RESUMO
Using indirect immunofluorescence and monoclonal antibodies, we have determined the distribution of Ia+ cells in the non-pregnant rat uterus. In cycling animals, Ia+ cells, most of dendritic shape and non-specific esterase negative, were abundant in the connective tissue of the myometrium and throughout the endometrium, especially adjacent to the luminal and glandular epithelia. In contrast, the number of T lymphocytes was very much less and few of these cells expressed Ia antigen. Stromal Ia+ cells were more numerous during estrus than in any other phase of the cycle. Only a few Ia+ cells were seen in neonatal uterine samples, but the numbers increased to approximately adult levels by 32 days of age. Prepubertal glands were consistently negative, but epithelial expression of Ia antigens in adult uteri showed interesting variations. The luminal epithelium was always negative, whereas the glandular epithelial cells often were positive. Glandular staining was quite heterogeneous in individual samples, but most gland profiles were negative or weakly positive during proestrus and estrus and positive during diestrus. Ovariectomized females given different steroid supplements revealed significant hormonal effects. Estrogen alone dramatically increased the numbers of Ia+ stromal cells compared with control samples, but progesterone alone had no effect. However, progesterone given with estrogen abrogated the estrogenic influence. Progesterone alone caused decreased expression of Ia antigens by the glandular epithelium. If the highly-Ia/ cells in the stroma are capable of antigen presentation, these results indicate a significant potential for local antigen processing with the uterine endometrium.
Assuntos
Antígenos de Histocompatibilidade Classe II/imunologia , Útero/imunologia , Animais , Endométrio/citologia , Endométrio/imunologia , Epitopos/análise , Epitopos/imunologia , Estradiol/farmacologia , Estro/efeitos dos fármacos , Feminino , Imunofluorescência , Antígenos de Histocompatibilidade Classe II/análise , Histocitoquímica , Progesterona/farmacologia , Ratos , Ratos Endogâmicos F344 , Ratos Endogâmicos , Útero/citologiaRESUMO
The initial stages of neovascularization of the corpus luteum were studied in cycling adult rats using light-microscopic autoradiography. The aim of this analysis was to determine whether endothelial mitosis is a factor in this vascular growth and whether there are differences in the amount of mitotic activity in various regions of the ovary. Ovaries were examined at two time intervals: 1-2 hr and 7-8 hr following ovulation. Animals received an intraperitoneal injection of tritiated-thymidine 20 min prior to perfusion fixation of the ovaries. Autoradiographic demonstration of tritiated-thymidine labeling in endothelial nuclei was considered an indication of DNA synthesis preceding mitosis. The percentage of labeled endothelial cells in the ovaries at both time intervals varied according to the region of tissue examined and the stage of differentiation of that region. Stromal vessels were less heavily labeled than thecal vessels. Thecal vessels surrounding growing follicles were more heavily labeled than those surrounding atretic follicles. The heaviest labeling was seen in the developing corpora lutea 7-8 hr following ovulation. Minimal labeling was evident in the corpora lutea which were formed in previous cycles. A regional difference was also detected in the ovarian mesothelium. The portion of the mesothelium overlying ovulated follicles and developing corpora lutea had the greatest percentage of labeled cells. The major findings of this study were: endothelial mitosis was elevated in the initial stages of luteal neovascularization; the heightened endothelial labeling was confined to specific regions of the ovary; and mesothelium in close proximity to the developing corpora lutea also displayed heightened DNA synthesis.