A comprehensive review of TGA transcription factors in plant growth, stress responses, and beyond.

TGA transcription factors (TFs), belonging to the D clade of the basic region leucine zipper (bZIP) family, exhibit a specific ability to recognize and bind to regulatory elements with TGACG as the core recognition sequence, enabling the regulation of target gene expression and participation in various biological regulatory processes. In plant growth and development, TGA TFs influence organ traits and phenotypes, including initial root length and flowering time. They also play a vital role in responding to abiotic stresses like salt, drought, and cadmium exposure. Additionally, TGA TFs are involved in defending against potential biological stresses, such as fungal bacterial diseases and nematodes. Notably, TGA TFs are sensitive to the oxidative-reductive state within plants and participate in pathways that aid in the elimination of reactive oxygen species (ROS) generated during stressful conditions. TGA TFs also participate in multiple phytohormonal signaling pathways (ABA, SA, etc.). This review thoroughly examines the roles of TGA TFs in plant growth, development, and stress response. It also provides detailed insights into the mechanisms underlying their involvement in physiological and pathological processes, and their participation in plant hormone signaling. This multifaceted exploration distinguishes this review from others, offering a comprehensive understanding of TGA TFs.

Highly dispersive Pt-Pd nanoparticles on graphene oxide sheathed carbon fiber microelectrodes for electrochemical detection of H2O2 released from living cells.

We report a facile strategy for the synthesis of surfactant-free, small and highly dispersive Pt-Pd nanoparticles on graphene oxide (Pt-Pd NPs/GO) by an electroless deposition method, which is sheathed on carbon fiber microelectrodes (CFMs) as an electrochemical sensing platform for highly sensitive and selective detection of hydrogen peroxide (H2O2) released from the living cells. GO serves as the reducing agent and stabilizer for electroless deposition of Pd NPs on the surface of GO owing to its low work function (4.38 eV) and highly conjugated electronic structure. The obtained Pd NPs/GO have a relatively high work function (4.64 eV), and thereby could be used as stabilizer for synthesis of surfactant-free, small and highly dispersive Pt-Pd NPs/GO by chemical reduction of K2PtCl4. The obtained Pt-Pd NPs have a uniform size of 4.0 ± 0.6 nm on the surface of GO. Moreover, the Pt-Pd NPs/GO sheathed CFMs exhibit an excellent electrocatalytic activity for the reduction of H2O2 with a low detection limit of 0.3 µM and good selectivity. These good properties enable the modified microelectrode to detect the H2O2 released from living cells.