RESUMO
Leishmania parasites cause a broad spectrum of clinical disease. Here we report the sequencing of the genomes of two species of Leishmania: Leishmania infantum and Leishmania braziliensis. The comparison of these sequences with the published genome of Leishmania major reveals marked conservation of synteny and identifies only approximately 200 genes with a differential distribution between the three species. L. braziliensis, contrary to Leishmania species examined so far, possesses components of a putative RNA-mediated interference pathway, telomere-associated transposable elements and spliced leader-associated SLACS retrotransposons. We show that pseudogene formation and gene loss are the principal forces shaping the different genomes. Genes that are differentially distributed between the species encode proteins implicated in host-pathogen interactions and parasite survival in the macrophage.
Assuntos
Genoma , Genômica , Leishmania/genética , Leishmaniose/parasitologia , Sequência de Aminoácidos , Animais , Humanos , Leishmania braziliensis/genética , Leishmania infantum/genética , Leishmania major/genética , Leishmaniose Cutânea/parasitologia , Leishmaniose Visceral/parasitologia , Dados de Sequência MolecularRESUMO
Clostridium botulinum is a heterogeneous Gram-positive species that comprises four genetically and physiologically distinct groups of bacteria that share the ability to produce botulinum neurotoxin, the most poisonous toxin known to man, and the causative agent of botulism, a severe disease of humans and animals. We report here the complete genome sequence of a representative of Group I (proteolytic) C. botulinum (strain Hall A, ATCC 3502). The genome consists of a chromosome (3,886,916 bp) and a plasmid (16,344 bp), which carry 3650 and 19 predicted genes, respectively. Consistent with the proteolytic phenotype of this strain, the genome harbors a large number of genes encoding secreted proteases and enzymes involved in uptake and metabolism of amino acids. The genome also reveals a hitherto unknown ability of C. botulinum to degrade chitin. There is a significant lack of recently acquired DNA, indicating a stable genomic content, in strong contrast to the fluid genome of Clostridium difficile, which can form longer-term relationships with its host. Overall, the genome indicates that C. botulinum is adapted to a saprophytic lifestyle both in soil and aquatic environments. This pathogen relies on its toxin to rapidly kill a wide range of prey species, and to gain access to nutrient sources, it releases a large number of extracellular enzymes to soften and destroy rotting or decayed tissues.
Assuntos
Clostridium botulinum/genética , Genoma Bacteriano , Animais , Toxinas Botulínicas/genética , Botulismo , Cromossomos Bacterianos , Clostridium botulinum/classificação , DNA Bacteriano/genética , DNA Circular/genética , Enzimas/genética , Genômica , Bactérias Gram-Positivas/genética , Humanos , Dados de Sequência Molecular , Neurotoxinas/genética , Virulência/genéticaRESUMO
Toxoplasma gondii is a globally distributed protozoan parasite that can infect virtually all warm-blooded animals and humans. Despite the existence of a sexual phase in the life cycle, T. gondii has an unusual population structure dominated by three clonal lineages that predominate in North America and Europe, (Types I, II, and III). These lineages were founded by common ancestors approximately10,000 yr ago. The recent origin and widespread distribution of the clonal lineages is attributed to the circumvention of the sexual cycle by a new mode of transmission-asexual transmission between intermediate hosts. Asexual transmission appears to be multigenic and although the specific genes mediating this trait are unknown, it is predicted that all members of the clonal lineages should share the same alleles. Genetic mapping studies suggested that chromosome Ia was unusually monomorphic compared with the rest of the genome. To investigate this further, we sequenced chromosome Ia and chromosome Ib in the Type I strain, RH, and the Type II strain, ME49. Comparative genome analyses of the two chromosomal sequences revealed that the same copy of chromosome Ia was inherited in each lineage, whereas chromosome Ib maintained the same high frequency of between-strain polymorphism as the rest of the genome. Sampling of chromosome Ia sequence in seven additional representative strains from the three clonal lineages supports a monomorphic inheritance, which is unique within the genome. Taken together, our observations implicate a specific combination of alleles on chromosome Ia in the recent origin and widespread success of the clonal lineages of T. gondii.
Assuntos
Cromossomos , Evolução Molecular , Toxoplasma/genética , Animais , Cruzamentos Genéticos , Variação Genética , Genética Populacional , Padrões de Herança , Meiose , Dados de Sequência Molecular , Recombinação Genética , Toxoplasma/classificaçãoRESUMO
We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.
Assuntos
Clostridioides difficile/genética , Clostridioides difficile/patogenicidade , Adaptação Fisiológica , Proteínas de Bactérias/genética , Sequência de Bases , Clostridioides difficile/efeitos dos fármacos , Clostridioides difficile/fisiologia , Conjugação Genética , Elementos de DNA Transponíveis/genética , DNA Bacteriano/genética , Farmacorresistência Bacteriana Múltipla/genética , Enterocolite Pseudomembranosa/etiologia , Enterocolite Pseudomembranosa/microbiologia , Trato Gastrointestinal/microbiologia , Genoma Bacteriano , Humanos , Dados de Sequência Molecular , Mosaicismo , Análise de Sequência com Séries de Oligonucleotídeos , Esporos Bacterianos/fisiologia , Virulência/genéticaRESUMO
BACKGROUND: Rhizobium leguminosarum is an alpha-proteobacterial N2-fixing symbiont of legumes that has been the subject of more than a thousand publications. Genes for the symbiotic interaction with plants are well studied, but the adaptations that allow survival and growth in the soil environment are poorly understood. We have sequenced the genome of R. leguminosarum biovar viciae strain 3841. RESULTS: The 7.75 Mb genome comprises a circular chromosome and six circular plasmids, with 61% G+C overall. All three rRNA operons and 52 tRNA genes are on the chromosome; essential protein-encoding genes are largely chromosomal, but most functional classes occur on plasmids as well. Of the 7,263 protein-encoding genes, 2,056 had orthologs in each of three related genomes (Agrobacterium tumefaciens, Sinorhizobium meliloti, and Mesorhizobium loti), and these genes were over-represented in the chromosome and had above average G+C. Most supported the rRNA-based phylogeny, confirming A. tumefaciens to be the closest among these relatives, but 347 genes were incompatible with this phylogeny; these were scattered throughout the genome but were over-represented on the plasmids. An unexpectedly large number of genes were shared by all three rhizobia but were missing from A. tumefaciens. CONCLUSION: Overall, the genome can be considered to have two main components: a 'core', which is higher in G+C, is mostly chromosomal, is shared with related organisms, and has a consistent phylogeny; and an 'accessory' component, which is sporadic in distribution, lower in G+C, and located on the plasmids and chromosomal islands. The accessory genome has a different nucleotide composition from the core despite a long history of coexistence.
Assuntos
Genoma Bacteriano , Rhizobium leguminosarum/genética , Transportadores de Cassetes de Ligação de ATP/genética , Transportadores de Cassetes de Ligação de ATP/metabolismo , Adaptação Fisiológica , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Composição de Bases , Sequência de Bases , Replicação do DNA/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Ecossistema , Evolução Molecular , Fabaceae/microbiologia , Genes Bacterianos , Fixação de Nitrogênio/genética , Filogenia , Plasmídeos/química , Plasmídeos/genética , Replicon , Rhizobium leguminosarum/crescimento & desenvolvimento , Rhizobium leguminosarum/fisiologia , Simbiose/genética , Simbiose/fisiologiaRESUMO
Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen. Its conidia production is prolific, and so human respiratory tract exposure is almost constant. A. fumigatus is isolated from human habitats and vegetable compost heaps. In immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). The interaction of A. fumigatus and other airborne fungi with the immune system is increasingly linked to severe asthma and sinusitis. Although the burden of invasive disease caused by A. fumigatus is substantial, the basic biology of the organism is mostly obscure. Here we show the complete 29.4-megabase genome sequence of the clinical isolate Af293, which consists of eight chromosomes containing 9,926 predicted genes. Microarray analysis revealed temperature-dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype. The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus.
Assuntos
Alérgenos/genética , Aspergillus fumigatus/genética , Aspergillus fumigatus/patogenicidade , Genoma Fúngico , Genômica , Hipersensibilidade/microbiologia , Aspergillus fumigatus/imunologia , Perfilação da Expressão Gênica , Regulação Fúngica da Expressão Gênica , Genes Fúngicos/genética , Dados de Sequência Molecular , Análise de Sequência com Séries de Oligonucleotídeos , Análise de Sequência de DNA , Temperatura , Virulência/genéticaRESUMO
African trypanosomes cause human sleeping sickness and livestock trypanosomiasis in sub-Saharan Africa. We present the sequence and analysis of the 11 megabase-sized chromosomes of Trypanosoma brucei. The 26-megabase genome contains 9068 predicted genes, including approximately 900 pseudogenes and approximately 1700 T. brucei-specific genes. Large subtelomeric arrays contain an archive of 806 variant surface glycoprotein (VSG) genes used by the parasite to evade the mammalian immune system. Most VSG genes are pseudogenes, which may be used to generate expressed mosaic genes by ectopic recombination. Comparisons of the cytoskeleton and endocytic trafficking systems with those of humans and other eukaryotic organisms reveal major differences. A comparison of metabolic pathways encoded by the genomes of T. brucei, T. cruzi, and Leishmania major reveals the least overall metabolic capability in T. brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified.
Assuntos
Genoma de Protozoário , Glutationa/análogos & derivados , Proteínas de Protozoários/genética , Análise de Sequência de DNA , Espermidina/análogos & derivados , Trypanosoma brucei brucei/genética , Aminoácidos/metabolismo , Animais , Variação Antigênica , Antígenos de Protozoários/química , Antígenos de Protozoários/genética , Antígenos de Protozoários/imunologia , Metabolismo dos Carboidratos , Cromossomos/genética , Citoesqueleto/química , Citoesqueleto/genética , Citoesqueleto/fisiologia , Ergosterol/biossíntese , Genes de Protozoários , Glutationa/metabolismo , Glicosilfosfatidilinositóis/biossíntese , Humanos , Metabolismo dos Lipídeos , Dados de Sequência Molecular , Transporte Proteico , Proteínas de Protozoários/química , Proteínas de Protozoários/metabolismo , Pseudogenes , Purinas/metabolismo , Pirimidinas/biossíntese , Recombinação Genética , Espermidina/metabolismo , Trypanosoma brucei brucei/química , Trypanosoma brucei brucei/imunologia , Trypanosoma brucei brucei/metabolismo , Tripanossomíase Africana/parasitologiaRESUMO
Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.
Assuntos
Genoma de Protozoário , Leishmania major/genética , Análise de Sequência de DNA , Animais , Cromatina/genética , Cromatina/metabolismo , Regulação da Expressão Gênica , Genes de Protozoários , Genes de RNAr , Glicoconjugados/biossíntese , Glicoconjugados/metabolismo , Leishmania major/química , Leishmania major/metabolismo , Leishmaniose Cutânea/parasitologia , Metabolismo dos Lipídeos , Proteínas de Membrana/biossíntese , Proteínas de Membrana/química , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Dados de Sequência Molecular , Família Multigênica , Biossíntese de Proteínas , Processamento de Proteína Pós-Traducional , Proteínas de Protozoários/biossíntese , Proteínas de Protozoários/química , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo , Processamento Pós-Transcricional do RNA , Splicing de RNA , RNA de Protozoário/genética , RNA de Protozoário/metabolismo , Transcrição GênicaRESUMO
Theileria annulata and T. parva are closely related protozoan parasites that cause lymphoproliferative diseases of cattle. We sequenced the genome of T. annulata and compared it with that of T. parva to understand the mechanisms underlying transformation and tropism. Despite high conservation of gene sequences and synteny, the analysis reveals unequally expanded gene families and species-specific genes. We also identify divergent families of putative secreted polypeptides that may reduce immune recognition, candidate regulators of host-cell transformation, and a Theileria-specific protein domain [frequently associated in Theileria (FAINT)] present in a large number of secreted proteins.
Assuntos
Genoma de Protozoário , Proteínas de Protozoários/genética , Theileria annulata/genética , Theileria parva/genética , Motivos de Aminoácidos , Animais , Bovinos , Proliferação de Células , Mapeamento Cromossômico , Cromossomos/genética , Sequência Conservada , Genes de Protozoários , Estágios do Ciclo de Vida , Metabolismo dos Lipídeos , Linfócitos/citologia , Linfócitos/parasitologia , Dados de Sequência Molecular , Família Multigênica , Filogenia , Sinais Direcionadores de Proteínas/genética , Estrutura Terciária de Proteína , Proteoma , Proteínas de Protozoários/química , Proteínas de Protozoários/fisiologia , Análise de Sequência de DNA , Especificidade da Espécie , Sintenia , Telômero/genética , Theileria annulata/crescimento & desenvolvimento , Theileria annulata/imunologia , Theileria annulata/patogenicidade , Theileria parva/crescimento & desenvolvimento , Theileria parva/imunologia , Theileria parva/patogenicidadeRESUMO
In the fungus Pneumocystis carinii, at least three gene families (PRT1, MSR, and MSG) have the potential to generate high-frequency antigenic variation, which is likely to be a strategy by which this parasitic fungus is able to prolong its survival in the rat lung. Members of these gene families are clustered at chromosome termini, a location that fosters recombination, which has been implicated in selective expression of MSG genes. To gain insight into the architecture, evolution, and regulation of these gene clusters, six telomeric segments of the genome were sequenced. Each of the segments began with one or more unique genes, after which were members of different gene families, arranged in a head-to-tail array. The three-gene repeat PRT1-MSR-MSG was common, suggesting that duplications of these repeats have contributed to expansion of all three families. However, members of a gene family in an array were no more similar to one another than to members in other arrays, indicating rapid divergence after duplication. The intergenic spacers were more conserved than the genes and contained sequence motifs also present in subtelomeres, which in other species have been implicated in gene expression and recombination. Long mononucleotide tracts were present in some MSR genes. These unstable sequences can be expected to suffer frequent frameshift mutations, providing P. carinii with another mechanism to generate antigen variation.
Assuntos
Genes Fúngicos , Pneumocystis carinii/genética , Telômero/genética , Sequência de Aminoácidos , Antígenos de Fungos , Sequência de Bases , Mapeamento Cromossômico , Cromossomos Fúngicos , Clonagem Molecular , Cosmídeos , DNA Fúngico , Evolução Molecular , Duplicação Gênica , Regulação Fúngica da Expressão Gênica , Biblioteca Gênica , Ligação Genética , Genoma Fúngico , Fases de Leitura Aberta , RNA Mensageiro/genética , Recombinação Genética , Sequências Repetitivas de Ácido Nucleico , Seleção Genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
The oomycete Phytophthora infestans causes late blight, the potato disease that precipitated the Irish famines in 1846 and 1847. It represents a reemerging threat to potato production and is one of >70 species that are arguably the most devastating pathogens of dicotyledonous plants. Nevertheless, little is known about the molecular bases of pathogenicity in these algae-like organisms or of avirulence molecules that are perceived by host defenses. Disease resistance alleles, products of which recognize corresponding avirulence molecules in the pathogen, have been introgressed into the cultivated potato from a wild species, Solanum demissum, and R1 and R3a have been identified. We used association genetics to identify Avr3a and show that it encodes a protein that is recognized in the host cytoplasm, where it triggers R3a-dependent cell death. Avr3a resides in a region of the P. infestans genome that is colinear with the locus containing avirulence gene ATR1(NdWsB) in Hyaloperonospora parasitica, an oomycete pathogen of Arabidopsis. Remarkably, distances between conserved genes in these avirulence loci were often similar, despite intervening genomic variation. We suggest that Avr3a has undergone gene duplication and that an allele evading recognition by R3a arose under positive selection.
Assuntos
Proteínas de Algas/genética , Apoptose/genética , Phytophthora/genética , Phytophthora/patogenicidade , Solanum tuberosum/microbiologia , Agrobacterium tumefaciens , Proteínas de Algas/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Biolística , Cromossomos Artificiais Bacterianos , Citoplasma/metabolismo , Primers do DNA , Duplicação Gênica , Vetores Genéticos , Proteínas de Fluorescência Verde , Funções Verossimilhança , Modelos Genéticos , Dados de Sequência Molecular , Filogenia , Potexvirus , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Análise de Sequência de DNA , Solanum tuberosum/genética , Sintenia/genética , VirulênciaRESUMO
Burkholderia pseudomallei is a recognized biothreat agent and the causative agent of melioidosis. This Gram-negative bacterium exists as a soil saprophyte in melioidosis-endemic areas of the world and accounts for 20% of community-acquired septicaemias in northeastern Thailand where half of those affected die. Here we report the complete genome of B. pseudomallei, which is composed of two chromosomes of 4.07 megabase pairs and 3.17 megabase pairs, showing significant functional partitioning of genes between them. The large chromosome encodes many of the core functions associated with central metabolism and cell growth, whereas the small chromosome carries more accessory functions associated with adaptation and survival in different niches. Genomic comparisons with closely and more distantly related bacteria revealed a greater level of gene order conservation and a greater number of orthologous genes on the large chromosome, suggesting that the two replicons have distinct evolutionary origins. A striking feature of the genome was the presence of 16 genomic islands (GIs) that together made up 6.1% of the genome. Further analysis revealed these islands to be variably present in a collection of invasive and soil isolates but entirely absent from the clonally related organism B. mallei. We propose that variable horizontal gene acquisition by B. pseudomallei is an important feature of recent genetic evolution and that this has resulted in a genetically diverse pathogenic species.
Assuntos
Burkholderia pseudomallei/genética , Melioidose/microbiologia , Adulto , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Composição de Bases , Sequência de Bases , Burkholderia pseudomallei/metabolismo , Burkholderia pseudomallei/patogenicidade , Cromossomos Bacterianos/fisiologia , Metabolismo Energético/genética , Evolução Molecular , Feminino , Genoma Bacteriano , Ilhas Genômicas/genética , Humanos , Dados de Sequência Molecular , VirulênciaRESUMO
Aspergillus fumigatus is the most ubiquitous opportunistic filamentous fungal pathogen of human. As an initial step toward sequencing the entire genome of A. fumigatus, which is estimated to be approximately 30 Mb in size, we have sequenced a 922 kb region, contained within 16 overlapping bacterial artificial chromosome (BAC) clones. Fifty-four percent of the DNA is predicted to be coding with 341 putative protein coding genes. Functional classification of the proteins showed the presence of a higher proportion of enzymes and membrane transporters when compared to those of Saccharomyces cerevisiae. In addition to the nitrate assimilation gene cluster, the quinate utilisation gene cluster is also present on this 922 kb genomic sequence. We observed large scale synteny between A. fumigatus and Aspergillus nidulans by comparing this sequence to the A. nidulans genetic map of linkage group VIII.
Assuntos
Aspergillus fumigatus/genética , Aspergillus fumigatus/metabolismo , Genoma Fúngico , Nitratos/metabolismo , Aspergillus nidulans/genética , Cromossomos Artificiais Bacterianos , DNA Fúngico/química , DNA Fúngico/isolamento & purificação , DNA Intergênico , Enzimas/genética , Enzimas/metabolismo , Proteínas Fúngicas/genética , Proteínas Fúngicas/fisiologia , Ordem dos Genes , Genômica , Íntrons , Proteínas de Membrana Transportadoras/genética , Proteínas de Membrana Transportadoras/fisiologia , Dados de Sequência Molecular , Família Multigênica , Fases de Leitura Aberta , Ácido Quínico/metabolismo , Saccharomyces cerevisiae/genética , Análise de Sequência de DNA , SinteniaRESUMO
The African trypanosome, Trypanosoma brucei, causes sleeping sickness in humans in sub-Saharan Africa. Here we report the sequence and analysis of the 1.1 Mb chromosome I, which encodes approximately 400 predicted genes organised into directional clusters, of which more than 100 are located in the largest cluster of 250 kb. A 160-kb region consists primarily of three gene families of unknown function, one of which contains a hotspot for retroelement insertion. We also identify five novel gene families. Indeed, almost 20% of predicted genes are members of families. In some cases, tandemly arrayed genes are 99-100% identical, suggesting an active process of amplification and gene conversion. One end of the chromosome consists of a putative bloodstream-form variant surface glycoprotein (VSG) gene expression site that appears truncated and degenerate. The other chromosome end carries VSG and expression site-associated genes and pseudogenes over 50 kb of subtelomeric sequence where, unusually, the telomere-proximal VSG gene is oriented away from the telomere. Our analysis includes the cataloguing of minor genetic variations between the chromosome I homologues and an estimate of crossing-over frequency during genetic exchange. Genetic polymorphisms are exceptionally rare in sequences located within and around the strand-switches between several gene clusters.