[Absorption spectrum study of HeLa cells treated with vacuum and low-energy ions implantation].

Mineral oil was selected to protect HeLa cells from water evaporation during low-energy ions implantation in the present paper. Then, HeLa cells having been treated with vacuum and low-energy N+ ions implantation were used to collect ultraviolet absorption spectrum by spectrophotometer. Analytical results indicated that HeLa cells had some characteristic absorption peaks near 202 and 260 nm, respectively. And then the study also found: (1) The spectral intensity increased with the vacuum treatment time. In addition, the effect of vacuum on cellular spectrum was greater than that of mineral oil. (2) The influence of low energy N+ ions on absorption spectrum was far more than that of vacuum. (3) The spectral intensity increased with the implantation dose. According to these results, the effect of low-energy N+ ions implantation and vacuum on tumorous cells (HeLa cells), especially on the molecular configuration and component of tumorous cells (HeLa cells) was discussed. In a word, this study provides a basis for further research on the functionary mechanism of low-energy ions implantation on biomaterial.

[Study the Raman spectroscopy of breast tumor limbic tissue].

The confocal Raman micro-spectra of breast tumor limbic fat tissues of forty cases after surgery were collected. Substantial differences were found among breast infiltrative ductal carcinoma, hyperplasia and fibroadenoma, which is possibly to be used as a character of breast cancer and other breast tumor and offers the evidence for the breast tumor diagnosis. So, the Raman spectroscopy of the breast limbic fat tissue is of great value.

[Study on absorption spectra of malignant plasma of esophagus].

There are too many articles about diagnosing cancers by the content of tumor chemical signs in plasma, but there are few articles about diagnosing them by spectroscopy. The present article belongs to the few. The authors can differentiate the normal and malignant esophagus by the statistic and analysis of absorption spectra after their plasmas are detected by spectrophotometry, which provide a new approach to quickly diagnosing malignant esophagus.

[Fuzzing pattern recognition study on Raman spectrum of tumor peripheral tissue].

On the basis of some theories about fuzzing pattern recognition, the present article studied the data preprocessing of the Raman spectrum of tumor peripheral tissue, and feature extraction and selection. According to these features the authors improved the leaning towards the bigger membership function of trapezoidal distribution. The authors built the membership function of Raman spectrum of tumor peripheral tissue which belongs to malignant tumor on the basis of 40 specimens, and designed the classifier. The test of other 40 specimens showed that the discrimination of malignant tumor is 82.4%, while that of beginning tumor is 73.9%.

[Effect of cancer cells on the spectra of serums].

This article studied the different effects on the spectra of serums by adding different amount of cancer cells into serum, and gives the comparison between CNE cells' serum spectra and Hela' as well as that between abnormal serums and normal ones. Experiment results showed that the intensity of the absorption spectra and the fluorescence spectra exhibits a trend that it increases with the decrease in the cancer cells' number, while begins to drop when it goes up to a certain extent. The intensity of the normal serums' spectra is obviously higher than that of the abnormal one. It is concluded that the changes in the intensity of fluorescence spectra' show that the cancer cells restrain the vibration of some group in the normal serums, and the absorption spectra' reveal that the cancer cells prevent the casein from absorbing light.

[The study of absorption spectrum for cell substrate].

The authors collected the absorption spectrum of RPMI 1640 and DMEM substrates that cultivated Hela and CNE by UV-3101 spectrophotometer and analysed the absorbability of proteins in the substrate. The absorption peaks of the RPMI 1 640 culture medium that cultivated cells for different times shifted from 227 to 222 or 218 nm and from 278 to 280 nm respectively; while during growing course of cultivated cells, one of the absorption peaks of DMEM culture medium shifted from 224 nm to one near 221 nm, and the absorption peak 278 nm almost had no shift. All of these shifts show that the content of each amino acid such as tryptophan and casein has already changed. That is, during the growing course of cultivating cancer cells, the tryptophan and casein were not depleted equivalently. In the growth period of Hela and CNE, they consumed different amino acid. So they need different component proportion for amino acid.