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It is known that anthropometric data (weight, height, BMI, waist circumference and WHtR) and male gender are positively correlated with greater core strength, while age is negatively correlated. For competitive athletes with no significant differences in the anthropometric data stated above, there have hardly been any studies in which isometric core strength in a seated position is quantitatively compared among athletes in different sports. This study aimed to analyse different sports in well-trained athletes using military competitive sports as an example with regard to possible differences in core strength. For this purpose, Parachuting (n=28), Military Pentathlon (n=34) and Ski Platoon (n=38) groups were compared with regard to isokinetic core strength using dynamometry in seated position. In addition to the comparison of anthropometry and isokinetic results with descriptive statistics, ANOVA and Welch-ANOVA were used to compare the means of absolute and weight-adjusted core strength. It was shown that the Ski Platoon group had significantly higher flexion peak torque values than the Military Pentathlon and Parachuting groups. Even when adjusted for weight, the values were significantly higher than those measured in the Military Pentathlon group but not significantly higher than those in the Parachuting group. For extension peak torque, it was found that the Military Pentathlon group had significantly lower values than the Ski Platoon group and, in the weight-adjusted analysis, significantly lower values than the Parachuters. Using the example of competitive military sports, this study was able to show that there are significant differences in isokinetic core strength even among professional competitive athletes.This knowledge should be used to carry out detailed quantitative analyses of core strength, even in well-trained professional athletes, to find applications for prevention or to coordinate compensating exercises.
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Militares , Força Muscular , Humanos , Masculino , Força Muscular/fisiologia , Adulto , Feminino , Adulto Jovem , Aviação , Doenças Profissionais/fisiopatologia , Atletas , Esqui/fisiologia , Fenômenos BiomecânicosRESUMO
In this work, we propose a novel single-end morphing capacitive sensing method for shape tracking, FxC, by combining Folding origami structures and Capacitive sensing to detect the morphing structural motions using state-of-the-art sensing circuits and deep learning. It was observed through embedding areas of origami structures with conductive materials as single-end capacitive sensing patches, that the sensor signals change coherently with the motion of the structure. Different from other origami capacitors where the origami structures are used in adjusting the thickness of the dielectric layer of double-plate capacitors, FxC uses only a single conductive plate per channel, and the origami structure directly changes the geometry of the conductive plate. We examined the operation principle of morphing single-end capacitors through 3D geometry simulation combined with physics theoretical deduction, which deduced similar behavior as observed in experimentation. Then a software pipeline was developed to use the sensor signals to reconstruct the dynamic structural geometry through data-driven deep neural network regression of geometric primitives extracted from vision tracking. We created multiple folding patterns to validate our approach, based on folding patterns including Accordion, Chevron, Sunray and V-Fold patterns with different layouts of capacitive sensors using paper-based and textile-based materials. Experimentation results show that the geometry primitives predicted from the capacitive signals have a strong correlation with the visual ground truth with R-squared value of up to 95% and tracking error of 6.5 mm for patches. The simulation and machine learning constitute two-way information exchange between the sensing signals and structural geometry. By embedding part of the origami surface with morphing single-end capacitive sensors, FxC presents a unique solution that leverages both the mechanical properties of origami and sensing properties of capacitive sensing.
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This work presents a novel and versatile approach to employ textile capacitive sensing as an effective solution for capturing human body movement through fashionable and everyday-life garments. Conductive textile patches are utilized for sensing the movement, working without the need for strain or direct body contact, wherefore the patches can sense only from their deformation within the garment. This principle allows the sensing area to be decoupled from the wearer's body for improved wearing comfort and more pleasant integration. We demonstrate our technology based on multiple prototypes which have been developed by an interdisciplinary team of electrical engineers, computer scientists, digital artists, and smart fashion designers through several iterations to seamlessly incorporate the technology of capacitive sensing with corresponding design considerations into textile materials. The resulting accumulation of textile capacitive sensing wearables showcases the versatile application possibilities of our technology from single-joint angle measurements towards multi-joint body part tracking.
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Movimento , Têxteis , Dispositivos Eletrônicos Vestíveis , Humanos , Capacitância Elétrica , Desenho de EquipamentoRESUMO
Heterogeneous sandwich immunoassays are widely used for biomarker detection in bioanalysis and medical diagnostics. The high analyte sensitivity of the current "gold standard" enzyme-linked immunosorbent assay (ELISA) originates from the signal-generating enzymatic amplification step, yielding a high number of optically detectable reporter molecules. For future point-of-care testing (POCT) and point-of-need applications, there is an increasing interest in more simple detection strategies that circumvent time-consuming and temperature-dependent enzymatic reactions. A common concept to aim for detection limits comparable to those of enzymatic amplification reactions is the usage of polymer nanoparticles (NP) stained with a large number of chromophores. We explored different simple NP-based signal amplification strategies for heterogeneous sandwich immunoassays that rely on an extraction-triggered release step of different types of optically detectable reporters. Therefore, streptavidin-functionalized polystyrene particles (PSP) are utilized as carriers for (i) the fluorescent dye coumarin 153 (C153) and (ii) hemin (hem) molecules catalyzing the luminol reaction enabling chemiluminescence (CL) detection. Additionally, (iii) NP labeling with hemin-based microperoxidase MP11 was assessed. For each amplification approach, the PSP was first systematically optimized regarding size, loading concentration, and surface chemistry. Then, for an immunoassay for the inflammation marker C-reactive protein (CRP), the analyte sensitivity achievable with optimized PSP systems was compared with the established ELISA concept for photometric and CL detection. Careful optimization led to a limit of detection (LOD) of 0.1 ng/mL for MP11-labeled PSP and CL detection, performing similarly well to a photometric ELISA (0.13 ng/mL), which demonstrates the huge potential of our novel assay concept.
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Hemina , Nanopartículas , Imunoensaio , Ensaio de Imunoadsorção Enzimática , Nanopartículas/química , BiomarcadoresRESUMO
Surface-functionalized polymer beads encoded with molecular luminophores and nanocrystalline emitters such as semiconductor nanocrystals, often referred to as quantum dots (QDs), or magnetic nanoparticles are broadly used in the life sciences as reporters and carrier beads. Many of these applications require a profound knowledge of the chemical nature and total number of their surface functional groups (FGs), that control bead charge, colloidal stability, hydrophobicity, and the interaction with the environment and biological systems. For bioanalytical applications, also the number of groups accessible for the subsequent functionalization with, e.g., biomolecules or targeting ligands is relevant. In this study, we explore the influence of QD encoding on the amount of carboxylic acid (COOH) surface FGs of 2 µm polystyrene microparticles (PSMPs). This is done for frequently employed oleic acid and oleylamine stabilized, luminescent core/shell CdSe QDs and two commonly used encoding procedures. This included QD addition during bead formation by a thermally induced polymerization reaction and a post synthetic swelling procedure. The accessible number of COOH groups on the surface of QD-encoded and pristine beads was quantified by two colorimetric assays, utilizing differently sized reporters and electrostatic and covalent interactions. The results were compared to the total number of FGs obtained by a conductometric titration and Fourier transform infrared spectroscopy (FTIR). In addition, a comparison of the impact of QD and dye encoding on the bead surface chemistry was performed. Our results demonstrate the influence of QD encoding and the QD-encoding strategy on the number of surface FG that is ascribed to an interaction of the QDs with the carboxylic acid groups on the bead surface. These findings are of considerable relevance for applications of nanoparticle-encoded beads and safe-by-design concepts for nanomaterials.
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Outer membrane lipopolysaccharides (LPS) play a crucial role in determining attachment behavior and pathogenicity of bacteria. The aim of this study was to develop a simple procedure for anchoring bacterial lipopolysaccharides to polystyrene (PS) microparticles as a model system for in situ attachment studies. By using a swell-capture methodology, commercially available LPS of Pseudomonas aeruginosa (strain ATCC 27316 serotype 10.22) was anchored onto PS microparticles in a proof-of-concept study. A detailed chemical and morphological characterization has proven the success of LPS incorporation. It was shown that the coverage and structure of the LPS film was concentration dependent. The procedure can easily be adapted to LPS of other bacterial strains to generate a synthetic model toolkit for attachment studies.
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Lipopolissacarídeos , Poliestirenos , Lipopolissacarídeos/metabolismo , Poliestirenos/metabolismo , Virulência , Pseudomonas aeruginosa/metabolismoRESUMO
The relationship between trunk strength and athletic performance is well known. In the past, trunk strength and athletic performance were measured in field tests. Previous studies encouraged sport-specific analyses. The goal of this study was to investigate whether there is a relation between ergometrically measured treadmill or bicycle endurance and isokinetic trunk strength. This retrospective analysis included 1334 bicycle and 1838 treadmill ergometry examinations in 1149 subjects. Bicycle and treadmill ergometer performance were analysed in relation to isokinetic trunk strength. Statistics were performed by Pearson correlation and mixed or generalised linear models. Higher treadmill and bicycle power correlated with higher isokinetic trunk strength, with highest absolute trunk strength in the treadmill group. For both running and cycling endurance, a positive correlation with trunk strength could be quantified in regression models. Increased ergometry endurance and lower flexion/extension ratios are connected weakly. Ergometry performance had the strongest correlation with extension trunk strength (r=0.312-0.398 for bicycle ergometry and r=0.168-0.229 for treadmill ergometry, p<0.001). We encourage prospective studies using both kinds of ergometry to evaluate the effect of trunk strengthening to enhance sport-specific endurance performance. Weight-adapted trunk strength values showed overall greater correlation to trunk strength and we recommend the use of weight-adapted values.
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Desempenho Atlético , Ciclismo , Humanos , Estudos Prospectivos , Estudos Retrospectivos , Teste de Esforço , ErgometriaRESUMO
Functional nanomaterials (NM) of different size, shape, chemical composition, and surface chemistry are of increasing relevance for many key technologies of the twenty-first century. This includes polymer and silica or silica-coated nanoparticles (NP) with covalently bound surface groups, semiconductor quantum dots (QD), metal and metal oxide NP, and lanthanide-based NP with coordinatively or electrostatically bound ligands, as well as surface-coated nanostructures like micellar encapsulated NP. The surface chemistry can significantly affect the physicochemical properties of NM, their charge, their processability and performance, as well as their impact on human health and the environment. Thus, analytical methods for the characterization of NM surface chemistry regarding chemical identification, quantification, and accessibility of functional groups (FG) and surface ligands bearing such FG are of increasing importance for quality control of NM synthesis up to nanosafety. Here, we provide an overview of analytical methods for FG analysis and quantification with special emphasis on bioanalytically relevant FG broadly utilized for the covalent attachment of biomolecules like proteins, peptides, and oligonucleotides and address method- and material-related challenges and limitations. Analytical techniques reviewed include electrochemical titration methods, optical assays, nuclear magnetic resonance and vibrational spectroscopy, as well as X-ray based and thermal analysis methods, covering the last 5-10 years. Criteria for method classification and evaluation include the need for a signal-generating label, provision of either the total or derivatizable number of FG, need for expensive instrumentation, and suitability for process and production control during NM synthesis and functionalization.
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Técnicas de Química Analítica/métodos , Nanopartículas Metálicas/análise , Nanopartículas Metálicas/química , Corantes Fluorescentes/química , Ligantes , Metais Pesados/químicaRESUMO
Controlling thickness and tightness of surface passivation shells is crucial for many applications of core-shell nanoparticles (NP). Usually, to determine shell thickness, core and core/shell particle are measured individually requiring the availability of both nanoobjects. This is often not fulfilled for functional nanomaterials such as many photoluminescent semiconductor quantum dots (QD) used for bioimaging, solid state lighting, and display technologies as the core does not show the application-relevant functionality like a high photoluminescence (PL) quantum yield, calling for a whole nanoobject approach. By combining high-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), a novel whole nanoobject approach is developed representatively for an ultrabright oleic acid-stabilized, thick shell CdSe/CdS QD with a PL quantum yield close to unity. The size of this spectroscopically assessed QD, is in the range of the information depth of usual laboratory XPS. Information on particle size and monodispersity were validated with dynamic light scattering (DLS) and small angle X-ray scattering (SAXS) and compared to data derived from optical measurements. In addition to demonstrating the potential of this novel whole nanoobject approach for determining architectures of small nanoparticles, the presented results also highlight challenges faced by different sizing and structural analysis methods and method-inherent uncertainties.
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Organic and inorganic nanoparticles (NPs) are increasingly used as drug carriers, fluorescent sensors, and multimodal labels in the life and material sciences. These applications require knowledge of the chemical nature, total number of surface groups, and the number of groups accessible for subsequent coupling of e.g., antifouling ligands, targeting bioligands, or sensor molecules. To establish the concept of catch-and-release assays, cleavable probes were rationally designed from a quantitatively cleavable disulfide moiety and the optically detectable reporter 2-thiopyridone (2-TP). For quantifying surface groups on nanomaterials, first, a set of monodisperse carboxy-and amino-functionalized, 100 nm-sized polymer and silica NPs with different surface group densities was synthesized. Subsequently, the accessible functional groups (FGs) were quantified via optical spectroscopy of the cleaved off reporter after its release in solution. Method validation was done with inductively coupled plasma optical emission spectroscopy (ICP-OES) utilizing the sulfur atom of the cleavable probe. This comparison underlined the reliability and versatility of our probes, which can be used for surface group quantification on all types of transparent, scattering, absorbing and/or fluorescent particles. The correlation between the total and accessible number of FGs quantified by conductometric titration, qNMR, and with our cleavable probes, together with the comparison to results of conjugation studies with differently sized biomolecules reveal the potential of catch-and-release reporters for surface analysis. Our findings also underline the importance of quantifying particularly the accessible amount of FGs for many applications of NPs in the life sciences.
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Many applications of nanometer- and micrometer-sized particles include their surface functionalization with linkers, sensor molecules, and analyte recognition moieties like (bio)ligands. This requires knowledge of the chemical nature and number of surface groups accessible for subsequent coupling reactions. Particularly attractive for the quantification of these groups are spectrophotometric and fluorometric assays, which can be read out with simple instrumentation. In this respect, we present here a novel family of cleavable spectrophotometric and multimodal reporters for conjugatable amino and carboxyl surface groups on nano- and microparticles. This allows determination of particle-bound labels, unbound reporters in the supernatant, and reporters cleaved off from the particle surface, as well as the remaining thiol groups on particle, by spectrophotometry and inductively coupled optical emission spectrometry (32S ICP-OES). Comparison of the performance of these cleavable reporters with conductometry and conventional labels, utilizing changes in intensity or color of absorption or emission, underlines the analytical potential of this versatile concept which elegantly circumvents signal distortions by scattering and encoding dyes and enables straightforward validation by method comparison.
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The exceptional photophysical properties and the nanometric dimensions of colloidal semiconductor quantum dots (QD) have strongly attracted the bioanalytical community over the last approximately 20 y. In particular, the integration of QDs in the analysis of biological components and interactions, and the related diagnostics using Förster resonance energy transfer (FRET), have allowed researchers to significantly improve and diversify fluorescence-based biosensing. In this TRENDS article, we review some recent developments in QD-FRET biosensing that have implemented this technology in electronic consumer products, multiplexed analysis, and detection without light excitation for diagnostic applications. In selected examples of smartphone-based imaging, single- and multistep FRET, steady-state and time-resolved spectroscopy, and bio/chemiluminescence detection of QDs used as both FRET donors and acceptors, we highlight the advantages of QD-based FRET biosensing for multiplexed and sensitive diagnostics. Graphical Abstract Quantum dots (QDs) can be applied as donors and/or acceptors for Förster resonance energy transfer- (FRET-) based biosensing for multiplexed and sensitive diagnostics in various assay formats.
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Técnicas de Laboratório Clínico/métodos , Transferência Ressonante de Energia de Fluorescência/métodos , Pontos Quânticos , Técnicas BiossensoriaisRESUMO
This paper describes the production and characteristics of the nanoparticle test materials prepared for common use in the collaborative research project NanoChOp (Chemical and optical characterization of nanomaterials in biological systems), in casu suspensions of silica nanoparticles and CdSe/CdS/ZnS quantum dots (QDs). This paper is the first to illustrate how to assess whether nanoparticle test materials meet the requirements of a "reference material" (ISO Guide 30, 2015) or rather those of the recently defined category of "representative test material (RTM)" (ISO/TS 16195, 2013). The NanoChOp test materials were investigated with small-angle X-ray scattering (SAXS), dynamic light scattering (DLS), and centrifugal liquid sedimentation (CLS) to establish whether they complied with the required monomodal particle size distribution. The presence of impurities, aggregates, agglomerates, and viable microorganisms in the suspensions was investigated with DLS, CLS, optical and electron microscopy and via plating on nutrient agar. Suitability of surface functionalization was investigated with attenuated total reflection Fourier transform infrared spectrometry (ATR-FTIR) and via the capacity of the nanoparticles to be fluorescently labeled or to bind antibodies. Between-unit homogeneity and stability were investigated in terms of particle size and zeta potential. This paper shows that only based on the outcome of a detailed characterization process one can raise the status of a test material to RTM or reference material, and how this status depends on its intended use.
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The importance of microRNA (miRNA) dysregulation for the development and progression of diseases and the discovery of stable miRNAs in peripheral blood have made these short-sequence nucleic acids next-generation biomarkers. Here we present a fully homogeneous multiplexed miRNA FRET assay that combines careful biophotonic design with various RNA hybridization and ligation steps. The single-step, single-temperature, and amplification-free assay provides a unique combination of performance parameters compared to state-of-the-art miRNA detection technologies. Precise multiplexed quantification of miRNA-20a, -20b, and -21 at concentrations between 0.05 and 0.5â nM in a single 150â µL sample and detection limits between 0.2 and 0.9â nM in 7.5â µL serum samples demonstrate the feasibility of both high-throughput and point-of-care clinical diagnostics.
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Transferência Ressonante de Energia de Fluorescência , Fluorescência , MicroRNAs/análiseRESUMO
Luminescent lanthanide labels (LLLs) and semiconductor quantum dots (QDs) are two very special classes of (at least partially) inorganic fluorophores, which provide unique properties for Förster resonance energy transfer (FRET). FRET is an energy-transfer process between an excited donor fluorophore and a ground-state acceptor fluorophore in close proximity (approximately 1-20 nm), and therefore it is extremely well suited for biosensing applications in optical spectroscopy and microscopy. Within this cogent review, we will outline the main photophysical advantages of LLLs and QDs and their special properties for FRET. We will then focus on some recent applications from the FRET biosensing literature using LLLs as donors and QDs as donors and acceptors in combination with several other fluorophores. Recent examples of combining LLLs and QDs for spectral and temporal multiplexing from single-step to multistep FRET demonstrate the versatile and powerful biosensing capabilities of this unique FRET pair. As this review is published in the Forum on Imaging and Sensing, we will also present some new results of our groups concerning LLL-based time-gated cellular imaging with optically trifunctional antibodies and LLL-to-QD FRET-based homogeneous sandwich immunoassays for the detection of carcinoembryonic antigen.
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Transferência Ressonante de Energia de Fluorescência , Elementos da Série dos Lantanídeos/química , Imagem Molecular/métodos , Pontos QuânticosRESUMO
Simultaneous monitoring of multiple molecular interactions and multiplexed detection of several diagnostic biomarkers at very low concentrations have become important issues in advanced biological and chemical sensing. Here we present an optically multiplexed six-color Förster resonance energy transfer (FRET) biosensor for simultaneous monitoring of five different individual binding events. We combined simultaneous FRET from one Tb complex to five different organic dyes measured in a filter-based time-resolved detection format with a sophisticated spectral crosstalk correction, which results in very efficient background suppression. The advantages and robustness of the multiplexed FRET sensor were exemplified by analyzing a 15-component lung cancer immunoassay involving 10 different antibodies and five different tumor markers in a single 50 µL human serum sample. The multiplexed biosensor offers clinically relevant detection limits in the low picomolar (ng/mL) concentration range for all five markers, thus providing an effective early screening tool for lung cancer with the possibility of distinguishing small-cell from non-small-cell lung carcinoma. This novel technology will open new doors for multiple biomarker diagnostics as well as multiplexed real-time imaging and spectroscopy.
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Biomarcadores Tumorais/sangue , Técnicas Biossensoriais , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Cor , Transferência Ressonante de Energia de Fluorescência , Neoplasias Pulmonares/diagnóstico , Carcinoma de Pequenas Células do Pulmão/diagnóstico , Corantes Fluorescentes/química , Humanos , Térbio/química , Fatores de TempoRESUMO
Applications based on Förster resonance energy transfer (FRET) play an important role for the determination of concentrations and distances within nanometer-scale systems in vitro and in vivo in many fields of biotechnology. Semiconductor nanocrystals (Quantum dots - QDs) possess ideal properties for their application as FRET acceptors when the donors have long excited state lifetimes and when direct excitation of QDs can be efficiently suppressed. Therefore, luminescent terbium complexes (LTCs) with excited state lifetimes of more than 2 ms are ideal FRET donor candidates for QD-acceptors. This chapter will give a short overview of theoretical and practical background of FRET, QDs and LTCs, and present some recent applications of LTC-QD FRET pairs for multiplexed ultra-sensitive in vitro diagnostics and nanometer-resolution molecular distance measurements.
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Técnicas Biossensoriais , Transferência Ressonante de Energia de Fluorescência/métodos , Imagem Molecular , Pontos Quânticos , Semicondutores , Imunoensaio , Substâncias Luminescentes/química , Técnicas de Diagnóstico Molecular , Térbio/químicaRESUMO
Förster resonance energy transfer (FRET) from luminescent terbium complexes (LTC) as donors to semiconductor quantum dots (QDs) as acceptors allows extraordinary large FRET efficiencies due to the long Förster distances afforded. Moreover, time-gated detection permits an efficient suppression of autofluorescent background leading to sub-picomolar detection limits even within multiplexed detection formats. These characteristics make FRET-systems with LTC and QDs excellent candidates for clinical diagnostics. So far, such proofs of principle for highly sensitive multiplexed biosensing have only been performed under optimized buffer conditions and interactions between real-life clinical media such as human serum or plasma and LTC-QD-FRET-systems have not yet been taken into account. Here we present an extensive spectroscopic analysis of absorption, excitation and emission spectra along with the luminescence decay times of both the single components as well as the assembled FRET-systems in TRIS-buffer, TRIS-buffer with 2% bovine serum albumin, and fresh human plasma. Moreover, we evaluated homogeneous LTC-QD FRET assays in QD conjugates assembled with either the well-known, specific biotin-streptavidin biological interaction or, alternatively, the metal-affinity coordination of histidine to zinc. In the case of conjugates assembled with biotin-streptavidin no significant interference with the optical and binding properties occurs whereas the histidine-zinc system appears to be affected by human plasma.