Evaluation of Prognostic Factors and Determinants in Surgical Sperm Retrieval Procedures in Azoospermic Patients.

INTRODUCTION: Main categorisation of azospermic is in two grups: nonobstructive azoospermia (NOA) and obstructive azoospermia (OA). We had evaluation of prognostic factors and determinants in sperm retrieval procedures in azoospermic patients. METHODS: Retrospective evaluation observed 21 selected patients with NOA and OA azoospermia, after that complete history, physical examination with ultarsound volume of testis and hormonal profile. Hormonal profile included: follicle stimulating hormone (FSH), luteinizing hormone (LH), testosteron (T) and prolactin (P) serum levels. Also karyotype and Y-deletion analysis were done and analyzed. RESULTS: 9 OA patients (42,9%) were undergone for TESE operation and 12 NOA patients (57,1%) for Micro-TESE operation. All TESE procedures were positive (100%). Micro-TESE in 12 selected NOA patients, 5 patients (41,6%) were positive and 7 patients (58,4%) negative. Patients testicular size, serum FSH and testosterone level showed correlation in success of sperm retrieval procedures. CONCLUSION: TESE is elected procedure for obstructive azospermia (OA). Micro-TESE is appropriate sperm retrieval procedurec for patients with non-obstructive azoospermia (NOA) and correlate with high FSH and small volume of testis.

The First Report from Bosnia and Herzegovina on Micro-tese Results in Azoospermic Patients.

INTRODUCTION: To present results of MicroTese procedure in treatment of male infertility in patients with azoospermia at Bahçeci BIH IVF Center during two-year period of 2013-2014. METHODS: In the stated time-period, 55 MicroTese surgeries were performed. In 52.7% of the cases, sperm cells were isolated after MicroTese surgery, and in 47.3% of the cases, there was a negative outcome of the procedure. Obtained sperm cells were subjected to cryopreservation. Furthermore, ICSI procedure was performed by use of the obtained sperm cells. RESULTS: Of 29 positive MicroTese surgeries, 21 (72.4%) resulted in clinical pregnancies. Biggest percentage of negative MicroTese procedures happened in patients with cryptorchidism and orchidopexy. CONCLUSION: MicroTese is the most precise and successful method of retrieving sperm cells surgically in men with azoospermia. Our results are within scope of results in referent world centers.

Comparison of gender-specific human embryo development characteristics by time-lapse technology.

Numerous studies indicate that there might be differences in embryo growth dynamics between male and female embryos. However, current data in humans are scarce and the results are inconclusive or conflicting. This study asks whether there exist gender-specific embryo development kinetics or parameters between human male and female embryos that can be observed by time-lapse technology. Study included data from 139 consecutive cycles (177 embryos transferred, 179 sacs analysed) with positive pregnancy that resulted in 100% implantation. Single- or double-embryo transfers were performed. Cases were analysed for parameters including cleavage time points and duration in each cleavage from two cells to hatching blastocyst stages and time interval between cleavages. Morphokinetic parameters of 78 female and 60 male embryos from a total of 119 cycles (139 sacs were examined after transfer of 138 embryos) were processed for data analysis according to the gender group. A detailed analysis of the data regarding each time point or interval between consecutive events according to these groups showed them to be similar in cell division kinetics, from the early cleavage through their development to blastocyst stage. However, female embryos showed earlier cavitation than male embryos, but the results did not reach statistical significance.

Time-lapse evaluation of human embryo development in single versus sequential culture media--a sibling oocyte study.

OBJECTIVE: To compare the dynamics of early development between embryos cultured in single and sequential media. DESIGN: Randomized, comparative study. SETTING: Private IVF centre. PATIENTS: A total of 446 metaphase II oocytes from 51 couples who underwent oocyte retrieval procedure for intracytoplasmic sperm injection. Forty-nine resulted in embryo transfer. INTERVENTION: Oocytes were split between single and sequential media produced by the same manufacturer and cultured in a time-lapse incubator. MAIN OUTCOME MEASURES: Morphokinetic parameters until the embryos reached the 5-cell stage (t5), utilization, clinical pregnancy and implantation rates. RESULTS: Embryos cultured in single media were advanced from the first mitosis cycle and reached 2- to 5-cell stages earlier. There was not any difference between the durations for cell cycle two (cc2 = t3-t2) and s2 (t4-t3). The utilization, clinical pregnancy and implantation rates did not differ between groups. The proportion of cryopreserved day 6 embryos to two pronuclei oocytes was significantly higher in sequential than in single media. CONCLUSIONS: Morphokinetics of embryo development vary between single and sequential culture media at least until the 5-cell stage. The overall clinical and embryological parameters remain similar regardless of the culture system.