RESUMO
The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.
Assuntos
Regulação Bacteriana da Expressão Gênica/fisiologia , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Técnica de Amplificação ao Acaso de DNA Polimórfico , Staphylococcus aureus/classificação , Staphylococcus aureus/genética , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Técnicas de Tipagem Bacteriana , Bovinos , Feminino , Mastite Bovina/microbiologiaRESUMO
Based on already published data, the following issues have been covered in the present review: epidemiology, epizootiology and the occurrence of anthrax in man with reference to current data. Moreover, in the paper are presented some problems of intestinal anthrax with emphasis on environment conditions, pathogenesis, prevention and treatment of this clinical form.
Assuntos
Antraz/complicações , Meio Ambiente , Doenças Transmitidas por Alimentos/epidemiologia , Doenças Transmitidas por Alimentos/microbiologia , Antraz/tratamento farmacológico , Antraz/epidemiologia , Feminino , Gastroenteropatias/microbiologia , Humanos , Masculino , Penicilinas/uso terapêuticoAssuntos
Cobaias/microbiologia , Yersiniose/epidemiologia , Yersinia/isolamento & purificação , Doença Aguda , Adolescente , Adulto , Animais , Antibacterianos/farmacologia , Argentina , Pré-Escolar , Fezes/microbiologia , Feminino , Humanos , Masculino , Yersinia/efeitos dos fármacos , Yersiniose/etiologiaAssuntos
Surtos de Doenças , Yersiniose/genética , Infecções por Yersinia pseudotuberculosis/genética , Adolescente , Adulto , Animais , Argentina , Pré-Escolar , Feminino , Cobaias , Humanos , Masculino , Doenças dos Roedores/transmissão , Infecções por Yersinia pseudotuberculosis/transmissão , ZoonosesRESUMO
On a commercial poultry farm, a large percentage (9%) of clinically healthy fowls had positive reaction to the plate test, with commercial polyvalent pullorum antigens. We could not isolate Salmonella from the positive birds. An strain, of Escherichia coli Balcarce (E. coli B) was isolated from the feces of one of the birds. The isolate was identified biochemically and the antigenic study showed correlation with E. coli 044 and the somatic fraction 1, 2, 8, 14 and 23 of the Salmonella genus. The common antigens were studied by agglutination, absorption and crossed immunodiffusion tests, comparing the isolated strain and the different Salmonella serotypes. Four pullorum polyvalent commercial antigens reacted with sera containing somatic agglutinins 1, and with the E. coli B antiserum. These observations confirm the high antigenic correlation between the genus of the Enterobacteriaceae family. It is indicated that for the diagnosis of avian salmonelosis rather than using a single serological tests, the isolation and identification of the etiological agent is required.