Role of pretreatment and evidence for the enhanced biodegradation and mineralization of low-density polyethylene films by greater waxworm.

The present study reports the role of pretreatment for the enhanced biodegradation of low-density polyethylene (LDPE) with Galleria mellonella (Greater waxworm). The pretreatment of the LDPE film was carried out under solar radiation. The pretreated LDPE (PTLDPE) and untreated LDPE (UTLDPE) were characterized with AFM, FTIR and 1H NMR techniques. The qualitative analysis for the biodegradation of pretreated and untreated LDPE was examined by analysing the Excreta residue (ER) of Galleria mellonella fed with LDPE. The mineralization of the ER of waxworm fed on Waxcomb (WC), UTLDPE and PTLDPE were studied by analysing the changes in physiochemical properties through FTIR, 1H NMR and GC-MS techniques in addition to weight loss percentage of PTLDPE and survival rates of the tested greater waxworms. Solar pretreatment of LDPE led to increased surface roughness which favoured the waxworms to feed voraciously on PTLDPE. The post degradation studies of Waxcomb (WC), PTLDPE and UTLDPE showed 92.03 ± 2.1%, 18.57 ± 1.8% and 55.8 ± 1.2% weight loss, respectively. The FTIR, 1H NMR and GC-MS results confirm that the ER of waxworm fed on WC, UTLDPE and PTLDPE showed the presence of new carbonyl and alcoholic groups with increase in unsaturated hydrocarbon indicating enhanced mineralization of LDPE. The efficient mineralization of PTLDPE by waxworm was observed without affecting its survivability. A plausible mechanism of LDPE degradation has also been proposed. The rapid and cost effective biodegradation of PTLDPE through waxworm paves a new and facile route for hazardous plastic waste treatment.

Efficient biodegradation of polyethylene (HDPE) waste by the plastic-eating lesser waxworm (Achroia grisella).

Polyethylene (PE) is one of the major persistent plastic that is not biodegradable at considerable rates in most environments, and is the major source of unceasing environmental pollution. Recently, biodegradation of plastic wastes through waxworms and mealworms were reported. The present study focuses on the high-density polyethylene (HDPE) degradation capabilities of the larvae of Achroia grisella (lesser waxworm) and its ability to complete its life cycle when fed with HDPE. Effects of added nutrition on PE degradation were assessed, providing wax comb as co-feed (PE-WC). The egested frass of the waxworm fed on waxcomb (WC), PE, and PE-WC were studied by analyzing the changes in physiochemical properties through FTIR and 1H NMR techniques in addition to weight loss percentage of PE and survival rates of the tested lesser waxworms. The post-degradation studies of WC and PE showed 90.5 ± 1.2% and 43.3 ± 1.6% weight loss, respectively, by a group of 100 lesser waxworms. Over an 8-day period, PE consumption increased with an ingestion of 1.83 mg of PE per day per larvae. Supplementing the PE feed of lesser waxworms with WC facilitated enhanced PE degradation showing 69.6 ± 3.2% weight loss. Twenty-eight day survival rates for lesser waxworms fed on WC, PE, and PE-WC were 91.3 ± 1.01%, 74.6 ± 2.9%, and 86 ± 1.4%, respectively. The FTIR and 1H NMR analysis of egested frass indicated formation of new functional organic groups, supporting biodegradation of PE in lesser waxworms. The frass of the lesser waxworm fed on PE samples shows the presence of new carbonyl and alcoholic groups with increase in unsaturated hydrocarbon indicating formation of biodegraded intermediates. Lesser waxworms fed with WC, PE, and PE-WC completed all life cycle stages (larvae, pupae, moth, and egg) developing into a second generation. The second generation of PE-WC fed larvae of A. grisella efficiently degrades PE at par with first generation counterparts.