Widespread Pesticide Distribution in the European Atmosphere Questions their Degradability in Air.

Risk assessment of pesticide impacts on remote ecosystems makes use of model-estimated degradation in air. Recent studies suggest these degradation rates to be overestimated, questioning current pesticide regulation. Here, we investigated the concentrations of 76 pesticides in Europe at 29 rural, coastal, mountain, and polar sites during the agricultural application season. Overall, 58 pesticides were observed in the European atmosphere. Low spatial variation of 7 pesticides suggests continental-scale atmospheric dispersal. Based on concentrations in free tropospheric air and at Arctic sites, 22 pesticides were identified to be prone to long-range atmospheric transport, which included 15 substances approved for agricultural use in Europe and 7 banned ones. Comparison between concentrations at remote sites and those found at pesticide source areas suggests long atmospheric lifetimes of atrazine, cyprodinil, spiroxamine, tebuconazole, terbuthylazine, and thiacloprid. In general, our findings suggest that atmospheric transport and persistence of pesticides have been underestimated and that their risk assessment needs to be improved.

Digest, stain and bleach: Three steps to achieving rapid microplastic fluorescence analysis in wastewater samples.

Efforts associated with common analytical techniques for microplastics including spectroscopic and thermo-analytical techniques are limiting the ability to perform large-scale monitoring of microplastics in the aquatic environment, because the analytical equipment required is costly and the analysis itself time consuming. Thus, there is a need to develop low cost, rapid alternative monitoring approaches. One possible alternative is the use of selective fluorescence staining of microplastic particles directly applied to environmental samples. However, to the best of our knowledge this has not yet been successfully implemented for wastewater samples. In this study, sludge samples are used as surrogates for wastewater alongside six different polymers to develop a combined sample preparation and staining protocol that could selectively stain microplastics without significant interference from the natural constituents of the sludge. Results confirmed that using Fenton's reagent to remove the organic matter before staining the sample with Nile red (NR) and subsequently bleaching it by sodium hypochlorite resulted in the best workflow to selectively stain microplastics and then analyze them in wastewater samples using fluorescence microscopy.

Reply to Letter to the Editor: Huang, M., 2022: Comments on "Persistent, bioaccumulative, and toxic chemicals in insects: Current state of research and where to from here?". Sci Total Environ. 838, 155624, and Corrigendum.

In our recently published study in Science of the Total Environment, we used a systematic literature search to investigate the current state of research of persistent, bioaccumulative, and toxic chemicals (PBTs) and insects. We found a distinct increase of human, animal, and vertebrate publications related to PBTs in the early 1990s but did not identify a conclusive cause for this. In her Letter to the Editor, Huang (2022) offered an explanation for our result, and we have used her initiative to repeat our analysis with refined methodology. We present a corrected version of our original Fig. 1; importantly, though, our main finding, the general underrepresentation of insects in the research of PBTs, has remained the same. We conclude by addressing difficulties such as the reproducibility in literature searches and by discussing consequences of unequal resource distributions in science.

Microplastic sampling from wastewater treatment plant effluents: Best-practices and synergies between thermoanalytical and spectroscopic analysis.

Wastewater treatment plants (WWTPs) may represent point sources for microplastic discharge into the environment. Quantification of microplastic in effluents of WWTPs has been targeted by several studies although standardized methods are missing to enable a comparability of results. This study discusses theoretical and practical perspectives on best practices for microplastic sampling campaigns of WWTPs. One focus of the study was the potential for synergies between thermoanalytical and spectroscopic analysis to gain more representative sampling using the complementary information provided by the different analytical techniques. Samples were obtained before and after sand filtration from two WWTPs in Germany using cascade filtration with size classes of 5,000 - 100 µm, 100 - 50 µm, and 50 - 10 µm. For spectroscopic methods samples were treated by a Fenton process to remove natural organic matter, whereas TED-GC-MS required only sample extraction from the filter cascade. µFTIR spectroscopy was used for the 100 µm and 50 µm basket filters and µRaman spectroscopy was applied to analyze particles on the smallest basket filter (10 µm). TED-GC-MS was used for all size classes as it is size independent. All techniques showed a similar trend, where PE was consistently the most prominent polymer in WWTP effluents. Based on this insight, PE was chosen as surrogate polymer to investigate whether it can describe the total polymer removal efficiency of tertiary sand filters. The results revealed no significant difference (ANOVA) between retention efficiencies of tertiary sand filtration obtained using only PE and by analyzing all possible polymers with µFTIR and µRaman spectroscopy. Findings from this study provide valuable insights on advantages and limitations of cascade filtration, the benefit of complementary analyses, a suitable design for future experimental approaches, and recommendations for future investigations.

Persistent, bioaccumulative, and toxic chemicals in insects: Current state of research and where to from here?

The ongoing decline in the biomass, abundance, and species number of insects is an established fact. Persistent, bioaccumulative, and toxic chemicals (PBTs) - persistent organic pollutants (POPs) and, in the case of our study, mercury (Hg) - play an important role, but their effect on insect populations is insufficiently investigated. Here, the current state of research on PBTs related to insects is examined with a systematic literature study using Web of Science™. We investigate time trends of research intensity compared with other organisms, insect orders and chemicals analyzed, chemicals' effects on insects, and geographical aspects. We show that research intensity increased in the early 1990s, but studies on PBTs in insects are still underrepresented compared with other organisms. The taxonomic focus lies strongly on dipterans. The predominance of studies on DDT suggests its relevance in the context of disease-vector management. Phenotypic and acute effects on insects were more often investigated than genotypic and chronic effects. Laboratory-bred insects and wild-bred insects were examined equally often, pollutant exposure and analysis were conducted predominantly in the laboratory. Mostly habitats with a medium or high human impact were studied, and natural and near-natural habitats are understudied. The sources of the substances are often unknown. Most studies were carried out in economically rich continents, including North America, Europe, and Australia. The numbers of publications dealing with Asia, South America, and Africa are comparatively low, although the control of vector-borne diseases with POPs is still intensively practiced there. We identify gaps in the research - among others, refined analytical methods for biomarkers and for the examination of chronic effects, combinations of field and laboratory experiments to analyze the same problem, and a global approach for the monitoring of PBTs will be needed for accelerating the dearly needed progress in the research of PBTs in insects.

Persistent, Bioaccumulative, and Toxic Chemicals in Wild Alpine Insects: A Methodological Case Study.

With their high persistence in the environment and their potential for long-range atmospheric transport, persistent, bioaccumulative, and toxic chemicals (PBTs) may be among the numerous anthropogenic threats to insect populations worldwide. The effects of PBTs on insects have been investigated in the laboratory, but topical field studies are scarce. A reason might be the multiple challenges faced by PBT-related field studies on wild insects. We studied two species of bumblebees (Bombus spp.) and of ants (Formica spp.) in two high-elevation locations in the Austrian and German Alps to tackle two of these challenges. First, PBTs occur in minuscule concentrations compared with other substances in the environment. Therefore, the practicability of body burden data from pooled individuals was tested. Second, fitness proxies like fecundity, which typically are endpoints for chemical toxicity, are difficult to quantify in the field. Hence, fluctuating asymmetry of bumblebee wings and ant heads was tested as an alternative endpoint. To exclude the possibility that fluctuating asymmetry was caused by genetic stressors, inbreeding levels were estimated using population-genetic markers, and their relationships to fluctuating asymmetry in the same individuals were assessed. We successfully quantified polychlorinated biphenyls and Hg as PBTs using the pooled samples and found PBT data from pooled individuals useful, in that significant correlations to fluctuating asymmetry were identified in bumblebees and ants. This finding confirmed the potential of fluctuating asymmetry to indicate PBT effects in wild insects. Inbreeding did not interfere with PBT links to fluctuating asymmetry in any instance. Our findings contribute to the development of a quantitative methodological framework for investigating the effects of persistent environmental chemicals on wild insects. Environ Toxicol Chem 2022;41:1215-1227. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

When Good Intentions Go Bad-False Positive Microplastic Detection Caused by Disposable Gloves.

Apart from being considered a potential threat to ecosystems and human health, the ubiquity of microplastics presents analytical challenges. There is a high risk of sample contamination during sampling, sample preparation, and analysis. In this study, the potential of sample contamination or misinterpretation due to substances associated with disposable laboratory gloves or reagents used during sample preparation was investigated. Leachates of 10 different types of disposable gloves were analyzed using Raman microspectroscopy (µ-Raman), Fourier-transform infrared microspectroscopy (µ-FTIR), and pyrolysis-gas chromatography/mass spectrometry (pyr-GC/MS). There appeared to be polyethylene (PE) in almost all investigated glove leachates and with all applied methods. Closer investigations revealed that the leachates contained long-chain compounds such as stearates or fatty acids, which were falsely identified as PE by the applied analytical methods. Sodium dodecyl sulfate, which is commonly applied in microplastic research during sample preparation, may also be mistaken for PE. Therefore, µ-Raman, µ-FTIR, and pyr-GC/MS were further tested for their capability to distinguish among PE, sodium dodecyl sulfate, and stearates. It became clear that stearates and sodium dodecyl sulfates can cause substantial overestimation of PE.

Getting on with persistent pollutants: Decreasing trends of perfluoroalkyl acids (PFAAs) in sewage sludge.

Sewage sludge can be a relevant source of perfluoroalkyl acids (PFAAs) for the environment. In order to reduce emissions from this source, Bavarian authorities enforced in 2008 an analysis of PFAAs from sewage sludge derived from municipal wastewater treatment plants (WWTPs). 4981 sludge samples from 1165 different WWTPs were analyzed between 2008 and 2013 for 11 PFAAs compounds. During this period, 71 WWTPs exceeded the precautionary limit of 125 µg kg(-1) dm of total PFAAs in sludge samples at least once with a decreasing tendency. The yearly exceedances of the investigated WWTPs decreased from 6% in 2008 to 0.8% in 2013. At the same time, the percentage of uncontaminated WWTPs increased from 33% to 65%. Perfluorooctane sulfonic acid (PFOS) was the predominant compound found in 41% of all sludge samples. Perfluorodecanoic acid (PFDA) was detected in 19% and Perfluorooctanoic acid (PFOA) in 7%. Very high PFAAs concentrations (>500 µg kg(-1) dm) in sewage sludge were generally caused by firefighting foams containing PFAAs or emissions from PFAAs-using industries including metal plating, textile, leather or paper industries. Trend analyses of the six year period show that PFAAs contamination in sewage sludge clearly decreased for 47% of the WWTPs. However, for 16% of the WWTPs an increasing trend was detected, even though the concentration levels were below the precautionary limit. During the six years of investigation the load of total PFAAs in sewage sludge was reduced by more than 90%, from 17 t a(-1) in 2008 to 1.5 t a(-1) in 2013.