RESUMO
OBJECTIVE: The objective of the study was to identify factors associated with length of stay (LOS) and 30-day hospital revisit for patients hospitalized with acute pancreatitis (AP). METHOD: Multicenter, retrospective cohort study using the Pediatric Health Information System database. Multilevel linear and logistic regression was used to identify factors independently associated with the primary outcome variables of LOS and 30-day hospital revisit in children aged 1 and 18 years discharged with a primary discharge diagnosis of AP from participating hospitals between 2008 and 2013. RESULTS: For the 7693 discharges, median LOS was 4 days (interquartile range 3-7 days) and 30-day revisit rate 17.6% (nâ=â1356). Discharges were primarily girls (55%), Caucasian (46%), and 6 years old or older (85%). On multilevel regression, factors independently associated with both longer LOS and higher revisit odds included malignant and gastrointestinal complex chronic conditions (CCCs) and total parenteral nutrition use while hospitalized. Male gender was associated with both lower LOS (adjusted length of stayâ=â-0.6 days, 95% confidence interval [CI]â=â-0.8 to -0.4) and decreased revisit odds (aOR 0.85; 95% CIâ=â0.74 to 0.97). Hispanic ethnicity was associated with increased LOS (adjusted length of stayâ=â+0.8 days, 95% CIâ=â+0.5 to +1.1), but no change in revisit odds. CONCLUSIONS: Certain demographic and clinical factors, including gender, ethnicity, and type of CCC, were independently associated with LOS and risk of 30-day hospital revisit for pediatric AP. Children with malignant and gastrointestinal CCCs who require total parenteral nutrition are at highest risk for both longer LOS and hospital revisit when admitted with AP. These patient populations may benefit from intensive care coordination when hospitalized for AP.
Assuntos
Criança Hospitalizada , Tempo de Internação , Avaliação de Resultados em Cuidados de Saúde , Pancreatite/terapia , Readmissão do Paciente , Doença Aguda , Adolescente , California , Criança , Serviços de Saúde da Criança , Pré-Escolar , Estudos de Coortes , Bases de Dados Factuais , Feminino , Hospitais Pediátricos , Humanos , Lactente , Modelos Lineares , Masculino , Estudos Retrospectivos , Fatores de RiscoAssuntos
Mitocôndrias , Saccharomycetales/ultraestrutura , Coloração e Rotulagem/métodos , Algoritmos , Parede Celular/química , Corantes Fluorescentes/análise , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica/métodos , Potencial da Membrana Mitocondrial , Mitocôndrias/química , Mitocôndrias/metabolismo , Modelos Biológicos , Reação em Cadeia da Polimerase/métodos , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Saccharomycetales/genética , Saccharomycetales/crescimento & desenvolvimento , Transformação GenéticaRESUMO
The budding yeast Saccharomyces cerevisiae has many advantages as a model system, but until recently high-resolution microscopy was not often attempted in this organism. Its small size, rounded shape, and rigid cell wall were obstacles to exploring the cell biology of this model eukaryote. However, it is now feasible for laboratories to acquire and analyze high-resolution, multidimensional images of yeast cell biology, including the mitochondria. As a result, imaging of yeast has emerged as an important tool in eukaryotic cell biology. This chapter describes labeling methods and optical approaches for visualizing yeast mitochondria using fluorescence microscopy.