive immune responses, with no single pathogenic mechanism clearly responsible.

Digital biomarker 2D:4D as a predictor of cancer: A systematic review.

BACKGROUND: The digital ratio between the second and fourth digits of the hands, known as 2D:4D, is sexually dimorphic. It has been suggested that a low 2D:4D indicates high exposure to prenatal testosterone and low estrogen exposure while a high 2D:4D indicates the inverse. The 2D:4D may be predictive of cancer susceptibility, and this may be particularly true in cancers that show differences between sexes in their occurrence. AIM: To conduct a systematic review of published epidemiological literature examining the association between 2D:4D and cancer. METHOD: This review was carried out according to criteria recommended for the systematic review of Statement PRISMA. We enrolled 25 papers involving eleven cancer topographies with 4,569 cases and 19,416 controls from Europe, America, Asia and Australia. RESULTS: We noticed variations and discrepancies in the results of the association between 2D:4D and cancer among the studies, either in those that had evaluated the same or different types of cancer, or in the same or distinct lateralities. However, a high 2D:4D was considered a likely predictor of cancer risk in 11 of 25 studies, a low 2D:4D was suggested as a predictor of risk in eight papers, and five of the twenty-five studies did not demonstrate any association. CONCLUSIONS: Although this biomarker has the advantage of being easy to measure, it is noted that its relationship with sex hormone levels at specific stages of life has not yet been well quantified, and it has still been questioned. Hence, it is suggested that those findings from studies involving 2D:4D as a proxy for foetal hormone exposure should be interpreted with caution, as well as those studies which claim its association with cancer. Thus, this review shows the need for a greater number of epidemiological studies using more homogeneous methodology and techniques to better investigate the strength of the findings.

Poor Sleep quality and health-related quality of life impact in adolescents with and without chronic immunosuppressive conditions during COVID-19 quarantine.

OBJECTIVE: To assess the possible factors that influence sleep quality in adolescents with and without chronic immunosuppressive conditions quarantined during the coronavirus disease 2019 (COVID-19) pandemic. METHODS: This cross-sectional study included 305 adolescents with chronic immunocompromised conditions and 82 healthy adolescents. Online surveys were completed, which included questions on socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and the following validated questionnaires: the Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0), and Pediatric Outcome Data Collection Instrument (PODCI). RESULTS: The median current age [14 (10-18) vs. 15 (10-18) years, p=0.847] and frequency of female sex (62% vs. 58%, p=0.571) were similar in adolescents with chronic conditions compared with healthy adolescents. The frequency of poor sleep quality was similar in both groups (38% vs. 48%, p=0.118). Logistic regression analysis, including both healthy adolescents and adolescents with chronic conditions (n=387), demonstrated that self-reported increase in screen time (odds ratio [OR] 3.0; 95% confidence interval [CI] 1.3-6.8; p=0.008) and intrafamilial violence report (OR 2.1; 95% CI 1.2-3.5; p=0.008) were independently associated with poor sleep quality in these adolescents. However, the PODCI global function score was associated with a lower OR for poor sleep quality (OR 0.97; 95% CI 0.94-0.99; p=0.001). Further logistic regression, including only adolescents with chronic conditions (n=305), demonstrated that self-reported increase in screen time (OR 3.1; 95% CI 1.4-6.8; p=0.006) and intrafamilial violence report (OR 2.0; 95% CI 1.2-3.4; p=0.011) remained independently associated with poor quality of sleep, whereas a lower PODCI global function score was associated with a lower OR for sleep quality (OR 0.96; 95% CI 0.94-0.98; p<0.001). CONCLUSION: Self-reported increases in screen time and intrafamilial violence report impacted sleep quality in both healthy adolescents and those with chronic conditions. Decreased health-related quality of life was observed in adolescents with poor sleep quality.

Monocyte-Derived Dendritic Cells Can Revert In Vitro Antigen-Specific Cellular Anergy in Active Human Paracoccidioidomycosis.

We investigated the in vitro effects of two Paracoccidioides brasiliensis antigens on monocyte-derived dendritic cells (moDCs) from patients with paracoccidioidomycosis (PCM). MoDCs from patients with active or treated PCM and non-PCM subjects were generated, stimulated with TNF-α, and P. brasiliensis antigens, 43 kDa glycoprotein (gp43) and cell-free antigen (CFA), and analyzed by flow cytometry and enzyme-linked immunosorbent assays (ELISA). Our data revealed that patients with PCM had a high frequency of HLA-DR+ cells, but the treated group had more CD86+ cells with increased IL-12p40. Patients with active PCM had more CD80+ moDCs, and as a novel finding, large amounts of chemokine (C-C motif) ligand 18 (CCL18) in the supernatants from their in vitro moDC cultures. Both gp43- and CFA-stimulated moDCs from the patients with PCM successfully reverted the in vitro antigen-specific anergy, inducing a proliferative response. However, CFA-stimulated moDCs led to higher lymphoproliferation, with increased IFN-γ and TNF-α in the cells from the patients with active PCM compared with gp43. These original results combined with constant IL-10 and increased IL-12p40 levels suggest that a more complex antigen, such as CFA, may be a better inducer of the protective Th1 immune response than purified gp43 is, and a suitable target for future studies on anti-P. brasiliensis dendritic cell (DC)-based vaccines.

Clinician prescription of lipid-lowering drugs and achievement of treatment goals in patients with newly diagnosed type 2 diabetes mellitus.

INTRODUCTION: Lipid control is essential in type 2 diabetes mellitus (T2DM). The aim of this study is to investigate factors associated with lipid therapy adherence and achievement of goals in real-life setting among patients with recently diagnosed T2DM. RESEARCH DESIGN AND METHODS: This is a longitudinal analysis in a center of comprehensive care for patients with diabetes. We include patients with T2DM, <5 years of diagnosis, without disabling complications (eg, amputation, myocardial infarct, stroke, proliferative retinopathy, glomerular filtration rate <60 mL/min/m2) and completed 2-year follow-up. The comprehensive diabetes care model includes 9 interventions in 4 initial visits and annual evaluations. Endocrinologists follow the clinic's guideline and adapt therapy to reach risk-based treatment goal. The main outcome measures were the proportion of patients meeting low-density lipoprotein cholesterol (c-LDL) (<100 mg/dL) and triglycerides (<150 mg/dL) and proportion of patients taking statin, fibrate or combination at baseline, 3 months and annual evaluations. RESULTS: We included 288 consecutive patients (54±9 years, 53.8% women), time since T2DM diagnosis 1 (0-5) year. Baseline, 10.8% patients were receiving statin therapy (46.5% moderate-intensity therapy and 4.6% high-intensity therapy), 8.3% fibrates and 4.2% combined treatment. The proportion of patients with combined treatment increased to 41.6% at 3 months, decreased to 20.8% at 1 year and increased to 38.9% at 2 years of evaluation. Patients receiving treatment met LDL and triglycerides goals at 3 months (17% vs 59.7%, relative ratio (RR)=0.89, 95% CI 0.71 to 1.12), at 1 year (17% vs 26.7%, RR=0.62, 95% CI 0.41 to 0.95) and at 2 years (17% vs 29.9%, RR=0.63, 95% CI 0.43 to 0.93). Main reasons for medication suspension: patient considered treatment was not important (37.5%) and other physician suspended treatment (31.3%). CONCLUSION: 88.2% of patients with T2DM required lipid-lowering drugs. Education for patients and physicians is critical to achieve and maintain diabetes goals. TRIAL REGISTRATION NUMBER: NCT02836808.

Poor Sleep quality and health-related quality of life impact in adolescents with and without chronic immunosuppressive conditions during COVID-19 quarantine

OBJECTIVE: To assess the possible factors that influence sleep quality in adolescents with and without chronic immunosuppressive conditions quarantined during the coronavirus disease 2019 (COVID-19) pandemic. METHODS: This cross-sectional study included 305 adolescents with chronic immunocompromised conditions and 82 healthy adolescents. Online surveys were completed, which included questions on socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and the following validated questionnaires: the Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0), and Pediatric Outcome Data Collection Instrument (PODCI). RESULTS: The median current age [14 (10-18) vs. 15 (10-18) years, p=0.847] and frequency of female sex (62% vs. 58%, p=0.571) were similar in adolescents with chronic conditions compared with healthy adolescents. The frequency of poor sleep quality was similar in both groups (38% vs. 48%, p=0.118). Logistic regression analysis, including both healthy adolescents and adolescents with chronic conditions (n=387), demonstrated that self-reported increase in screen time (odds ratio [OR] 3.0; 95% confidence interval [CI] 1.3-6.8; p=0.008) and intrafamilial violence report (OR 2.1; 95% CI 1.2-3.5; p=0.008) were independently associated with poor sleep quality in these adolescents. However, the PODCI global function score was associated with a lower OR for poor sleep quality (OR 0.97; 95% CI 0.94-0.99; p=0.001). Further logistic regression, including only adolescents with chronic conditions (n=305), demonstrated that self-reported increase in screen time (OR 3.1; 95% CI 1.4-6.8; p=0.006) and intrafamilial violence report (OR 2.0; 95% CI 1.2-3.4; p=0.011) remained independently associated with poor quality of sleep, whereas a lower PODCI global function score was associated with a lower OR for sleep quality (OR 0.96; 95% CI 0.94-0.98; p<0.001). CONCLUSION: Self-reported increases in screen time and intrafamilial violence report impacted sleep quality in both healthy adolescents and those with chronic conditions. Decreased health-related quality of life was observed in adolescents with poor sleep quality.

Educação, Saúde Pública e Teoria da Comunicação: o curso de pósgraduação em Comunicação e Saúde da ESP-MG / Education, Public Health and Communication Theory: the specialization course in Communication and Health of ESP-MG / Educación, Salud Pública y Teoría de la Comunicación: el curso de especialización en Comunicación y Salud de la ESP-MG

A interface entre a comunicação e a saúde é construída coletivamente, envolvendo uma miríade de interlocutores em contextos sociais determinados, com o objetivo final de tornar efetivos os direitos à comunicação e à saúde, previstos na Constituição Federal de 1988. Para promover este encontro, bem como capacitar profissionais de ambas as áreas, a Escola de Saúde Pública de Minas Gerais (ESP-MG) oferece o curso de Pós-graduação em Comunicação e Saúde, no qual a disciplina Teorias da Comunicação tem destaque, uma vez que é primordial conhecer os fundamentos da comunicação humana e a dinâmica da Comunicação Social, com ênfase no campo teórico-prático conhecido como Comunicação e Saúde (CeS). Este artigo relata a experiência do curso a partir da disciplina Teorias da Comunicação, de modo a situá-la como conhecimento essencial não somente para os comunicadores, mas também para os profissionais de saúde.

The interface between communication and health is built collectively, involving a myriad of interlocutors in determined social contexts, with the ultimate goal of making the communication and health rights established in the 1988 Federal Constitution effective. In order to promote this exchange, as well as training professionals from both areas, the School of Public Health of Minas Gerais (SP-MG) offers the postgraduate course in Communication and Health, in which the Theories of Communication discipline is highlighted, since it is essential to know the fundamentals of human communication and the dynamics of Social Communication, with emphasis on the theoretical-practical field known as Communication and Health (CES). This article reports the experience of the course from the Theories of Communication subject, in order to situate it as essential knowledge not only for communicators, but also for health professionals.

La interfaz entre la comunicación y la salud se construye colectivamente, involucrando una miríada de interlocutores en contextos sociales determinados, con el objetivo final de hacer efectivos los derechos a la comunicación ya las saludes previstas en la Constitución Federal de 1988. Para promover este encuentro, como la capacitación de profesionales de ambas áreas, la Escuela de Salud Pública de Minas Gerais (ESP-MG) ofrece el curso de Postgrado en Comunicación y Salud, en el cual la disciplina Teorías de la Comunicación tiene destaque, ya que es fundamental conocer los fundamentos de la comunicación humana y la dinámica de la Comunicación Social, con énfasis en el campo teórico-práctico conocido como Comunicación y Salud (CeS). Este artículo relata la experiencia del curso a partir de la disciplina Teorías de la Comunicación, para situarla como conocimiento esencial no sólo para los comunicadores, sino también para los profesionales de la salud.

A Real Time PCR strategy for the detection and quantification of Candida albicans in human blood.

Candidemia is a significant cause of bloodstream infections (BSI) in nosocomial settings. The identification of species can potentially improve the quality of care and decrease human mortality. Quantitative PCR (qPCR) was evaluated for Candida albicans detection using culture suspensions containing C. albicans , spiked human blood, the cloned qPCR target fragment (ITS2 region) and the results of these assays were compared. The assays showed a good detection limit: C. albicans DNA extracted from yeast (sensitivity 0.2 CFU/µL), spiked human blood (sensitivity 10 CFU/mL), and cloned fragment of ITS2 region (sensitivity 20 target copies/µL). The efficiency of ITS2 fragment-qPCR ranged from 89.67 to 97.07, and the linearity (R2) of the standard curve ranged from 0.992 to 0.999. The results showed that this ITS2-qPCR has a great potential as a molecular prototype model for the development of a test to be applied in clinical practice, greatly reducing the time of candidemia diagnosis, which is extremely important in this clinical setting.

DNA and the displacement of certainties in brazilian family law / DNA e o deslocamento de certezas no direito de família brasileiro / ADN y el desplazamiento de verdades en la ley de la familia brasileña

Abstract Inquiring into the impact of DNA technology on Brazilian family law,, through the consultation of legislation, jurisprudence and specific legal briefs concerning paternity disputes, we map out trends over the past thirty years. We show how, after a moment of original skepticism, genetic evidence appears to dominate the legal scene, rendering personal testimony irrelevant. However, with growing concern about men who use a negative test result to disclaim their paternal status, this testimony is once again needed to clarify whether or not the man originally believed the child was his blood-related offspring. Finally, we look at a recent period, showing how reactions against the "real biological truth" have spawned a new juridical category-socio-affective paternity-that, spreading well beyond paternity tests, is altering some basic tenets of family law.

Resumo Enfocando o impacto da tecnologia do DNA no direito de família brasileiro, pela análise de leis, jurisprudência e processos particulares lidando com disputas de paternidade, mapeamos tendências de mudança ao longo dos últimos trinta anos. Mostramos como, após um momento de ceticismo inicial, a evidência genética parece dominar o cenário legal, tornando os depoimentos pessoais irrelevantes. Entretanto, com a atenção crescente dirigida para homens que usam um resultado negativo para refutar seu status paterno, a importância de depoimentos volta para esclarecer se o pai registral originalmente acreditava ou não que tinha um vínculo consanguíneo com seu filho. Finalmente, olhamos para um período recente, rastreando como reações contra a "verdade real" da biologia geram uma nova categoria jurídica - paternidade socioafetiva - que, estendendo-se muito além dos testes de paternidade, está alterando alguns princípios básicos do direito da família.

Resumen Centrándonos en el impacto de la tecnología del ADN en el derecho de familia brasileño, analizando las leyes, la jurisprudencia y las demandas particulares relacionadas con disputas de paternidad, trazando tendencias cambiantes en los últimos treinta años. Mostramos como, después de un momento de escepticismo inicial, la evidencia genética parece dominar el panorama legal, haciendo irrelevantes los testimonios personales. Sin embargo, con la atención cada vez mayor dirigida a los hombres que usan un resultado negativo para refutar su paternidad, la importancia de los testimonios vuelve a aclarar si el padre de registro originalmente creía o no, tener un vínculo consanguíneo con su hijo. Finalmente, observamos un período reciente, rastreando como las reacciones contra la "verdad real" de la biología, generan una nueva categoría legal, paternidad socioafectiva, que, más allá de las pruebas de paternidad, está alterando algunos principios básicos del derecho de familia.

Alta frequência dos fatores de risco à oclusão dentária entre escolares no município de Petrópolis: um estudo transversal / High frequency of damaging factors to dental occlusion among schoolchildren in the municipality of Petrópolis: a cross sectional study

INTRODUÇÃO: A má oclusão dentária tem origem multifatorial, logo é difícil definir estratégicas específicas de como preveni-la. A respiração bucal e certos hábitos de sucção, se persistir por mais de 36 meses, podem influenciar de forma negativa. OBJETIVO: Identificar fatores de risco à oclusão dentária como: respiração bucal e hábitos de sucção em escolares de 8 a 10 anos de idade em Petrópolis, RJ. MÉTODOS: Estudo observacional, transversal, de caráter descritivo. Incluídos escolares de ambos os sexos, 8 a 10 anos, matriculados em sete escolas municipais de Petrópolis. Distribuído aos escolares o protocolo para a identificação da criança respiradora bucal e o questionário sobre hábitos de sucção (mamadeira, chupeta e dedo). RESULTADOS: Foram avaliados 377 protocolos para a identificação de criança respiradora bucal e 377 questionários sobre hábitos de sucção. Nos protocolos foram referidos sintomas como: dormir de boca aberta em 193 (51,2%), babar no travesseiro em 172 (45,6%), roncos em 131 (34,7%) e obstrução nasal diária em 118 (31,2%). Ao aplicar os critérios de Abreu, constatou-se uma frequência de 243 (64%) respiradores bucais e 134 (36%) respiradores nasais. Ao avaliar os 377 questionários sobre os hábitos de sucção encontraram-se: 276 (73%) crianças com hábitos de sucção e 101 (27%) sem tais hábitos. Dos 276 escolares que tiveram hábitos de sucção, houve persistência destes hábitos acima de três anos e 11 meses em 149 crianças (54%). CONCLUSÃO: Encontrada uma alta frequência de respiradores bucais e de crianças com hábitos de sucção.

INTRODUCTION: Dental malocclusion has a multifactorial origin, so it is difficult to define specific strategies for preventing it. Mouth breathing and certain sucking habits, if they persist for more than 36 months, can have a negative influence. OBJECTIVE: To identify potentially damaging factors to dental occlusion like mouth breathing and sucking habits in children aged 8 to 10 years old in Petrópolis, RJ. METHODS: Cross-sectional, observational, descriptive study. Both male and female children between the ages of 8 to 10 years, enrolled in seven public schools of Petrópolis, were included. The identification protocol for mouth breathing child and the questionnaire on sucking habits (feeding bottle, pacifier and finger sucking) were applied to the students. RESULTS: A total of 377 protocols for identification of mouth breathing children and 377 questionnaires for suction habits were evaluated. The following symptoms were reported: open mouth sleeping in 193 (51.2%), drooling on the pillow in 172 (45.6%), snoring in 131 (34.7%) and daily nasal obstruction in 118 (31,2%). After application of Abreu criteria, a frequency of 243 (64%) mouth breathers and 134 (36%) nasal breathers was observed. Evaluation of the questionnaires about sucking habits demontrated 276 (73%) children with sucking habits and 101 (27%) without such habits. Of the 276 schoolchildren with sucking habits, those habits persisted for more than 3 years and 11 months in 149 children (54%). CONCLUSION: A high frequency of mouth breathing and sucking habits children were found.

Avaliação do Seguimento Clínico de Citopatologia Oncótica em Mulheres na Atenção Primária à Saúde / Evaluation of Clinical Follow of Cytopathology Oncotic in Women in Primary Health Care

Objetivo: Este estudo avaliou o seguimento clínico e terapêutico da citopatologia oncótica em mulheres na Atenção Primária à Saúde (APS). Métodos: Trata-se de um estudo exploratóriodescritivo, de abordagem quantitativa, realizado na Cidade de Santa Cruz/RN, Brasil, o qual se utilizou de uma amostra de 150 mulheres. A pesquisa foi aprovada pelo Comitê de Ética em Pesquisa da Universidade Federal do Rio Grande do Norte, sob número de parecer 313.430. Resultados: Observou-se que, no tocante ao resultado do exame preventivo, 66% da amostra referiu ter apresentado Cândida albicans, seguido por 28,7% que apresentaram positividade de Gardnerella vaginallis, e que 4,7% tiveram resultados positivos para HPV e NIC I. Conclusões: Observou-se a continuidade da assistência para mulheres que apresentaram NIC I e HPV, no entanto, espera-se que os profissionais de saúde estimulem o acompanhamento da mulher na rede básica de saúde e, consequentemente, fortaleçam o vínculo entre o usuário-serviço, de modo que favoreça a assistência.

Objective: This study aims to evaluate the Clinical and therapeutic follow-up of oncotic cytopathology in women in Primary Health Care (PHC). Methods: This was an exploratory descriptive study with a quantitative approach, in the city of Santa Cruz / RN, Brazil, which used a sample of 150 women. The study was approved by the Ethics Committee in Research of the Federal University of Rio Grande do Norte, under number 313 430. Results: It was observed that, in relation to the result of the screening test, 66% of the sample reported having presented Candida albicans, followed by 28.7% who were positive for Gardnerella vaginallis, and 4.7% were positive for HPV and CIN I. Conclusions: it was observed the continuity of care for women with CIN I and HPV, however, it is expected that health professionals encourage the monitoring of women in primary health care network and thus strengthen the bond between the user-service, so that it can favor assistance. (AU)