Effect of biochar on microplastics penetration treatment within soil porous medium under the wetting-drying cycles and optimisation of soil-biochar mixing format.

Plant-based biochar was demonstrated promising capability in adsorbing microplastic particles (MPs) within soil porous mediums. However, biochar's function in mitigating MPs' vertical penetration during wetting-drying cycles, typical of seasonal precipitation and evaporation, remains uncertain. Furthermore, few studies have investigated the structures of how biochar combines with soil. This study conducted column tests to assess the MPs retention capabilities of soil-biochar porous media under saturated and wetting-drying conditions. The water retention and hydrophilic properties were investigated to elucidate the impact of wetting-drying cycles. Additionally, different biochar-soil structures were compared to optimise the structural design. Without biochar, wetting-drying cycles resulted in 8.74 % more MPs escaping from samples. However, incorporating 15 % biochar led to only around 2 % more MPs in effluent. Biochar significantly enhanced soil's MP absorption capacity and mitigated the negative effects of wetting-drying cycles. Biochar's alveolate morphology provides ample adsorption sites and creates complex flow paths. The hydrophilic groups of biochar and capillarity by micropores facilitated slower water release during drying, preventing crack propagation and flush on MP particles. This effect was more pronounced with higher biochar content and lower porosity. Moreover, layer structure was found to improve MPs removal, benefiting the long-term performance and management of the biochar functional layer.

FSH Is Responsible for Androgen Deprivation Therapy-Associated Atherosclerosis in Mice by Exaggerating Endothelial Inflammation and Monocyte Adhesion.

BACKGROUND: Androgen deprivation therapy (ADT) is the mainstay treatment for advanced prostate cancer. But ADTs with orchiectomy and gonadotropin-releasing hormone (GnRH) agonist are associated with increased risk of cardiovascular diseases, which appears less significant with GnRH antagonist. The difference of follicle-stimulating hormone (FSH) in ADT modalities is hypothesized to be responsible for ADT-associated cardiovascular diseases. METHODS: We administered orchiectomy, GnRH agonist, or GnRH antagonist in male ApoE-/- mice fed with Western diet and manipulated FSH levels by testosterone and FSH supplementation or FSH antibody to investigate the role of FSH elevation on atherosclerosis. By combining lipidomics, in vitro study, and intraluminal FSHR (FSH receptor) inhibition, we delineated the effects of FSH on endothelium and monocytes and the underlying mechanisms. RESULTS: Orchiectomy and GnRH agonist, but not GnRH antagonist, induced long- or short-term FSH elevation and significantly accelerated atherogenesis. In orchiectomized and testosterone-supplemented mice, FSH exposure increased atherosclerosis. In GnRH agonist-treated mice, blocking of short FSH surge by anti-FSHß antibody greatly alleviated endothelial inflammation and delayed atherogenesis. In GnRH antagonist-treated mice, FSH supplementation aggravated atherogenesis. Mechanistically, FSH, synergizing with TNF-α (tumor necrosis factor alpha), exacerbated endothelial inflammation by elevating VCAM-1 (vascular cell adhesion protein 1) expression through the cAMP/PKA (protein kinase A)/CREB (cAMP response element-binding protein)/c-Jun and PI3K (phosphatidylinositol 3 kinase)/AKT (protein kinase B)/GSK-3ß (glycogen synthase kinase 3 beta)/GATA-6 (GATA-binding protein 6) pathways. In monocytes, FSH upregulated CD29 (cluster of differentiation 29) expression via the PI3K/AKT/GSK-3ß/SP1 (specificity protein 1) pathway and promoted monocyte-endothelial adhesion both in vitro and in vivo. Importantly, FSHR knockdown by shRNA in endothelium of carotid arteries markedly reduced GnRH agonist-induced endothelial inflammation and atherosclerosis in mice. CONCLUSIONS: FSH is responsible for ADT-associated atherosclerosis by exaggerating endothelial inflammation and promoting monocyte-endothelial adhesion.

Molecular Dynamics Study on the Adsorption and Modification Mechanism of Polymeric Sand-Fixing Agent.

Chemical sand-fixing technology has shown good potential in preventing desertification, but the effect is determined by materials. In this paper, the adsorption behavior of quartz and six common polymer sand-fixing agents under dry conditions was studied by molecular dynamics method. The results show that all polymers could be adsorbed on the surface of quartz and their functional groups play an important role in the adsorption process. Compared with other materials, the binding energy and the number of hydrogen bonds of PAA-quartz composites were improved by 30.7-65.6% and 8.3-333.3%, respectively. It was found that the number of hydrogen bonds formed under the unit molecular was positively correlated with the mechanical properties of the improved sandy soil. This study provides an accurate, efficient and inexpensive qualitative evaluation method for the curing effect of sand fixers, which will assist in the screening and development of new high performance sand fixers.

A hybrid machine-learning model to map glacier-related debris flow susceptibility along Gyirong Zangbo watershed under the changing climate.

Gyirong serves as an important channel to Chine-Nepal Economic Corridor, which is also the only land route for China-Nepal trade since the 2015 earthquake. However, the Gyirong corridor suffers from glacier-related debris flow from every April to September because of the complex topographic features and the changing climate. Therefore, a susceptibility map in response to precipitation and temperature change is timely, not only to ensure the safe operation of this corridor, but also to provide decision-makers a guidance for hazard mitigation and environmental remediation. Conventional method is difficult to consider and link the meteorological factors (e.g. temperature and precipitation), topographies, ecological, geological conditions all together to produce the susceptibility map, as such, machine learning is utilised to conduct the analysis. Logistic Regression (LR) and Support Vector Machine (SVM) were firstly applied to evaluate their efficiency and effectiveness of the performance of producing the susceptibility map. In order to improve the fitting and prediction accuracy (ACC), genetic algorithm - support vector machine (GA-SVM) and certainty factor - genetic algorithm - support vector machine (CF-GA-SVM) were conducted based on the initial analysis results of receiver operating characteristics curve (ROC) and ACC. Through the analysis, it can be seen that over 61% of the study areas have a high susceptibility to debris flow, requiring an intensive attention from the local government. To further optimise the computational time, when dealing with small amounts of sample data, SVM is more efficient than LR, but CF-GA-SVM can achieve the highest AUC (Area Under Curve) and ACC values, 0.945 and 0.800, respectively. Overall, CF-GA-SVM model presents a relatively high robustness according to sensitivity analysis.

On-site CO2 bio-sequestration in anaerobic digestion: Current status and prospects.

The advantages of anaerobic digestion (AD) technology in organic solid waste treatment for bioenergy recovery are evidenced in worldwide. Recently, more attention has been paid to on-site biogas research, as well as biogenic CO2 sequestration from AD plant, to promote "carbon neutral". Single-phase and two-phase AD system can be incorporated with various CO2 bioconversion technologies through H2 mediated CO2 bioconversion (in-situ and ex-situ biogas upgrading), or other emerging strategies for CO2 fixation without exogenous H2 injection; these include in-situ direct interspecies electron transfer reinforcement, electromethanogenesis, and off-gas reutilization. The existing and potential scenarios for on-site CO2 bio-sequestration within the AD framework are reviewed from the perspectives of metabolic pathways, functional microorganisms, the limitations on reaction kinetics. This review concluded that on-site CO2 bio-sequestration is a promising solution to reduce greenhouse gas emissions and increase renewable energy recovery.

Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions.

Dendritic cells (DCs) are the most potent antigen-presenting cells. Upon maturation, DCs express costimulatory molecules and migrate to the lymph nodes to present antigens to T cells. The actin cytoskeleton plays key roles in multiple aspects of DC functions. However, little is known about the mechanisms and identities of actin-binding proteins that control DC maturation and maturation-associated functional changes. Tropomodulin1 (Tmod1), an actin-capping protein, controls actin depolymerization and nucleation. We found that Tmod1 was expressed in bone marrow-derived immature DCs and was significantly upregulated upon lipopolysaccharide (LPS)-induced DC maturation. By characterizing LPS-induced mature DCs (mDCs) from Tmod1 knockout mice, we found that compared with Tmod1+/+ mDCs, Tmod1-deficient mDCs exhibited lower surface expression of costimulatory molecules and chemokine receptors and reduced secretion of inflammatory cytokines, suggesting that Tmod1 deficiency retarded DC maturation. Tmod1-deficient mDCs also showed impaired random and chemotactic migration, deteriorated T-cell stimulatory ability, and reduced F-actin content and cell stiffness. Furthermore, Tmod1-deficient mDCs secreted high levels of IFN-ß and IL-10 and induced immune tolerance in an experimental autoimmune encephalomyelitis (EAE) mouse model. Mechanistically, Tmod1 deficiency affected TLR4 signaling transduction, resulting in the decreased activity of MyD88-dependent NFκB and MAPK pathways but the increased activity of the TRIF/IRF3 pathway. Rescue with exogenous Tmod1 reversed the effect of Tmod1 deficiency on TLR4 signaling. Therefore, Tmod1 is critical in regulating DC maturation and immune functions by regulating TLR4 signaling and the actin cytoskeleton. Tmod1 may be a potential target for modulating DC functions, a strategy that would be beneficial for immunotherapy for several diseases.

Simultaneous stabilization of Pb and improvement of soil strength using nZVI.

This study demonstrates the feasibility of nanoscale Zero-Valent Iron (nZVI) for simultaneous stabilization of Pb and improvement of soil strength via batch experiments. The soil samples were prepared using slurry and pre-consolidation method at nZVI doses of 0.2%, 1%, 5%, and 10% (by dry weight). The physicochemical and geotechnical properties of Pb-contaminated soil treated by nZVI were analyzed. The results indicate that the contamination of Pb(II) resulted in a notable reduction in the undrained shear strength of soil from 16.85 kPa to 7.25 kPa. As expected, the Pb in exchangeable and carbonate-bound fractions decreased significantly with the increasing doses of nZVI. Meanwhile, the undrained shear strength of Pb-contaminated soil enhanced substantially as the increase of nZVI, from 25.83 kPa (0.2% nZVI treatment) to 69.33 kPa (10% nZVI treatment). An abundance of bubbles, generated from the oxidation of nZVI, was recorded. The mechanisms for simultaneous stabilization of Pb and soil improvement primarily include: 1) the precipitation and transformation of Pb-/Fe-hydrated oxides on the soil particles and their induced bounding effects; 2) the increased drainage capability of soil as the occupation of nZVI aggregates and bubbles in the macropores space and 3) the lower soil density derived from the increase in microbubbles retained in the soil. This study is provided to facilitate the application of nZVI in the redevelopment of contaminated soil.