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Invest Ophthalmol Vis Sci ; 65(5): 4, 2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38691089

RESUMO

Purpose: To investigate the adhesion of Acanthamoeba to scleral contact lens (ScCL) surface according to lens shape. Methods: Two strains of A. polyphaga (CDC:V062 and ATCC 30461) and one clinical Acanthamoeba isolate, were inoculated onto five contact lens (CL): one first-generation silicone hydrogel (SHCL; lotrafilcon B; adhesion control) containing plasma surface treatment; two ScCL (fluorosilicone acrylate) one containing surface treatment composed of plasma and the other containing plasma with Hydra-PEG, and two CL designed with a flat shape having the same material and surface treatments of the ScCL. Trophozoites that adhered to the lens's surfaces were counted by inverted optical light microscopy. Possible alterations of the lens surface that could predispose amoeba adhesion and Acanthamoeba attached to these lens surfaces were evaluated by scanning electron microscopy (SEM). Results: All strains revealed greater adhesion to the ScCL when compared with the flat lenses (P < 0.001). The clinical isolate and the ATCC 30461 had a higher adhesion (P < 0.001) when compared with the CDC:V062. A rough texture was observed on the surface of the lenses that have been examined by SEM. Also, SEM revealed that the isolates had a rounded appearance on the surface of the ScCL in contrast with an elongated appearance on the surface of the silicone hydrogel. Conclusions: The findings revealed that the curved shape of the ScCL favors amoeba adhesion.


Assuntos
Acanthamoeba , Microscopia Eletrônica de Varredura , Acanthamoeba/fisiologia , Acanthamoeba/ultraestrutura , Esclera , Humanos , Lentes de Contato Hidrofílicas/parasitologia , Adesão Celular/fisiologia , Lentes de Contato/parasitologia , Trofozoítos/ultraestrutura , Trofozoítos/fisiologia , Hidrogéis , Animais
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