Neuroprotective effects of the combined treatment of resveratrol and urapidil in experimental cerebral ischemia-reperfusion injury in rats.

PURPOSE: To evaluate the neuroprotective effect of resveratrol, urapidil, and a combined administration of these drugs against middle cerebral artery occlusion (MCAO) induced ischemia/reperfusion (IR) injury model in rats. METHODS: Thirty-five rats were divided into five groups of seven animals each. Animals in IR, IR resveratrol (IRr), IR urapidil (IRu), and IR + combination of resveratrol and urapidil (IRc) were exposed to MCAO induced cerebral ischemia reperfusion injury model. Rats in IRr and IRu groups received 30-mg/kg resveratrol and 5-mg/kg urapidil respectively. Animals in IRc received a combined treatment of both drugs. At the end of the study, brain tissues were used for oxidative stress (malondialdehyde, glutathione, and superoxide dismutase), pro-apoptotic caspase-3, anti-apoptotic Bcl-2, and pro-inflammatory tumor necrosis factor-α cytokine level measurements. RESULTS: The MCAO model successfully replicated IR injury with significant histopathological changes, elevated tissue oxidative stress, and upregulated apoptotic and inflammatory protein expression in IR group compared to control group (p < 0.001). All parameters were significantly alleviated in IRr group compared to IR group (all p < 0.05). In IRu group, all parameters except for caspase-3 and Bcl-2 were also significantly different than IR group (all p < 0.05). The IRc group showed the biggest difference compared to IR group in all parameters (all p < 0.001). The IRc had higher superoxide dismutase and Bcl-2 levels, and lower caspase-3 levels compared to both IRr and IRu groups (all p < 0.05). Also, the IRc group had lower MDA and TNF-α levels compared to IRu group (all p < 0.05). CONCLUSIONS: The results indicate that combined treatment of resveratrol and urapidil may be a novel strategy to downregulate neurodegeneration in cerebral IR injury.

Developmental three-dimensional examination of the pelvic cavity of Hamdani crossbred sheep fetuses (Ovis aries) in the last two periods of gestation.

BACKGROUND: Türkiye is a country in the world ranking in terms of sheep breeding. Hamdani crossbred sheep breed is one of the sheep breeds that can adapt to the difficult conditions in our country. In addition, the sentence may be corrected as 'Especially in the southeastern part of the Türkiye, crossbreeding is preferred by breeders to increase the yield characteristics of sheep'. OBJECTIVES: In our study, it was aimed to perform a pelvimetric analysis of Hamdani crossbred sheep fetuses in the second and third trimesters by three-dimensional modelling method. METHODS: For this purpose, a total of 40 second-trimester (10 females, 10 males) and third-trimester (10 females, 10 males) fetuses were used. The pelvises of the fetuses were imaged with CT and 3D models were created. Sixteen pelvimetric measurements were performed from the models. The results obtained were evaluated statistically. RESULTS: The study found statistically significant differences between male and female fetuses in terms of vertical diameter (VD) and foramen obturatum width (FOW) measurement parameters in the second trimester. In the third trimester, there were statistically significant differences between genders in acetabulum (AC) (p < 0.01), medial ischial tuberosities (MIT), and ischiatic arch (IA) (p < 0.05) parameters. As a result of the correlation analysis, it was determined that the pelvis length (PL) measurement parameter was positively correlated with different parameters in varying degrees in both periods, while the IA parameter did not show significant correlations with the other parameter in the last two periods of pregnancy. CONCLUSION: In conclusion, the data obtained will be useful in pelvimetric evaluations of human and animal fetuses, anatomy education, zooarchaeology and taxonomy studies.

Comparison of Skull Morphometric Characteristics of Simmental and Holstein Cattle Breeds.

This study aimed to reveal the morphological characteristics of pure Holstein and Simmental skulls and to obtain reference values for morphometric analysis. Moreover, 54 skulls from 12- to 14-month-old male Holstein (n = 25) and Simmental (n = 29) cattle were collected from Turkey's Southeastern Anatolia Region between 2023 and 2024. Linear measurements indicated that Holsteins had longer skulls compared to Simmentals. Holsteins exhibited significantly higher values for the greatest length of nasals and the shortest skull length. The facial breadth was wider in Holsteins and statistically distinctive between the breeds. Holsteins had a more oval orbital bony roof, while Simmentals exhibited a wider orbital structure. The orbital index was higher in Holsteins, distinguishing between the two breeds. It was observed that Simmental cattle had a wider occipital region. This difference is likely due to the larger lateral appearance of the Simmental skull, which has more body weight and provides a larger surface area for muscle attachment. These differences not only aid in breed identification but also offer insights into the functional adaptations of each breed. Future research should explore the genetic and environmental factors contributing to these morphological traits, further enriching our knowledge of cattle morphology and its implications for breeding and conservation efforts.

Size and shape of the neurocranium of laying chicken breeds.

The neurocranium in birds provides valuable insights into their morphological diversity, including adaptations related to brain size, facial shaping, and environmental factors. This study analyzes the neurocranial shape characteristics and size of chickens with similar genetic backgrounds. By examining the neurocranial shape variation in chickens of the same age and sex, the study aims to understand the factors contributing to morphological diversity within this specific group. 3D geometric morphometrics was used to analyze 235 neurocrania from four chicken breeds. The analysis revealed significant differences in centroid size among the chicken breeds. The largest neurocranium centroid size was found in Sasso chickens., which were statistically separated from Atak-S. Additionally, centroid size effectively differentiates between Lohmann Brown and Lohmann Sandy chicken breeds. The most significant shape variation concerned the width of the rostral part of the frontal bone. However, according to the PC1 value, the shape variation was observed within rather than between groups. Lohmann Sandy chickens exhibited higher variability in neurocranial shape, suggesting greater shape diversity within this breed than others. As for shape analysis, the breeds showed closer similarity to each other. Lohmann Sandy chickens are characterized by positive PC1 value, with the rostral end of the frontal region inclined more ventrally, and a more extensive basioccipital region. Sasso chickens have a more dome-shaped middle part of the frontal region than other breeds. The study also identified the most significant shape variation among the study samples, observed at the rostral part of the frontal bone. These findings contribute to understanding the genetic and environmental influences shaping neurocranial morphology in chickens. Similar studies in different bird species and subspecies offer valuable insights into avian biology and adaptation.

Morphometric, macroscopic and microscopic investigation of glandulae uropygiale in turkeys (Meleagris gallopavo).

This study was carried out to investigate the macroanatomical, morphometric, histological, and electron microscopic characteristics of the uropygial gland in adult male and female turkeys (Meleagris gallapovo).

Evaluation and 3D imaging of mineral structure changes of the rabbit (New Zealand) skull during developmental periods.

This study aimed to determine the morphometric measurements anatomically and CT images of skulls of healthy male and female rabbits during postnatal development, to analyse the data statistically and to demonstrate the structural changes in bone. A total of 40 rabbits (20 females and 20 males) were divided into four groups including prepubertal period (group I (0-1 month)), period between adolescence and adulthood (group II (3-5 month)) and later (young adult period as group III (1-3 years) and old adult period as group IV (3-5 years)), with five animals in each group. After the morphometric measurements, the surface area and volume values of the skull were calculated. The skulls were reconstructed using a 3D Slicer (5.0.2), which is used for 3D modelling. The cranial bones in each group were then crushed using a grinder so that the powdered samples were obtained for XRF (X-ray fluorescence technique). The p-value was statistically highly significant between group and gender (p < 0.001). In morphometric measurements, males were generally higher than females. Only PL, GBOC and GNB measurements were higher in females. The p-value between groups (in all measurements), between genders (in TL, GLN, FL, VL, OZB and GBN parameters) and between groups and genders (in TL, DL and VL parameters) was statistically highly significant (p < 0.001). The p-value between the groups, p-value between sexes and p-value between group and sex in Si, P, K, Ca, Ni, Zn, Sr, Sr and Ca/P elements were statistically significant (p < 0.001). Consequently, metric, volume and surface area measurements were taken through 3D modelling of skull bone in prepubertal period (group I), period between adolescence and adulthood (group II) and later (young adult period as group III and old adult period as group IV) of rabbits and the change in the mineral structure during postnatal development and effect of sex on this change were investigated. This might be the first study to assess both metric and mineral changes at four age intervals taken during the life span of rabbits.

Examination of upper respiratory tracts of Siirt-coloured mohair goats by scanning electron microscopy before and after plastination.

Present study was conducted to determine the changes in the surface structure of the upper respiratory tract of Siirt-coloured mohair goats by the silicone plastination method. Accordingly, the heads of 10 Siirt-coloured mohair goats procured from slaughterhouses were divided into two halves. Half of each head was plastinated. After macro-comparisons were made, the deformations of silicone plastination on the surface were examined by comparing the scanning electron microscope (SEM) findings of both upper respiratory tract tissue samples collected from plastinates and fresh material. When the data from scanning electron microscopy were analysed, cilia, cobblestone patterns, goblet cells and gland ducts on the epithelial surface were identified in areas on the upper respiratory tract. The SEM images of the plastinated tissues showed that the surface structures were degenerated due to the deformation of the surface epithelium. The plastination technique damaged the structures on the surface epithelium. Since the plastination technique and scanning electron microscopy have been studied together for the first time, we believe this would contribute to the scientific literature.

Ecotoxicological risk assessments and components of persistent organic pollutants and metals in the historical settlement area (Iznik (Nicea) lake) large water resource sediments.

The main objectives of this study are to measure permanent organic and inorganic pollutants in detail in an area that hosts historical structures underneath and feeds the huge ecosystem with water, to reveal risk values. Total PAH concentrations in the samples ranged from 43.41 to 202.7 ng/g. Total OCP concentration ranged from 5.15 to 17.98 ng/g, while total PCB concentration ranged from 0.179 to 0.921 ng/g. PCB 28/31, 138, and 153 are the highest detected PCBs. It was found that the lake sediment reached toxic equivalent quotient (TEQ) values of 29.21 for total PAHs and 28.90 for carcinogenic PAHs. Negligible concentration risk quotient had a low to moderate ecological and toxicological risk between 12.91 and 64.42. Highest pollution index value was found 3.81 and the risk index value reached 417.4. It has been revealed that toxicologically risky components accumulate over many years even in the best-protected water resources.

Evaluation of hexabromocyclododecane (HBCD), polybrominated dibenzo-p-dioxins/furans (PBDD/Fs) and polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) outflows during the destruction of HBCD wastes in a hazardous waste incinerator.

This paper presents an evaluation of hexabromocyclododecane (HBCD), polybrominated dibenzo-p-dioxins/furans (PBDD/Fs) and polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) outflows during the destruction of HBCD waste stockpiles in IZAYDAS Hazardous Waste Incinerator (HWI) in Kocaeli, Türkiye. HBCD wastes containing 100 % pure HBCD were in 25 kg packages with 63 % Br content were co-incinerated in a 3-day test burn with average feed rate of 26 kg/h. HBCD, PBDD/Fs and PCDD/Fs were measured in the outlet streams to quantify the amount of unintended POPs releases associated with the processing of HBCD waste and to observe the POP removal performance of air pollution control equipment (APCE) of the incinerator. Total mass outflow rate of HBCDs is calculated as 2.6 g/day, corresponding to destruction efficiency of 99.9996 %. Total toxicity of the brominated dioxins was measured as 0.00044 ng TEQ/Nm3 on average, while highly brominated congeners are dominant. PCDD/F concentrations in the outflow streams during HBCD test burns are produced similar congener distributions with those given in the previous studies, with the dominance of 7,8-chlorinated congeners. Mass flows in the outlet streams indicated that the efficiency of ESP and wet scrubbers for the removal of PCDD/Fs and HBCDs. Flue gas concentrations of PCDD/Fs, HBCDs and PBDD/Fs obtained in HBCD burn test indicated that burning HBCD wastes cause no significant emissions as operational parameters and total halogen content in the menu are kept within the incinerator limits.

Analysis and 3D Imaging of Morphometric - Mineral Structure Changes in Skulls of Male and Female Guinea Pigs During and After Developmental Period

SUMMARY: Even though morphometric and mineral studies related to the guinea pig (Cavia porcellus) skull have been carried out, this study is the first attempt to evaluate all developmental stages of male and female guinea pigs. This study aims to this study is to create 3D modeling of CT images obtained from the skulls of male and female guinea pigs during the developmental period (prepuberty and the period between puberty and adulthood) and following periods (young adulthood and old adulthood), to analyze some biometric bone data such as volume, surface area and length, and to assess the developmental analysis of the mineral matter change in their skulls. The CT-scanned skulls were transferred to 3D Slicer (5.0.2), which is used for 3D modeling. The surface area and volume were calculated by measuring the measurement points on the models. In addition, the XRF device was used to show elemental ratio changes during different developmental stages. According to metric measurements, a gradual increase was observed during the life period. The metric measurements of the skull bone had a higher measurement value in male guinea pigs than in their female counterparts. While Ca/P ratio increased up to the third group and partially decreased in the fourth group in males, it gradually increased from the first group to the fourth group in females. This study revealed that puberty, adulthood and sex were effective in the physical and chemical characterization of skull bone structure in guinea pigs.

Aunque se han realizado estudios morfométricos y de minerales relacionados con el cráneo del cobayo (Cavia porcellus), esta investigación es el primer intento de evaluar las etapas de desarrollo de cobayos machos y hembras. El objetivo de este estudio fue crear un modelado 3D de imágenes de tomografía computarizada obtenidas de los cráneos de cobayos machos y hembras durante el período de desarrollo (prepubertad y el período entre la pubertad y la edad adulta) y los períodos siguientes (edad adulta joven y edad adulta mayor), para analizar algunos datos biométricos de los huesos, como el volumen, la superficie y la longitud, y además, analizar el cambio de materia mineral en sus cráneos durante el desarrollo. Los cráneos escaneados se transfirieron a 3D Slicer (5.0.2), que se utiliza para el modelado 3D. El área de superficie y el volumen se calcularon midiendo los puntos de medición en los modelos. Además, se utilizó el dispositivo XRF para mostrar los cambios en las proporciones elementales durante diferentes etapas de desarrollo. Según mediciones métricas, se observó un aumento gradual durante el período de vida. Las medidas métricas del hueso del cráneo tuvieron un valor de medición más alto en los cobayos machos que en las hembras. Mientras que la relación Ca/P aumentó hasta el tercer grupo y disminuyó parcialmente en el cuarto grupo en los machos y aumentó gradualmente del primer grupo al cuarto grupo en las hembras. Este estudio reveló que la pubertad, la edad adulta y el sexo fueron efectivos en la caracterización física y química de la estructura ósea del cráneo en cobayos.

Morphometric analysis of the skulls of a ram and ewe Romanov sheep (Ovis aries) with 3D modelling.

BACKGROUND: With the development of technology, 2D images have left their place for 3D models. The 3D modelling technique is widely used in plastic surgery, orthopaedic surgery, neurosurgery, traumatology, dentistry and medical education. The skull is important in terms of containing the starting parts of systems with vital functions. OBJECTIVE: The aim of the study is to reveal the difference between male and female and other species by 3D (three-dimensional) modelling and craniometric measurements of Romanov heads. METHODS: In our study, skulls of Romanov sheep (10 females and 10 males) older than 1-year-old were used. The heads of Romanov sheep were scanned with computed tomography and modelled in 3D. RESULTS AND CONCLUSIONS: In the study, it was determined that there was a statistically significant difference between male and female sheep in terms of the largest nose length, facial width, molar row length, viscerocranium length, and foramen magnum height parameters (p < 0.05). It was determined that the greatest width of the foramen magnum measurement parameter and the skull index showed statistically significant differences between the genders at the p < 0.01 level. No statistically significant difference was found in other measurements (p > 0.05). The data obtained as a result of the study will help in the racial discrimination and classification of bones obtained from zoo archaeological excavations.

Three-dimensional modelling and morphometric analysis of skull of badger (Meles meles) with computed tomography images.

BACKGROUND: Morphometric measurements help to understand the skull morphology in different animal species, detect skull deformations and figure out their causes. OBJECTIVES: The aim of this study is to make a three-dimensional (3D) modelling of the badger skull using computed tomography, to show its anatomical structures and to reveal the morphometric measurement values. METHODS: Skull measurements were carried out using a digital calliper at 27 measurement points based on metric measurement points. After the scanned images were stored in Digital Imaging and Communications in Medicine format, they were transferred to MIMICS 20.1 (The Materialize Group) programme and 3D models the skulls were created. The surface area and volume values of the skulls were calculated on these models. RESULTS: The volume and surface area parameters were expressed as mean ± SE. Four different indices were calculated using osteometric measurements. Statistical analyses were made by determining the mean value and standard deviation of the examined properties and the correlation coefficients among these properties. In the metric measurement points taken as a basis, the longest measurement in the skulls was the total length and its mean value was 124.60 ± 0.64 mm. The shortest measurement was the breadth dorsal to the external auditory meatus, and its mean value was 6.75 ± 0.24 mm. CONCLUSIONS: Consequently, statistical differences in the craniometric values of badgers' skulls were determined by using CT and 3D modelling software. In addition, it was concluded that the badger skull can be easily distinguished from other carnivore species by carrying out morphometric measurements.

Osteometric and topographic measurement of the skull and mandible of Siirt colored Mohair goat with three-dimensional (3D) modeling technique.

Siirt-colored Mohair goat is one of the breeds that contributed significantly to the existence of Mohair goats reared in Turkey. Morphological and morphometric characteristics of the Siirt-colored Mohair goat remained vague owing to a lack of studies. Recent advances in high-tech imaging have replaced conventional two-dimensional anatomical structures with three-dimensional (3D) models. In our study, morphometric features were determined by 3D modeling from computed tomography images obtained from the skull and mandibular bones of Siirt-colored Mohair goats. For this purpose, the skulls and mandibular bones of 20 Siirt-colored Mohair goats (10 females and 10 males) were used. The images were reconstructed with the help of a particular software program. The craniometric data were analyzed in terms of sexual dimorphism, and statistically significant difference was found in the A5, A18, and A31 measurement parameters (P<0.05) and Skull index (P<0.01) parameters. In the mandible measurements, there was a statistically significant difference between the sexes in C5, C10 measurement points (P<0.05), C2, C8, C12, C18, C21 measurement points (P<0.001) and surface area parameter (P<0.01). The morphometric data obtained is a resource in the fields of zoo archaeology, anatomy, forensics, anesthesia, surgery, and treatment.

Radiological examination of the skull of Siirt-coloured mohair goat, Romanov and Hamdani sheep.

The skull is a vitally important complex structure formed by the combination of many bones. Craniometric studies are performed with various imaging methods to determine morphometric characteristics in animals. One of these methods is radiography. In our study, skulls of 14 Hamdani sheep, 20 Siirt-coloured mohair goats and 20 Romanov sheep breeds were used. Skull sections were taken with a computed tomography device and saved in DICOM format. Nine separate measurements and six index calculations were made from the images. In the study, firstly, the gender difference was ignored and the similarities and differences between the species were recorded. In addition, gender differences were determined statistically, without making any distinction between species. Correlation analysis was performed to compare measurement parameters between animal species. As a result of the study, the highest skull length (SL) and cranial length (CL) values were determined in the Siirt-coloured mohair goat. It was determined that the difference between Romanov sheep and Siirt-coloured mohair goat in CL parameter was not statistically significant (p > 0.05). In addition, a statistically significant difference was observed between the Romanov sheep breed and the Hamdani sheep breed (p < 0.01). The external length of the cranium (ELC) measurement parameter was found to have a statistically significant difference in three animals (p < 0.01). According to the results of the correlation analysis, the highest correlation value was determined between cranial width (CW) and CL parameters (r = 0.920). When the species difference was ignored, and the statistical difference of the internal height of the cranium (IHC) parameter was found to be significant between female and male animals (p < 0.05). In addition, it was determined that SL, CW and internal cranium index (ICI) parameters had a significant difference depending on sex (p < 0.01). It is foreseen that the obtained data can be used in sciences such as zoo archaeology, anatomy, surgery and forensic medicine.

Physical and chemical characterization of the femur during and after the body development period in male and female guinea pigs.

In this study, it was aimed to reveal the physical and chemical characterization of the bone structures during body development periods (prepubertal period, period between adolescence and adulthood) and after (young adult period and old adult period) in male and female guinea pigs. In this study, 40 guinea pigs (20 male, 20 female) were used. Morphometric measurements, X-ray fluorescence (XRF) analysis for mineral levels, Brunauer-Emmett-Teller (BET) analysis for surface area, and porosity analysis were applied to the bones. The male guinea pigs had greater values than females in the other three categories, with the exception of the second group, when the females have higher values in morphometric measurements. Ca levels rose up to the third group, as did P levels in the males, peaking in the third group and declining in the fourth. As with phosphorus, there was a progressive rise in females from the first to the fourth group. Fe, Zn, and Sr elements had the greatest values in both genders in the first group. In all four groups, the females had greater Zn levels than males. The highest Ca/P ratio was found in the third male group and the fourth female group. This study revealed that adolescence, adulthood, and gender are effective in the physical and chemical characterization of bone structure in guinea pigs.

Morphometric analysis of the mandible of ram and ewe romanov sheep (Ovis aries) with 3D modelling: A CT study.

2D images view hardly measurement points due to the overlap of anatomical features. This challenge is overcome by 3D modelling. In particular, images obtained by computed tomography are converted into 3D models through certain software. In sheep breeds with high polymorphism, some changes have occurred in their morphology due to both environmental and genetic factors. In this context, determining the osteometric measurements of sheep and revealing breed-specific characteristics provide very important data for forensic, zooarchaeological, and developmental sciences. Mandibular reconstruction measurements are used to reveal differences between species and between sexes and for treatment and surgery in many fields of medicine. In the present study, morphometric characteristics were determined by 3D modelling from computed tomography images obtained from mandibles of Romanov ram and ewe. For this purpose, mandibles of 16 Romanov sheep (eight females and eight males) were used. They were scanned using a 64-detector MDCT device at 80 kV, 200 MA, 639 mGY, and 0.625 mm slice thickness. CT scans were recorded in DICOM format. Reconstructions of the images were made using a special software program. Volume and surface area measurements were made with 22 osteometric parameters of the mandible. GOC-ID had a statistically significant positive correlation with GOC-ID, PC-ID, GOC-MTR, GOC-PTW, GOC-FMN, PMU, MDU, PDU, DU, GOV-PC, GOV-IMD, MTR-MH, MO-MH, FMN- ID, BM, MG, and CG (p < 0.01). GOC-ID had a statistically significant correlation with MTR-ID, GOV-CR, PTW-MH and SI (p < 0.05). When the CR-PC measurement point was examined, it was observed that it had no statistically significant correlation with all measurement points (p > 0.05). As a result of the measurement, it was found that the volume and surface areas were higher in rams than in ewes. The morphometric data obtained would be a reference income in the fields of zoo-archaeology, anatomy, forensics, anaesthesia, surgery, and treatment.

Physical and chemical characterization of femur during and after body development period in male and female rats.

The aim of this study was to determine the physical and chemical characteristics of the bone structures during four developmental periods. Forty Wistar Albino rats (20 male and 20 female) were divided into four groups including prepubertal period (group I), period between adolescence and adulthood (group II) and later (young adult period as group III and old adult period as group IV). The bones were analysed by morphometric measurements, XRF (X-ray fluorescence) analysis for mineral levels and BET analysis (Brunaurer-Emmett-Teller) for surface area and porosity. In morphometric measurements, the GL (greatest length) and the GLC (greatest length from caput femoris) values increased gradually from the first to the fourth group, and these values were higher in the males than the females. Phosphorus and calcium values were higher in the males in adult groups (third and fourth) compared to that for the females; however, they were higher in the females in groups up to adulthood (first and second). While the Ca/P ratio reached the highest value in the second group in the male, it decreased gradually afterwards. In females, the rate, which was close to each other in the first three groups, increased in the fourth group. Surface area size in the female and the male rats was the highest in the second group. In conclusion, changes in the rat bone structure during the development and adulthood periods of the body were revealed, and it was determined that the gender factor was effective in these changes.

A comprehensive assessment, source input determination and distribution of persistent organic pollutants (POPs) along with heavy metals (HMs) in reservoir lake sediments from Çanakkale province, Türkiye.

This study presents the distribution of persistent organic pollutants (POPs), polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) in the sediments from four dams and two ponds in the Çanakkale city, Türkiye. The total concentration varied from 41.47 to 145.45 ng/g d.w. for PAHs; 1.40-14.96 ng/g d.w. for organochlorine pesticides (OCPs) and 0.13-3.31 ng/g d.w. for polychlorinated biphenyls (PCBs). The total polychlorinated dibenzo-p-dioxins/furans (∑PCDD/Fs) concentrations varied between 7.35 and 63.46 pg/g d.w. The HM distribution in sediments ranged from 9.41 to 21.13 mg/kg d.w. for As; 0.20-0.39 mg/kg d.w. for Cd; 19.4-281.68 mg/kg d.w. for Cr; 23.50-118.25 mg/kg d.w. for Pb and 118.9-374.82 mg/kg d.w. for Zn. This paper also elucidates possible sources of POP and HMs in sediment by using diagnostic ratios. The calculated ratios were shown that anthropogenic input is the source of these pollutants in sediments. Principal component analysis showed that the low molecular weight of POPs is considerably related and correlated, while high molecular weight pollutants have made another group and highly correlated with each other. The results indicated that anthropogenic pollution is mainly related to atmospheric input. Anthropogenic pollutants are more apparent at Atikhisar and Bakacak dams close to urbanized locations. Rather than the anthropogenic impact, based on the measured data interpretation, the global climate change effect was seen in Bakacak dam. The overall concentration of all pollutants revealed that Atikhisar and Bakacak sediments are high-level polluted areas according to several types of sediment guidelines.

Morphometric analysis of the skull of Hamdani sheep via Three-Dimensional modelling.

The aim of the present study was to determine the craniometric characteristics of the crania of Hamdani sheep, one of local breeds of Turkey, by using computed tomography (CT). In the study, 13 (six female and seven male) skulls were used. After CT images of the skulls were taken, their three-dimensional models were created by MIMICS 20.1 (The Materialise Group, Leuven, Belgium) program. Thirty-eight craniometric measurements were taken over the models created and nine indices were calculated by using these measurements. All the characteristics examined were expressed as mean ± SE. In the study, statistically significant differences were found between females and males in terms of greatest neurocranium breadth, facial breadth, greatest palatal breadth (p < 0.05) and height of the foramen magnum (p < 0.01) parameters. No statistically significant difference was observed between the sexes in the calculated index values (p > 0.05). In conclusion, it is thought that the findings obtained from the study will contribute to the zooarchaeology and sheep taxonomy of Mesopotamian region.

Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways.

Testicular ischaemia reperfusion (I/R) injury results with serious dysfunctions in testis. This study aims to explore effects of soluble guanylate cyclase (sGC) stimulator Riociguat on experimental testicular I/R injury in rats. Twenty-one male rats were divided into three groups (Control, IR and IRR). The control group was not exposed to any application. Bilateral testis from IR and IRR animals were rotated 720° in opposite directions for 3 h to induce experimental testicular ischaemia. Animals in IR and IRR groups were subjected to 3 h of reperfusion. Isotonic and Riociguat were administered to the animals 30 min prior reperfusion by oral gavage. At the end of experiment, animals were sacrificed and tissue samples were used for analyses. Riociguat treatment significantly decreased tissue malondialdehyde and Luminol levels compared to the IR group (p < 0.05). The pathological changes, pro-apoptotic proteins (Bax, Caspase 3, and Caspase 9) and apoptotic index in the IR group were down regulated in Riociguat treated animals (p < 0.05). Riociguat treatment was also significantly increased anti-apoptotic Bcl-2 expression, but alleviated tissue injury via modulating pro-inflammatory cytokine IL-1ß levels and significantly (p < 0.05) down-regulating NF-κB activity. Moreover, mTOR and ERK phosphorylation increased in IR group (p < 0.05), but Riociguat treatment reduced protein phosphorylation. Our experiment indicated that targeting sGC might support surgical interventions in testicular I/R injury by modulating oxidative stress, inflammation, and apoptotic protein expression levels, but more detailed studies are required to explore the protective activity of Riociguat and underlying mechanisms in testicular I/R injury.